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Harry S.J. Koleangan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Mathematics Physics

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Journal : Jurnal Ilmiah Sains

 1 
ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT) Grace S. Baud; Meiske S. Sangi; Harry S.J. Koleangan
JURNAL ILMIAH SAINS Volume 14 Nomor 2, Oktober 2014
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.363 KB) | DOI: 10.35799/jis.14.2.2014.6065

Abstract

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.
ANALISIS KUALITAS AIR SUNGAI SARIO KECAMATAN SARIO MANADO SULAWESI UTARA Gorby Corneles Tarima; Jemmy Abidjulu; Harry S.J. Koleangan
JURNAL ILMIAH SAINS Volume 16 Nomor 1, April 2016
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.134 KB) | DOI: 10.35799/jis.16.1.2016.12324

Abstract

ANALISIS KUALITAS AIR SUNGAI SARIO KECAMATAN SARIO MANADO  SULAWESI UTARAABSTRAK Penelitian ini dilakukan untuk menganalisis kualitas air sungai Sario berdasarkan sifat fisika dan kimia sesuai dengan PP No 82 Tahun 2001. Penelitian dilakukan di laboratorium Baristand Industri Manado pada bulan November 2015, dengan 4 sampel air yang diambil pada 4 titik dari hulu sampai hilir sungai Sario Kota Manado menggunakan 7 parameter yaitu kekeruhan, pH, nitat, nitrit, BOD, COD dan DO. Hasil penelitian ini menunjukkan bahwa kualitas air sungai Sario yang sesuai dengan ambang baku mutu air berdasarkan PP No. 82 Tahun 2001 hanyalah pada bagian hulu sampai tengah Sungai sebelum memasuki wilayah Kota Manado, sedangkan pada sekitaran hilir sungai Sario telah tercemar yang berhubungan dengan aktivitas masyarakat dengan diindikasikan berdasarkan konsentrasi BOD, COD dan DO yang buruk dan tidak sesuai dengan ambang baku mutu air berdasarkan PP No. 82/2001. Kata kunci : Kualitas Air, Sungai Sario   ANALYSIS OF RIVER WATER QUALITY SARIO DISTRICT MANADO  NORTH SULAWESI ABSTRACT This study was conducted to analyze the Sario river water quality based on physical and chemical properties in accordance with PP No. 82 of 2001. The study was conducted in laboratory Baristand Industry Manado in November 2015, with four water samples taken at 4 points from upstream to downstream Sario City Manado using 7 parameters: turbidity, pH, nitat, nitrite, BOD, COD and DO.  The results of this study indicate that the water quality in accordance with the Sario river water quality standard threshold under PP No. 82 of 2001 only on the upstream side to the middle of the river before entering the city of Manado, whereas the downstream Area Sario river has been polluted related community activities to be indicated by the concentration of BOD, COD and DO poor and not in accordance with the water quality standard threshold based PP No. 82 of 2001. Keywords: Water Quality, River Sario
Co-Authors Audy Wuntu Gorby Corneles Tarima Grace S. Baud Jemmy Abidjulu Lidya I Momuat Makatipu, Christian D. Meiske S. Sangi Meiske S. Sangi Pakasi, Jeremy Fransisco