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All Journal JBIO: jurnal biosains (the journal of biosciences) JURNAL PengaMAS
Siti Malihatus Sa'adah
UIN Sunan Ampel Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Social Sciences

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DETECTION OF PIG DNA FRAGMENTS IN HALAL UNLABLED LIPSTICK SAMPLES USING CONVENTIONAL PCR Misbakhul Munir; Siti Malihatus Sa'adah; Siti Latifa; Nabila Ayu; Oki Rahmatirta W; Najwa Maulidina P; Ameliora C E; Eko Prasetya; Yuanita Rachmawati
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 1 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i1.23707

Abstract

From a Muslim perspective, it is very important to know the content, raw materials, and processing of the raw materials used in the cosmetic products used. One type of cosmetics that is most often used is lipstick. However, many lipsticks circulating in Indonesia are not equipped with a halal logo. One of the ingredients for lipstick is pork derivatives. These pig derivatives can be detected using PCR. Based on this background, this study aims to test the presence of pig DNA in lipstick samples that have not been certified halal on the market using 4 combinations of pig DNA fragments coding primers by using the conventional PCR method. Five commercial lipstick samples were selected by purposive sampling. DNA isolation was carried out according to the Wizard Promega Universal Kit. The PCR process was carried out with temperature optimization as follows: Predenaturation 98oC: 2 minutes, denaturation of 95oC: 30 seconds, Annealing 61oC: 30 seconds, Extension 72oC: 40 seconds, and Postextension 72oC: 3 minutes. The results showed that of the 5 samples tested by PCR using 5 kinds of primer combination, none of the samples were suspected to contain pork DNA. DNA isolation is the most difficult step in the lipstick sample detection process. Even though the detection result is negative, it is necessary to carry out further tests which become the Gold Standard of DNA-based testing using Real Time PCR.
STRATEGI DAN UPAYA PENGELOLAAN SAMPAH BERBASIS TEKNOLOGI TEPAT GUNA DI DESA TAMANAYU, LUMAJANG Siti Malihatus Sa'adah; Moch. Rega Nur Alamsyah; Moch. Syafrijal Firmansyah; Feby Zulfa Ramadhiana; Fajar Syarif Mubarok; M. Samsul Muarif; Siti Sa'adatul Muniroh; Sitti Muyassaroh; Siti Jauharotun Nisa'; Moch. Bima Akbar; Hastanti Agustin Rahayu
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Publisher : UNIVERSITAS KHAIRUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/pengamas.v6i2.6239

Abstract

Tamanayu is one of the villages that has problems in waste management. The low level of public awareness and the absence of a TPS make people still throw garbage carelessly. Therefore, it is needed a solution that can be used to reduce the number of landfills. The research is conducted to determine alternative solutions that are effective in waste management in Tamanayu village. The research method used is the CBPR (Community Based Participatory Research) method by observing and socializing the Tamanayu village community. Based on the results of observations, conducting socialization, counseling on waste management and providing periodic assistance in collaboration with Environmental Agency (DLH), and presentation about incinerator prototypes environmentally friendly waste (incinerator) are suitable alternative solutions for waste management in Tamanayu village. The community showed enthusiasm and positive response to the results of the socialization and presentation of the prototype of the incinerator, and agreed to achieve the results of the socialization provided. It is hoped that the community will be more active in playing a role in waste management at Tamanayu village in the future.
Co-Authors Ameliora C E Eko Prasetya Fajar Syarif Mubarok Feby Zulfa Ramadhiana Hastanti Agustin Rahayu M. Samsul Muarif Misbakhul Munir Moch. Bima Akbar Moch. Rega Nur Alamsyah Moch. Syafrijal Firmansyah Nabila Ayu Najwa Maulidina P Oki Rahmatirta W Siti Jauharotun Nisa' Siti Latifa Siti Sa'adatul Muniroh Sitti Muyassaroh Yuanita Rachmawati, Yuanita