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STUDI PERAKITAN KELAPA HIBRIDA GSK x DMT BERDASARKAN PENANDA RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Runtunuwu, Semuel D.; Novarianto, Hengky; Tampake, Heldering; Lengkong, Edy F.
EUGENIA Vol 14, No 1 (2008)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.14.1.2008.7431

ABSTRACT Â Runtunuwu, S.D. et al. 2008. Assembling Hybrid Coconut of GSK x DMT Based on RAPD (RANDOMLY AMPLIFIED POLYMORPHIC DNA) Marker. Eugenia 14 (1) : 134-152. Â The aimed of this research was : 1. assembling hybrid coconut GSK x DMT (Genjah Salak x Dalam Mapanget) that seeds growth was relatifly homogeneous based on RAPD (Randomly Amplified Polymorphic DNA) marker and 2. to found the assembling method of hybrid coconut that will produce massive seeds relatifely short time will homogeneous plant. It was 65 individu trees observe for the average of famale flower per bunch. The result was 25 individu of coconut GSK has the average flower production > 40 per bunch was analyze the homogeneous genetic with the RAPD marker. Based on the analyze RAPD that were 25 individu of GSK coconut trees have the same genetic average 88 % and 14 individu among that was 100 % have same genetic. Further more that 14 individu of GSK was crossing with the 3 individu of DMT that have high yield per year its was DMT 1188, 1172 and 781. Based on the evaluation for the color of buds, high of buds, the steam circle, the petiole color and the germination time of hybrid coconut seeds from the crossing of GSK x DMT 1188 produce more thanÂ Â Â 70 % seeds that have same genetic, also for crossing of GSK x DMT 1172 have 9 combination and have more than 70 % that same genetic, 10 combination from crossing GSK x DMT 781 have more than 80 % same seeds growth. Therefore, using the RAPD marker were successfully produced 28 crossing of the hybrid coconut GSK x DMT that have relatifly homogeneous seeds growth. Â Keywords : assembling, hybrid coconut GSK x DMT, RAPD.

IDENTIFIKASI KERAGAMAN GENETIK KENTANG SUPEJOHN TRANSGENIK (Solanum tuberosum L. var supejohn) Lengkong, Christine L.W.; Polii-Mandang, Jeany; Lengkong, Edy F.
EUGENIA Vol 18, No 3 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.3.2012.4095

ABSTRACT Â The aim of this study was to determine the genetic diversity of potato Supejohn transgenic that had been twenty-five times subcultured with explorationÂ method. The genetic variability was calculated using molecular marker Polimorphic Random Amplified DNA (RAPD) analysis of ten samples from four weeks planlets of Supejohn transgenic plants using ten random primers. DNA isolation of 10 samples using CTAB bufferÂ then measurement of the DNA qualification and quantification,Â afterwards amplification of DNA by PCR using 10 random primers followed by electrophoresis on a 1% agarose gel with TAE 1x electrode buffer solution. VisualisationÂ the results used UV Transluminator to see DNA bands and the data ofÂ polymorphic bands had been analized used the NTSYS-pc version of 1.07 program to obtain the similarity coefficientÂ and the dendrogram.The results showed that five random primers produced polymorphic DNA bands with 0.39 - 0.88 similarity coefficientÂ and the average similarity coefficient is 0.63 (63%) or the genetic diversity of the samples as many as 37%. Dendrogram formed eight distinct clusters corresponding similarity coefficient, the formation of clusters means that there is genetic diversity among the DNA samples. Keywords: potato Supejohn transgenic, RAPD, genetic diversity ABSTRAK Penelitian ini bertujuan untukÂ menentukan keragaman genetik kentang Supejohn transgenik yang telah dua puluh lima kali disubkultur dengan menggunakan metode penelitian eksplorasi dengan penanda molekulerÂ Random Amplified Polimorphic DNA (RAPD) terhadap 10 sampel tanaman Supejohn transgenik, berumur empat minggu danÂ menggunakanÂ 10 primer random.Â Isolasi DNA dari 10 sampel menggunakan buffer CTAB sesudah itu dilakukan pengukuran kualitas dan kuantitasÂ DNA, kemudian dilanjutkan dengan amplifikasi DNA secara PCR menggunakan 10 primer random diikuti denganÂ elektroforesis pada gel agarose 1% dengan larutan penyanggah elektroda TAE 1x. Visualisasi hasil elektroforesisÂ menggunakan UV Transluminator untuk melihat pita DNAÂ dan analisisÂ data pita polimorfikÂ menggunakan program NTSYS-pc versi 1.07 sehingga didapatkan koefisien kesamaan dan dendrogram. Hasil penelitian menunjukkan bahwa lima primer random menghasilkan pita DNA polimorfik dengan koefisien kesamaan 0.39 â€“ 0.88 dan rata-rata koefisien kesamaan yaitu 0.63 (63%) atauÂ keragamanÂ genetik sampel sebesar 37%.Â DendrogramÂ membentuk delapan cluster yang berbeda sesuai koefisien kesamaan,terbentuknya clusters mengartikan bahwa ada keragaman genetik antar sampel DNA. Eugenia Volume 18 No. 3Â Desember 2012 Kata kunci : kentang Supejohn transgenik , RAPD, keragaman genetik

PENGGUNAAN PENANDA MOLEKULER RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) UNTUK ANALISIS KERAGAMAN GENETIK KELAPA WEST AFRICAN TALL (WAT) Lengkong, Edy F.; Runtunuwu, S. D.
EUGENIA Vol 11, No 3 (2005)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.11.3.2005.7391

ABSTRACT Lengkong, E.F. and S.D. Runtunuwu. 2005. Use of Molecular Marker Random Amplified Polymorphic DNA (RAPD) to Genetic Diversity Analysis of West African Tall (WAT) Coconut. Eugenia 11 (3):210-217. Â Information on genetic diversity of crop gemplasm has several important implications for plant breeder. Among others is to help the breeders to decide what sources to cross so as to making new genetic combination. The use of molecular marker to genetic diversity analysis on DNA level is usefull, because it provides an opportunity to more precisely measure genetic relationships as well is not affected by the environment. The objective of this research was to analyze genetic diversity of West African Tall (WAT) using molecular marker RAPD. Five arbitrary 10-mer primers were used to amplify total DNA genom, and to generate 44 band DNA with 26 band or 59 % were polymorphic band. It was revealed that genetic diversity within population of WAT coconut was 14 %. Based on cluster analysis, at the genetic similarity 85% or genetic diversity 15 % the population was separated on three clusters. This research concluded that the population of WAT coconut grown from open pollinated seeds has different genotype one each others. Keywords: West African Tall (WAT), Coconut, RAPD

STUDI KERAGAMAN POLA PITA DNA DAN KESERAGAMAN POPULASI KELAPA GENJAH SALAK (GSK) BERDASARKAN PENANDA RAPD Hengky, Novarianto; Runtunuwu, Semuel D.; Lengkong, Edy F.; Singkoh, Merlyn
EUGENIA Vol 13, No 1 (2007)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.13.1.2007.7430

ABSTRACT Novarianto, H., et.al. 2007. The Study of the Variety of DNA Tape Form and the Coconut Genjah Salak Population Variety Based on RAPD Code. Eugenia 13 (1):109-118. Â The goal of this research to study the variety of DNA tape form and the population variety of coconut Genjah Salak (GSK) based on RAPD code (Randomly Amplified Polymorphic DNA). 25 trees individu of coconut GSK was amplification the genomic of DNA using 35 primer Operon Kit A and Kit B. The coconut GSK individu tree that were analyse was selected based on the female flower production, with average above 40 female flower. The electrophoresis of DNA result of amplication PCR get tape form monomorfic and polimorfic between 25 individu coconut GSK trees that was analyzed. OPA 09 primer and OPB 06 get the result of monomorfic tape form, other wise the OPA 13 primer, OPB 05 and OPB 10 get polimorfic tape form. The genetic similarity in coconut Genjah Salak (GSK) population based on RAPD code rate was 88 % or have the variety genetic of 12 %. Therefore based of this similarity the coconut GSK population was clustering in five groups. The goal of this research to study the variety of DNA tape form and the population variety of coconut Genjah Salak (GSK) based on RAPD code (Randomly Amplified Polymorphic DNA). 25 trees individu of coconut GSK was amplification the genomic of DNA using 35 primer Operon Kit A and Kit B. The coconut GSK individu tree that were analyse was selected based on the female flower production, with average above 40 female flower. The electrophoresis of DNA result of amplification PCR get tape form monomorfic and polimorfic between 25 individu coconut GSK trees that was analyzed. OPA 09 primer and OPB 06 get the result of monomorfic tape form, other the OPA 13 primer, OPB 05 and OPB 10 get polimorfic tape form. The genetic similarity in coconut Genjah Salak (GSK) population based on RAPD code rate was 88 % or have the variety genetic of 12%. Therefore based of this similarity the coconut GSK population was clustering in five groups. Keywords: Keragaman genetic; kelapa Genjah Salak (GSK), Randomly Amplified Polymorphic DNA (RAPD)

KAJIAN PENERAPAN PHT DALAM PELESTARIAN LINGKUNGAN PADA PETANI PADI SAWAH DI KABUPATEN BOLAANG MONGONDOW TIMUR Arfa ., Dondo; Max ., Tulung; Edy F. Lengkong
AGRI-SOSIOEKONOMI Vol. 12 No. 3A (2016)
Publisher : Sam Ratulangi University

ABSTRACT This study aims to determine how the behavior of farmers in the implementation of Integrated Pest Management (IPM) in terms of three aspects: the ecological aspects, economic aspects and technological aspects. This research was conducted in two districts in East Mongondow Bolaang. Collecting data in this study conducted at 40 farmers of the District Parent and Modayak Modayak West. All farmers are farmers who have participated in the Field School of Integrated Pest Management. The data collection is done by using a questionnaire. The questionnaire was prepared based on measurement principles Likert Scale. The collected data were analyzed using Multiple Linear Regression Analysis. Based on the research, regression equation: Y = 4.240 + + 0,326X3 0,083X2 0,07X1 + e. Furthermore, based on the results of testing of the F-statistic can be concluded that the ecological aspects, economic aspects and technological aspects together have the same effect on the opinion of the farmer (Y) significantly. This we can see where the F-count is 8,040 and the F-table for 2866, in other words 8,040 F count> F-Table 2866. So the null hypothesis is rejected. It means ecological aspects, economic aspects and technological aspects together have the same effect on the opinion of the farmer (Y). While based on t-test statistics for independent variables namely the ecological aspects, economic aspects and aspects of the technology are as follows: the ecological aspect t calculate equal to 2,422> t table 2028, the economic aspect t calculate equal to 2,083> t table 2,028 and technological aspects t of 2,702> t table 2,028. Thus, ecological aspects, economic aspects and technological aspects of the real effect on the opinion of farmers about IPM.

PREDICTION OF EROSION BY THE METHOD OF USLE (UNIVERSAL SOIL LOSS EQUATION) Martha Fera Garing; Edy F. Lengkong
Jurnal Agroekoteknologi Terapan Vol. 2 No. 1 (2021): EDISI JANUARI-JUNI 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v2i1.34061

Soil as a natural resource has been subjected to various pressure along with an increase in the number of human beings. Such pressure has led to a decrease in the quality of the soil that leads to the reduction of the soil's ability to produce. A decrease in the quality of the soil is caused by the process of leaching of nutrients and the process of soil erosion, especially on land - land that does not have closing vegetation. Erosion is the events of emigration land or portions of land from one place to another by nature. Erosion in the event, the ground or parts of the land eroded and transported, are then deposited in other places.Keywords =Â Â Â erosion; soil.Â AbstrakTanah sebagai sumber daya alam telah mengalami berbagai tekanan seiring dengan peningkatan jumlah manusia. Tekanan tersebut telah menyebabkan penurunan mutu tanah yang berujung pada pengurangan kemampuan tanah untuk berproduksi. Penurunan mutu tanah disebabkan oleh proses pencucian hara dan proses erosi tanah terutama pada lahan- lahan yang tidak memiliki penutupan vegetasi. Erosi adalah peristiwa pindahnya atau terangkutnya tanah atau bagian-bagian tanah dari suatu tempat ke tempat lain oleh media alami. Pada peristiwa erosi, tanah atau bagian- bagian tanah terkikis dan terangkut, kemudian diendapkan di tempat lain.Kata kunci = Â Â pengikisan; tanah.

THE INFLUENCE OF THE TYPE AND CONCENTRATION OF ZPT ON GROWING MEDIA ON THE GROWTH OF PLANTS ORCHID (Dendrobium sp.) Mersi M. Tanawani; Edy F. Lengkong
Jurnal Agroekoteknologi Terapan Vol. 1 No. 2 (2020): EDISI JULI-DESEMBER 2020
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v1i2.34067

Plant propagation can be done by generative and vegetative. Generative plant propagation is usually done through seeds and experience natural pollination with the help of the wind or insects. The advantages of generative plant propagation, the plants have root systems that are strong and sturdy, it is easily propagated, and a fruitful period is long. While the drawbacks are like planting is done at the time of the season, the resulting offspring is likely not the same as its parent, the percentage of germinated low and need a long time to germinate and time to bear fruit for longer.Keywords: propagation; growth regulator; Dendrobium sp.Â AbstrakPerbanyakan tanaman dapat dilakukan dengan cara generatif dan vegetatif. Perbanyakan tanaman secara generatif dilakukan melalui biji dan mengalami penyerbukan alami dengan bantuan angin atau serangga. Keunggulan dari perbanyakan tanaman secara generatif yaitu tanaman memiliki sistem perakaran yang kuat dan kokoh, lebih mudah diperbanyak dan jangka waktu berbuah lebih panjang. Sedangkan kekurangannya yaitu seperti penanaman dilakukan pada saat musimnya, keturunan yang dihasilkan kemungkinan tidak sama dengan induknya, persentase berkecambah yang rendah dan membutuhkan waktu yang agak lama untuk berkecambah dan waktu untuk berbuah lebih lama.Kata kunci: perbanyakan; Â zpt; Â Dendrobium sp.

PENGARUH PENGGUNAAN MULSA PADA PERTUMBUHAN DAN PRODUKSI TANAMAN KENTANG (Solanum tuberosum L.) DI DATARAN MENENGAH Giavirna J. Enoch; Edy F. Lengkong; Jantje Pongoh
COCOS Vol. 9 No. 5 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v1i1.19302

ABSTRACTThe objective of this study was to study the effect of mulch use on the growth and production of potato crops in the middle plains and to determine the type of mulch suitable for potato crops in the middle plains. This research was conducted in Tompaso II Village District Tompaso, Regency of Minahasa, with the height of 765 mdpl. This study was implemented for 4 months, from May to August 2016. This study used a Completely Randomized Design experiment consisting of 3 treatments with different repetitions. The Treatments are Plastic Silver Black Mulch repeated 3 times, Straw Mulch repeated as much as 4 times and Control repeated 6 times. Based on the result of statistical analysis, there is no significant difference between the treatment can be caused by environmental factors and genetic factors seedings are planted. Environmental factors such as temperature, humidity, rainfall, light intensity, soil fertility, and the presence or absence of pests and diseases will determine growth and production in plants.Keywords: the potatos, medium plains, mulch

RESPON PEMBENTUKKAN KALUS EMBRIONIK TANAMAN KRISAN KULO (Chrysanthemum morifolium) TERHADAP PEMBERIAN ZAT PENGATUR TUMBUH SITOKININ Juita S. Sinaulan; Edy F. Lengkong; Stella Tulung
COCOS Vol. 10 No. 3 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v1i1.22203

ABSTRACT Growth regulating substances are organic or inorganic compounds that play an important role in biological processes in plant tissue and one of them is embryonic callus formation and embryogenesis. This study aims to determine the effect of the administration of growth regulators cytokines at certain concentrations on the formation of embryonic callus of chrysanthemum plants. This research was conducted at the Genetics Laboratory of the Faculty of Agriculture, Sam Ratulangi University, Manado. The research was conducted for 4 (four) months, starting June 2018 to October 2018. The research method was carried out using a completely randomized design using the growth regulator from cytokinins, namely zeatin (Z) and TDZ (T) with the concentrations used were 2 ppm, 4ppm and 6ppm, each of which added auxin ZPT 1AA 0.02 Ppm and GA3 O, 2 ppm and repeated 5 times. The variables observed were callus formation time, callus wet weight, and callus color. The results showed that the the callus formation time in chrysanthemum culture was the fastest is 7,8 days after culture, which was treated with zeatin 2 ppm, the largest wet weight culture was 2.111 gr at the treatment of 2 ppm TDZ and the color of the callus formed was green. Keywords : Growth Regulating Substances, Embryonic Callus

MONITORING HAMA KUMBANG BADAK (Oryctes rhinoceros L.) PADA TANAMAN KELAPA (Cocos nucifera L.) MENGGUNAKAN FEROMON DI KECAMATAN MAPANGET KOTA MANADO Tegar P. Prok; Robert W. Tairas; James B. Kaligis; Edy F. Lengkong
COCOS Vol. 11 No. 3 (2019): EDISI JULI-SEPTEMBER 2019
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/cocos.v3i3.28720

ABSTRACT This research aims to determine the population of O. rhinoceros with pheromones and trap devices. This research was carried out in the paniki experimental garden in Mapanget Subdistrict, Manado city, from October to December 2019. This research used a survey method which was carried out directly on coconut plants. Determination of the location of the imago O. rhinoceros sample collection was done by purposive sampling, ie taking the imago O. rhinoceros sample trapped in the pheromone trap. This study uses pheromones (ethyl 4methyloctanoate), for traps made from buckets and installed 4 sides of zinc plates at the top of the bucket, zinc plates made holes to hang pheromones, then at the bottom of the bucket is perforated. This is so that when there is rain, water will not flood the bucket. Traps used consist of traps that are yellow and colorless, traps are then placed on a coconut plantation with a distance between traps 100-150 meters and placed diagonally. Observations are made once a week for 2 months, beetles caught during observation are counted. The results showed that 83 yellow-painted traps could be trapped and unpainted traps could trap 78 individuals. Keywords: O. rhinoceros, pheromones, Coconut

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