Ahmad Fathoni
Program Studi Kimia, Fakultas Sains Dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah Jakarta

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

Asam Protokatekuat dari Ekstrak Etil Asetatbiji Honje (Etlingera elatior) dan Uji Aktivitas Antioksidannya Dede Sukandar; Ibnu Umarudin Umedi; Siti Nurbayti; Tarso Rudiana; Ahmad Fathoni
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 4, No. 1, Mei 2018
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.049 KB) | DOI: 10.15408/jkv.v4i1.7225

Abstract

Telah dilakukan penelitian untuk mengetahui struktur senyawa fenolik yang memiliki aktivitas antioksidan dari ekstrak etil asetat biji honje (E. elatior). Isolasi senyawa fenolik dilakukan dengan metode maserasi, fraksinasi dengan kromatografi kolom gravitasi (KKG), dan kromatografi lapis tipis (KLT). Uji aktivitas antioksidan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl) dan penentuan struktur senyawa menggunakan spektroskopi UV-Vis, FTIR, NMR, dan MS. Isolat yang diperoleh berupa gum kuning sebanyak 18 mg dari 3 kg sampel kering. Hasil uji aktivitas antioksidan menunjukkan isolat dari ekstrak etil asetat memiliki aktivitas yang sangat kuat dengan IC50 1,32 µg/mL. Hasil analisis dengan spektroskopi UV-Vis, FTIR, NMR, dan MS menunjukkan isolat sesuai dengan rumus molekul C7H6O4 yang dikenal dengan asam protokatekuat (asam 3,4-dihidroksi benzoat).DOI:http://dx.doi.org/10.15408/jkv.v4i1.7225
Isolation and Cytotoxic Activity of The β-Carotene Combination of Trigona Honey and Namnam Leaves Extract (Cynometra cauliflora) Ahmad Fathoni; La Ode Sumarlin; Fuady Hanief; Dede Sukandar
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 1 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.8696

Abstract

Abstract: Isolation and cytotoxic activity of the β-carotene combination of Trigona honey and Namnam leaves extract (Cynometra cauliflora) were conducted. The urgency of isolating β-carotene compounds because of their known anticancer activity. Namnam leaves are macerated using methanol, then combined with Trigona honey. Fractionation and isolation of β-carotene in combination samples were conducted out by chromatography method. The β-carotene was analyzed using UV-Vis and FTIR spectrophotometer. The cytotoxic activity test was conducted by using the Brine Shrimp Lethality Test (BSLT) method with mortality analysis using Probit Analysis with SPSS v20 and Lethal Concentration 50 (LC50) as parameters. The result showed that the cytotoxic activity of Trigona honey after combination reached 36.6% with an LC50 value of 168.2 ppm, an increase from the Trigona honey sample (LC50 = 265.2 ppm) and Namnam leaves extract (LC50 = 196.12 ppm). The highest cytotoxic activity belongs to the n-hexane fraction (LC50 = 77.6 ppm). The best eluent for β-carotene isolation is petroleum ether. There was increased cytotoxic activity in isolates (LC50 of 22.85 ppm). Isolates were analyzed by UV-Vis and FTIR and compared with standards compound. FTIR analysis results showed that the isolates had functional groups of -CH3 (v 2850-2960 cm-1), -CH3 aliphatic (v 1350-1470 cm-1), C=C (v 2100-2350 cm-1 and 675-870 cm-1), and C-O (v 1000-1300 cm-1). Thus, the combination of Trigona Honey and Namnam leaf extract can be developed as an alternative chemopreventive agent.Abstrak: Isolasi dan aktivitas sitotoksik β-karoten kombinasi madu trigona dan ekstrak daun namnam (Cynometra cauliflora) telah dilakukan. Urgensi mengisolasi senyawa β-karoten karena telah diketahui adanya aktifitas antikanker. Daun namnam dimaserasi menggunakan metanol, untuk kemudian dikombinasikan dengan madu trigona. Fraksinasi dan isolasi β-karoten pada sampel kombinasi dilakukan dengan metode kromatografi. Kandungan senyawa β-karoten dianalisis menggunakan spektrofotometer UV-Vis dan FTIR. Uji aktifitas sitotoksik dilakukan dengan metode Brine Shrimp Lethality Test (BSLT) dengan analisis mortalitas menggunakan Probit Analysis dengan software SPSS v20 dan Lethal Concentration 50 (LC50) sebagai parameter. Hasil analisis menunjukkan bahwa aktivitas sitotoksik madu trigona setelah dikombinasikan mencapai 36,6 % dengan nilai LC50 sebesar 168,2 ppm, meningkat dari sampel madu trigona dengan (LC50 = 265,2 ppm), dan ekstrak daun namnam dengan (LC50 = 196,12 ppm). Aktifitas sitotoksik tertinggi dimiliki oleh fraksi n-heksana(LC50 = 77,6 ppm). Eluen terbaik untuk isolasi β-karoten adalah petroleum eter. Terjadi peningkatan aktifitas sitotoksik pada isolat hasil pemisahan (LC50 sebesar 22,85 ppm). Isolatdianalisis dengan UV-Vis dan FTIR dan dibandingkan dengan senyawa standar. Hasil analisa FTIR menunjukkan isolat memiliki gugus fungsi -CH3 (v 2850-2960 cm-1), -CH3 alifatik (v 1350-1470 cm-1), C=C (v 2100-2350 cm-1 dan 675-870 cm-1), dan C-O (v 1000-1300 cm-1). Dengan demikian, kombinasi Madu Trigona dan ekstrak daun namnam berpotensi untuk dikembangkan sebagai agen kemoprefentif alternatif.
Characterization of Phytochemicals and Chemical Compounds of Patat Leaves (Phrynium capitatum) as Wrapping Materials for Pesor Doclang Ahmad Fathoni
EduChemia (Jurnal Kimia dan Pendidikan) Vol 6, No 1 (2021)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.848 KB) | DOI: 10.30870/educhemia.v6i1.9189

Abstract

Doclang is a typical food of Bogor. One of the compositions is “pesor” wrapped in patat leaves. Apart from being a food wrapper, patat leaves are used as traditional medicine. Research on the chemical content of patat leaves is still limited and needs to be further developed. In this study, phytochemical testing and chemical content of the extracts of n-hexane, ethyl acetate, and methanol from patat leaves were carried out. Phytochemical tests include alkaloid tests; steroid and terpenoid testing; flavonoid test; saponin test; phenolic hydroquinone; tannin and polyphenol test. Based on phytochemical test results, the extract of patat leaves was known to contain alkaloids, steroids, triterpenoids, flavonoids, saponins, phenolic hydroquinones, and tannins with different distributions at each level of solvent polarity. The instruments used to identify the chemical content of patat leaf extract were GCMS and LCMS QTOF. Through GCMS analysis on n-hexane extract, 114 compounds were identified with a similarity level of ≥ 80% to the library. Among these compounds, confirmed to be terpenoid and steroid compounds. In the LCMS analysis of ethyl acetate extract obtained 25 compounds and 31 compounds for methanol extract. It is suspected that these compounds come from the alkaloid, steroid, flavonoid, saponin, phenolic hydroquinone, or tannin class.
Antioxidant Activity of Mixed Katuk Leaf Extract and Honey Ahmad Fathoni; La Ode Sumarlin; Jane Ratini Putri; Narti Fitriana
EduChemia (Jurnal Kimia dan Pendidikan) Vol 5, No 2 (2020)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.839 KB) | DOI: 10.30870/educhemia.v5i2.7275

Abstract

Antioxidants can be obtained from plants or animals. One of the plants that can be used as a source of antioxidants is katuk leaf (Sauropus androgynus (L.) Merr.) and honey. The content of compounds in both materials that function as antioxidants are phenolic, flavonoids, and vitamin C. This study aims to analyze the levels of total phenolic, flavonoids, vitamin C, antioxidant activity, the effect of different compositions on combination treatments, and analyze the relationship between phenolic levels and vitamin C with antioxidant activity from the test sample. This study used an experimental method with a completely randomized design. Antioxidant activity was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The results showed phenolic levels of all test samples ranged from 0.509-23.205 mg GAE/g samples; flavonoids amounted to 0.027-17.329 mg QE/g samples, vitamin C amounted to 15,917-101,705 mg AA/g samples and IC50 of 114.213-20727.500 mg/l. The combination treatment with more katuk leaf composition can increase the levels of compounds and antioxidant activity. Phenolic levels and vitamin C have a moderate relationship with antioxidant activity with the correlation coefficient values of -0.480 and -0.500.