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All Journal Jurnal Pertanian Agros Agroista: Jurnal Agroteknologi
Elisabeth Nanik Kristalisasi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PENGARUH DOSIS PGPR DAN LCPKS TERHADAP PERTUMBUHAN BIBIT KELAPA SAWIT DI PEMBIBITAN AWAL Elisabeth Nanik Kristalisasi; Umi Kusumastuti Rusmarini; Rifaldi Gravian Perwana
Agros Journal of Agriculture Science Vol 24, No 2 (2022): edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v24i2.1949

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh dosis PGPR dan LCPKS terhadap pertumbuhan bibit kelapa sawit di pembibitan awal. Penelitian dilakukan di PT. Perkebunan Nusantara III yang terletak di desa Sei Meranti, Kecamatan Torgamba, Kabupaten Labuhan Batu Selatan, Sumatera Utara, pada bulan Juni sampai September 2021. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari dua faktor. Faktor pertama adalah dosis PGPR terdiri dari 3 aras (30, 40, dan 50 ml/bibit). Faktor kedua adalah dosis LCPKS terdiri dari 4 aras (0/kontrol, 30, 40, dan 50 ml/bibit).  Hasil penelitian menunjukkan ada interaksi nyata antara aplikasi dosis PGPR dan LCPKS terhadap tinggi bibit, diameter batang, bobot kering akar, bobot segar tajuk, bobot kering tajuk, volume akar, bobot kering tanaman, dan bobot segar tanaman kelapa sawit di pembibitan awal. Kombinasi perlakuan terbaik adalah PGPR 50 ml/bibit dengan LCPKS 50 ml/bibit. Dosis PGPR maupun LCPKS memberikan pengaruh  sama terhadap parameter panjang akar serta bobot segar akar.
INDUKSI KALUS EMBRIOGENIK KELAPA SAWIT PADA MEDIA MS DENGAN PENAMBAHAN 2,4-D DAN AIR KELAPA MUDA Titin Setyorini; Elisabeth Nanik Kristalisasi
AGROISTA : Jurnal Agroteknologi Vol. 3 No. 1 (2019): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.897 KB) | DOI: 10.55180/agi.v3i1.50

Abstract

Embryogenic callus produced from somatic cells has a significant role in clonal propagation and oil palm breeding (assembly of superior varieties) through tissue culture techniques. Induction and development of embryogenic callus were determined by media composition, vitamins, amino acids, and growth regulators (hormones). This study aimed to determine the role of natural and synthetic hormone which supplemented in MS medium to induce embryogenic callus of oil palm. The research was conducted in laboratory of plant tissue culture, Stiper Agriculture Institute, Yogyakarta from February to May 2019. The explants were inoculated in solid MS medium with three treatments, namely MS medium (Murashige and Skoog), MS + synthetic hormone (2,4-Diclorophenoxyacetate), and MS + natural hormone (young coconut water). The results showed synthetic hormone (2,4-Diclorophenoxyacetate) which supplemented in MS medium can induce embryogenic callus of oil palm explants. However, natural hormone (coconut water) has not been able to induce embryogenic callus. Keywords: Callus induction, oil palm, 2,4-D, coconut water
Co-Authors Rifaldi Gravian Perwana Titin Setyorini Umi Kusumastuti Rusmarini