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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JSDH Jurnal Kimia Terapan Indonesia
NI PUTU RATNA AYU KRISHANTI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Penapisan Bakteri Filosfer Penghasil Senyawa Bioaktif Anti Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Padi NURFITRIANI RINA; NI PUTU RATNA AYU KRISHANTI; ALINA AKHDIYA; ARIS TRI WAHYUDI
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.19-24

Abstract

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the important diseases in rice crops in Indonesia. The disease is difficult to be controlled since it attacks the rice plant at different growth stages such as tillering, flowering and ripening. One of the alternatives that could be used to control the disease is by using phyllosphere bacteria as the biocontrol agents. This study aims to isolate, characterize and screen the rice phyllosphere bacteria producing bioactive compounds against Xoo. Phyllosphere bacteria isolated from healthy leaves of rice var. Ciherang by using 4 different media obtained 285 bacterial isolates which were consisted of the 65 isolates of King’s B agar, 86 isolates of Nutrient agar, 81 isolates of Luria-Bertani agar, and 53 isolates of Trypticase Soy agar media. Antagonist test using double layer method showed 58 isolates of phyllosphere bacteria produced bioactive compounds that inhibited the growth of Xoo. Pathogenicity test agaist rice leaf revealed 18 bacterial isolates did not perform their potencies as pathogenic bacteria. Among the 18 non-phytopathogenic bacterial isolates, 14 isolates belong to Gram-positive bacteria and 4 isolates belong to Gram-negative bacteria. Five isolates among Gram positive bacteria were predicted as Bacillus genera. 
Self-Healing Concrete Using Bacteria Calcification from Karst Cave Environment Ananto Nugroho; Agung Sumarno; Luna Nurdianti Ngeljaratan; Deni Zulfiana; Ni Putu Ratna Ayu Krishanti; Triastutil Triastutil; Eko Widodo
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.98 KB) | DOI: 10.14203/jkti.v21i1.411

Abstract

Karst regions in Indonesia have the uniqueness of the landscape and biodiversity. The karst is formed by the dissolution of rocks and the precipitation of mineral. In the cave, there are ornaments of stalactite and stalagmite which are formed by the process of mineral precipitation. We have isolated, screened, and identified the soil bacterium from the cave environment (Lysinibacillus macroides). These bacteria are able to precipitate calcium carbonate and can be developed as a self-healing agent concrete. We investigated the proportions and the properties of mixtures concrete containing lightweight aggregate and volcanic ash impregnated with bacteria. A comparison study was made by concrete cylinders subjected to compressive strength tests with and without the bacteria. It found that the strength of concrete with bacteria decreased by less than 10.56% for 28 days of cured specimens. This study showed that the effects of bacteria on the strength of concrete are not considerable. However, these bacteria are effective to repair in the microcrack less than 0.3 mm
Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest Ni Putu Ratna Ayu Krishanti; Deni Zulfina; Bramantyo Wikantyoso; Arpiwi Zulfitri; Sulaeman Yusuf
Journal of Tropical Life Science Vol. 8 No. 3 (2018)
Publisher : Journal of Tropical Life Science

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Abstract

Actinomycetes are Gram positive bacteria that have been detected in diverse ecological niches. Their member species are known to be a main source of various bioactive compounds. The discovery of Actinomycetes from diverse and unexplored resources has also been linked to increased opportunities to obtain novel bioactive compounds. Insect nest material is being investigated as a new source of novel antimicrobial producing Actinomycetes, which could be harnessed for therapeutic potential. A total of 10 Actinomycetes isolates were collected from the nest of Nasutitermes sp. in Pananjung Pangandaran Nature Reserve. These isolates were evaluated for antimicrobial activity against the challenge bacteria (Eschericia coli, Staphylococcus aureus, Bacillus subtilis, Serratia Marcescens) and fungi (Fomitopsis palustris, Fusarium oxysporum, Trichoderma viridae) by dual culture method. The result revealed that several isolate were active against fungi and bacteria. Isolate Pn-TN2 showed the highest level of antibacterial inhibition and the highest antifungal inhibition with Inhibition Rate value more than 80%. By morphological and 16S rRNA gene sequence analysis strongly suggested that the isolate Pn-TN2 belonged to Streptomyces prasinopilosus. We suggested that termite nest is a potential source of bioactive strains of cultivable Actinomycetes for future biotechnological needs.
Co-Authors Agung Sumarno ALINA AKHDIYA Ananto Nugroho Aris Tri Wahyudi Arpiwi Zulfitri Bramantyo Wikantyoso Deni Zulfiana Deni Zulfina Dewi Liesnoor Setyowati Luna Nurdianti Ngeljaratan NURFITRIANI RINA Sulaeman Yusuf Triastutil Triastutil