Rudi Joelijanto
Department Of Orthodontic, Faculty Of Dentistry, Universitas Jember

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Journal : Health Notions

Antibacterial Activity of Fermented and Non-Fermented Edel Cacao Bean Extract (Theobroma Cacao L.) Against Porphyromonas gingivalis Mulki Nur Maajid; Rina Sutjiati; Rudi Joelijanto
Health Notions Vol 5, No 8 (2021): August
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50805

Abstract

Porphyromonas gingivalis is considered the primary etiologic factor in periodontitis. The administration of antibiotics has so many adverse effects that alternative ingredients are needed with minimal side effects and as an agromedical-based innovation from herbal ingredients abundant in Jember district, namely extracts from cocoa beans. This study aimed to determine the antibacterial activity of fermented and unfermented cocoa bean extract (Theobroma cacao L.) with various concentrations against P. gingivalis. This research was conducted using fermented cocoa bean extract 1%, 2%, 3%, unfermented cocoa bean extract 1%,2%,3%, chlorhexidine as a positive control, aquadest as a negative control. Extracts were made using the Ultrasound-Assisted Extraction method, then tested for antibacterial using the disk diffusion method on Muller Hinton Agar media, four replications, 24 hours incubation, and measuring the inhibition zone with a caliper. The minimum inhibitory concentration of fermented cocoa bean extract was 3%, the size of the inhibition zone was 12.57 mm, the unfermented cocoa bean extract was 2%, the size of the inhibition zone was 11.67 mm. Test the difference using Kruskal walis, significance value 0.001. Antibacterial activity of cocoa bean extract (Theobroma cacao L.) unfermented Edel variety had more potent antibacterial activity against P. gingivalis than fermented one. The results of the Mann-Whitney test between samples showed that there were almost significant differences between the researched samples. Keywords: antibacterial; Porphyromonas gingivalis; cocoa beans
The Effect of Sodium Fluoride Administration on IL-1β and TNF-α Expression on Orthodontic Teeth Movement Rina Sutjiati; Erna Sulistyani; Leliana Sandra Deviade Putri; Rudy Joelijanto; Herniyati Herniyati; Dwi Prijatmoko; Adik Wulandari; Dani Agam Rahmadianto
Health Notions Vol 6, No 3 (2022): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60304

Abstract

Relapse in post orthodontic treatment is common in the community because resorption in the pressure side occurs excessively due to teeth being moved with an orthodontic device. Excessive resorption can be inhibited by using NaF which has been shown to inhibit osteoclast activity, thereby reducing osteoclast numbers. The purpose of this study was to analyze the effect of NaF administration on IL-1β and TNF-α expression of osteoclast in orthodontic teeth movement. The research used experimental-quantitative research type applying Randomized Posttest Only Control Group Design, by selecting the experimental group and the control group randomly. Animal model using 16 male Wistar rats given orthodontic force using NiTi closed coil spring adjusted to give 10 grams/cm2 of mechanical strength, were applied to the palatal in the maxillary incisors of the Wistar rat teeth. Rats were divided into two groups, the control group (C group) and treatment group (T group) given topical NaF application (11.75 ppm). Immunohistochemical examination was performed to calculate IL-1β and TNF-α expression on osteoclasts on the 7th day and 14th day. The administration of NaF reduces IL-1β and TNF-α osteoclast’s expression both on the 7th day (p <0.05) and 14th day (p <0.05). Data were tested by the Kolmogorov-Smirnov test, Levene test and the difference tested Annova followed by LSD (p <0.05). Topical administration of NaF application to the gingival sulcus of male Wistar rats in orthodontic tooth movement can reduce the number of osteoclasts that express IL-1β in the alveolar bone in the pressure area. Topical administration of NaF application to the gingival sulcus of male Wistar rats in orthodontic tooth movement can reduce the number of osteoclasts that express TNF-α in the alveolar bone in the pressure region. Decreased number of osteoclasts expressing IL-1β and TNF-α significantly occurred on the 7th day and 14th day. Keywords: IL-1β; TNF-α; NaF; osteoclast; orthodontic teeth movement
Expression of IL-10 and Caspase-3 in Alveolar Bone after Topical Administration of Sodium Fluoride (Naf) Gel in Orthodontic Tooth Movement Rina Sutjiati; Sulistyani Sulistyani; Erna Sulistyani; Rudy Joelijanto; Annisa Syifa M.; Hasna&#039; Fakhriyah J.
Health Notions Vol 6, No 2 (2022): February
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60206

Abstract

The success of orthodontic treatment can be achieved through tissue, cellular and molecular mechanisms due to mechanical stimuli formed by the pressure side and the tension side. Sodium Fluoride (NaF) is known to increase the bone formation process in the area of attraction in vitro and in vivo. The study aimed to determine sodium fluoride (NaF) against Caspase-3 by osteoblasts and IL-10 by osteoclasts in alveolar bone remodeling on orthodontic tooth movement. The research applied laboratory experiment using 16 male Wistar rats. Rats were divided into two groups; group C: given orthodontic strength without NaF; and group T: given orthodontic strength with NaF. Variable measurements were carried out after the 7th and 14th day. The data was statistically tested using One-Way Anova test analysis (p ≤0.05) to compare the mean number of expressions of both groups C and T. The number of osteoclasts expressing IL-10 was significantly higher in group T when compared to that of group C while the number of osteoblasts expressing Caspase-3 was smaller in group T when compared to that of group C. NaF can increase IL-10 expression in osteoclasts and decrease Caspase-3 expression by osteoblasts on orthodontic tooth movement. Keywords: Caspase-3; IL-10; sodium fluoride (NaF); immunohistichemistry
Sodium Fluoride Administration Enhances Osteocalcin and Osteonectin Expression in Alveolar Bone on the Tension Side on Orthodontic Tooth Movement Rina Sutjiati; Rudy Joelijanto; Devina Yulia; Anjelia Gelli
Health Notions Vol 5, No 6 (2021): June
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn50603

Abstract

Introduction: In the tension side on the orthodontic tooth movement, the bone formation process occurs, played by osteoblasts. Bone formation by osteoblasts in vitro can be increased by administering NaF (sodium fluoride). Speed of bone formation is one of the most important steps in orthodontic tooth movement. Objectives: to analize the effect of NaF administration on the expression of osteocalcin and osteonectin in the strain area in the incisors after orthodontic tooth movement. Methods: The 10 Wistar adult male rats were given 10 grams of orthodontic mechanical strength on the incisors so that the incisors moved distally and were given NaF twice a day, compared to 10 mice in the control group with the same treatment without NaF administration. Results: NaF increase osteocalcin expression by 31.1% on day 7 and 44.8% on day 14, whereas in osteonectin the increase is 48.1% on day 7 and 26.2% on day 14. Conclusions: NaF increases the expression of osteonectin and osteocalcin in mice that were given orthodontic mechanical strength. Keywords: sodium fluoride; immunohistochemistry; osteocalcin; osteonectin
Cocoa Bean Extract Impact on the Number of Osteoblast and Osteoclast in Orthodontic Teeth Movement Rudy Joelijanto; Rina Sutjiati; Sulistiyani Sulistiyani; Sayidah Khadijah; Fitri Arida Sabhatina
Health Notions Vol 6, No 9 (2022): September
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60901

Abstract

In orthodontic therapy, mechanical force produces remodeling. Because the process of bone resorption on tooth movement is larger than that of bone apposition when a normal force is applied, a balance is required by increasing the number of osteoblasts and lowering the number of osteoclasts. The purpose of this study was to look at the ability of cocoa bean extract to assist balance the apposition and resorption of alveolar bone during orthodontic tooth movement. A total of 36 male Wistar rats were separated into three groups of Group K-(untreated rats), K+ (giving an orthodontic mechanical force), and P (giving an orthodontic mechanical force and a dosage of 250 mg/kg BW cocoa bean extract).  Following euthanasia at 7 and 14 days of therapy and osteoblasts and osteoclasts were counted. The results showed that there was an increase in osteoblasts number and a decrease in osteoclasts number in the treatment group, as indicated by the results of calculating the average number of cells in each group, which were supported by statistical tests using SPSS, which revealed significant differences between groups. In orthodontic tooth movement, cocoa bean extract has the ability to enhance the number of osteoblasts while decreasing the number of osteoclasts. Keywords: osteoblasts; osteoclasts; orthodontic tooth movement; cocoa bean extract