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The Intracellular Cryoprotectant Effects in Preserving Goramy Spermatozoa after Two Days Sub-Zero Freezing Abinawanto, Abinawanto; Fitrianingrum, Nisa; Lestari, Retno; Sudaryono, Agung; Rostika, Rita; Fujaya, Yushinta
Aquacultura Indonesiana Vol 16, No 1 (2015): Volume 16 Issue 1 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

The spermatozoa quality of goramy two days after sub-zero freezing was examined. The quality of spermatozoa examined included motility, viability, and abnormality. We aimed to determine the optimum concentration of glycerol protecting spermatozoa during preservation. We used 0%, 1%, 3%, 5%, 7%, and 9% of glycerol, respectively. Sperms were diluted by the combination of glycerol and fish ringer (1 part of sperm + 3 part of solvent). The dilute sperms were then equiliberated at 4°C for 45 min, and were freezed at -34°C for two days. Thawing was then carried out at 30°C for 2 min. Based on Dunnet test, 5% of glycerol was the optimum concentration maintaining spermatozoa motility (75.95±4.76)%.Keywords: glycerol; Osphronemus goramy; spermatozoa motility, viability, and abnormality; sub-zero freezing

Peranan Dimetil Sulfoksida sebagai Krioprotektan dalam Mempertahankan Kualitas Spermatozoa Ikan Gurami, Osphronemus goramy Lacepede, 1801, Dua Puluh Empat Jam Pascakriopreservasi Abinawanto, Abinawanto; Bayu , Mariana D.; Lestari , Retno; Sunarma , Ade
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Penelitian dilakukan dengan tujuan mengetahui pengaruh berbegai konsentrasi DMSO (0%, 5%, 7%, 10%, 13%, 15%, dan 17%) sebagai krioprotektan terhadap kualitas spermatozoa ikan gurami, Osphronemus goramy Lacepede, 1801, dua puluh empat jam pascakriopreservasi. Penelitian dilakukan di Balai Besar Pengembangan Budidaya Air Tawar (BBPBAT), Sukabumi. Semen dikoleksi dengan cara pengurutan (stripping) dan dievaluasi secara makroskopis (warna, pH, dan volume) dan mikroskopis (persentase motilitas, viabilitas, abnormalitas, dan konsentrasi spermatozoa) baik sebelum maupun sesudah kriopreservasi. Semen diencerkan dengan pengencer yang mengandung ekstender 189M dan krioprotektan DMSO (0%, 5%, 7%, 10%, 13%, 15%, dan 17%), disimpan pada cryotube 2 ml dan dikriopreservasi selama 24 jam. Peningkatan konsentrasi DMSO memengaruhi kualitas spermatozoa ikan gurami pascakriopresevasi. Konsentrasi DMSO yang terbaik berdasarkan hasil pengamatan adalah DMSO konsentrasi 13% (P > 0,05) dengan persentase motilitas sebesar 68,58%; abnormalitas sebesar 29%; dan viabilitas sebesar 63,5%.

Lived Experiences of The Recovery Process of People with Schizophrenia During a Pandemic Dharma, I Dewa Gede Candra; Ahsan, Ahsan; Lestari, Retno
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 7, No 1: March, 2022
Publisher : Universitas Aisyah Pringsewu

The existence of social restrictions and activities during the pandemic in the last two years may have hampered the recovery process of people with schizophrenia. The Denpasar City Empowered House Rehabilitation Center is designed as a place for people with schizophrenia in the community to recover, become independent, and be productive. Several studies have attempted to explain how people with schizophrenia recover, but little is known about how this happens during pandemics. Therefore, this study used interpretive phenomenological methods to explore and understand the meaning of life for people with schizophrenia. The participants were 5 people with schizophrenia who were at the Denpasar Empowered House. Furthermore, interviews were conducted using in-depth techniques. The interview data were transcribed verbatim and analyzed using the Creswell approach. The results obtained 8 themes, namely: (1) Feeling cared for by others, (2) Accepted by the community, (3) Having a positive self-identity, (4) Ngayah helping the recovery process, (5) More helpful to explore self-ability, (6) To recover, (7) Enjoy active socializing, and (8) Situations that affect self-ability. It can be concluded that the participants interpret the recovery process as a goal to return to being part of the community during a pandemic.Abstrak: Adanya pembatasan dan aktivitas sosial selama pandemi dalam dua tahun terakhir dapat menghambat proses pemulihan penderita skizofrenia. Pusat Rehabilitasi Rumah Berdaya Kota Denpasar dirancang sebagai wadah bagi penderita skizofrenia di masyarakat untuk pulih, mandiri, dan produktif. Beberapa penelitian telah menjelaskan bagaimana penderita skizofrenia dapat pulih, tetapi sedikit yang diketahui tentang bagaimana proses pemulihan penderita terjadi selama pandemi. Oleh karena itu, penelitian ini menggunakan metode interpretif fenomenologis yang bertujuan untuk menggali dan memahami makna hidup bagi penderita skizofrenia. Partisipan adalah 5 orang penderita skizofrenia yang berada di Rumah Berdaya Denpasar. Selanjutnya, wawancara dilakukan dengan menggunakan teknik wawancara mendalam. Data wawancara ditranskripsikan secara verbatim dan dianalisis menggunakan pendekatan Creswell. Hasil yang diperoleh yaitu sebanyak 8 tema: (1) Merasa diperhatikan oleh orang lain, (2) Diterima oleh masyarakat, (3) Memiliki identitas diri yang positif, (4) Ngayah membantu proses pemulihan, (5) Lebih bermanfaat untuk mengeksplorasi kemampuan diri, (6) Untuk memulihkan diri, (7) Menikmati bersosialisasi secara aktif, dan (8) Situasi yang mempengaruhi kemampuan diri. Kesimpulan penelitian ini adalah penderita skizofrenia dapat memaknai proses pemulihan sebagai tujuan untuk kembali menjadi bagian dari masyarakat selama pandemi.

Cognitive Behaviour Therapy (CBT) Lowers Online Game Addiction Rates in Adolescents Tambunan, Hotma Roiningsih; Kumboyono, Kumboyono; Lestari, Retno
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 8, No S1: Supplement
Publisher : Universitas Aisyah Pringsewu

High curiosity in adolescents is often shown by playing online games excessively. Continuous and excessive use of online games has many negative impacts, including reduced sleep duration, reduced focus on learning, and increased financial costs incurred. Action that can be given to overcome the negative impact of using online games is the provision of psychotherapy. Psychotherapy that can be given and is believed to be able to reduce the level of online game addiction in adolescents is cognitive behavior therapy (CBT). This study aims to identify the benefits of CBT in reducing online game addiction in adolescents. This type of research is quasi-experimental with a nonequivalent control group design. The research subjects were divided into two groups: the intervention group (23 people) and the control group (23 people). The research was conducted at the Malang National Vocational School in September-November 2022. Data were analyzed using the Wilcoxon and Mann-Whitney tests. This study showed that the treatment group's average value of online game addiction statistically decreased with a p-value of 0.000 (p less than 0.05). In contrast, in the control group, there was no difference in the p-value of 0.059. A different test for the two groups yielded a p-value of 0.000. From these results, it can be seen that there is a difference in the level of online game addiction between the treatment and control groups. Giving CBT to adolescents is statistically proven to reduce addiction to online games so that adolescents can improve their quality of life by carrying out positive activities that benefit them. Abstrak: Rasa keingintahuan yang tinggi pada remaja sering ditunjukkan dengan bermain game online secara berlebihan. Penggunaan game online yang dilakukan secara terus menerus dan berlebihan memiliki banyak dampak negatif, antara lain berkurangnya durasi tidur, fokus belajar berkurang, dan meningkatnya biaya finansial yang dikeluarkan. Tindakan yang dapat diberikan dalam mengatasi dampak negatif penggunaan game online yaitu pemberian psikoterapi. Psikoterapi yang dapat diberikan dan dipercaya dapat menurunkan tingkat adiksi game online pada remaja yaitu cognitive behavior therapy (CBT). Studi ini bertujuan mengidentifikasikan manfaat pemberian CBT dalam menurunkan tingkat adiksi game online pada remaja. Jenis penelitian ini adalah quasi-experimental dengan rancangan nonequivalent control group design. Subjek penelitian dibagi menjadi dua kelompok yaitu kelompok intervensi (23 orang) dan kelompok kontrol (23 orang). Penelitian dilakukan di SMK Nasional Malang pada bulan September-November tahun 2022. Data dianalisis menggunakan uji Wilcoxon dan Mann-Whitney. Hasil penelitian ini menunjukan nilai rata-rata tingkat adiksi game online pada kelompok perlakuan secara statistik mengalami penurunan dengan p-value 0,000 (p kurang dari 0,05), sedangkan pada kelompok kontrol tidak terdapat perbedaan p-value 0.059. Uji beda dua kelompok didapatkan hasil p-value 0.000, dari hasil ini terlihat ada perbedaan tingkat adiksi game online antara kelompok perlakuan dan kontrol. Pemberian CBT pada remaja secara statistik terbukti menurunkan tingkat adiksi game online, sehingga remaja dapat meningkatkan kualitas hidup dengan melakukan aktivitas positif yang bermanfaat bagi remaja.

Impact Of Organizational Culture And Job Satisfaction On Nurse Performance In General Hospital : A Path Analysis Firmanda, Giovanni Iga; Ahsan, Ahsan; Lestari, Retno
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 8, No 1: March 2023
Publisher : Universitas Aisyah Pringsewu

The performance of health employees influences hospital quality service standards. Nurses play an essential role in developing and monitoring the quality of healthcare services through organizational culture, job satisfaction, and work motivation. This study aimed to investigate the impact of organizational culture and job satisfaction on nurse performance using work motivation as a moderating variable. This study takes a cross-sectional approach with a quantitative design. The research sample included 131 people who were chosen at random (proportionate stratified random sampling). Questionnaires were used to collect data, and its validity and reliability were tested. Data analysis is done via path analysis. The findings revealed that organizational culture impacted work motivation p=0.000 (p less than 0.05) and job satisfaction. The results showed that there was an influence of organizational culture on work motivation p=0.000 (p less than 0.05), job satisfaction on work motivation p=0.000 (p less than 0.05), organizational culture did not affect performance p=0.629 (p more than 0.05), job satisfaction on performance p= 0.000 (p less than 0.05), work motivation on performance p = 0.000 (p less than 0.05). This demonstrates that work motivation is crucial in boosting nurse performance through organizational culture and job satisfaction. A supportive organizational culture promotes nurses' performance in terms of patient safety and quality. In conclusion, a positive work environment promotes contented nurses, which affects health care quality.

The Effectiveness of Giving Cognitive Behavior Therapy (CBT) and Mindfulness Therapy on Adolescents' Social Anxiety Levels Sukmawati, Yulia; Lestari, Retno; Wardhani, Viera
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 8, No 1: March 2023
Publisher : Universitas Aisyah Pringsewu

Social anxiety is a serious problem occurring in early adolescence characterized by a high level of worry, negative evaluation, and avoidance behavior in various social situations. The existence of fear, worry, and unconsciousness in recognizing negative thoughts that cause avoidance in adolescents with social anxiety problems can be overcome by providing CBT and Mindfulness therapy. This study aims to identify the effectiveness of Cognitive Behavior Therapy (CBT) and mindfulness therapy on adolescent social anxiety problems. this research is a research quasi-experimental pre-test post-test control group design. There were 40 participants in this study, namely 20 students in the control group and 20 students in the treatment group. The results obtained from this study were that there were differences in the effectiveness of giving CBT with a combination of CBT and mindfulness in adolescent social anxiety with a p-value of 0.002 (0.002 less than 0.05) which was marked by a decrease in the average score of social anxiety in the group given the CBT combination intervention and mindfulness were greater compared to adolescents who were only given CBT. So it can be concluded that the effectiveness of giving a combination of CBT and mindfulness is better than giving CBT to adolescents who experience social anxiety problems. Abstrak: Kecemasan sosial merupakan masalah serius terjadi pada usia remaja awal ditandai dengan tingkat kekhawatiran tinggi evaluasi negatif dan perilaku penghindaran dalam berbagai situasi sosial. Adanya ketakutan, kekhawatiran serta ketidak sadaran dalam mengenali pikiran negatif yang menyebabkan penghindaran pada remaja dengan masalah kecemasan sosial dapat diatasi dengan pemberian terapi CBT dan Mindfulness. Penelitian ini bertujuan untuk mengidentifikasi efektifitas pemberian Cognitive Behaviour Therapy (CBT) dan terapi mindfullness pada masalah kecemasan sosial remaja. Penelitian ini adalah penelitian quasi eksperimental pre-test post-test control group design. Terdapat 40 peserta dalam penelitian ini yaitu 20 siswa dalam kelompok kontrol dan 20 siswa dalam kelompok perlakuan. Hasil yang didapatkan dari penelitian ini adalah bahwa ada perbedaan efektifitas pemberian CBT dengan kombinasi CBT dan mindfulness terhadap kecemasan sosial remaja dengan nilai p-value 0.002 (0.002 kurang dari 0.05) yang ditandai dengan penurunan skor rata-rata kecemasan sosial pada kelompok yang diberikan intervensi kombinasi CBT dan mindfulness lebih besar dibandingkan dengan remaja yang hanya diberikan CBT saja. Sehingga dapat disimpulkan bahwa efektifitas pemberian kombinasi CBT dan mindfulness lebih baik dibandingkan dengan pemberian CBT pada remaja yang mengalami masalah kecemasan sosial.

Dealing with Vulnerability During a Pandemic: Pregnant Women's Perspectives Beo, Yosef Andrian; Lestari, Retno; Astari, Asti Melani
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 6, No 4: December 2021
Publisher : Universitas Aisyah Pringsewu

The coronavirus affects the psychological condition of mothers during pregnancy; therefore, they are vulnerable to experience anxiety, stress, and depression during the pandemic. This phenomenological study aims to explore the experiences of pregnant women in dealing with pandemic situations. This qualitative study used an interpretive phenomenological approach and in-depth interviews with 10 pregnant women as participants. The data were transcribed and analyzed using an interpretative phenomenological approach. The results led to 9 main themes, include: (1) considering COVID-19 as scary, (2) anxiety due to increased risk of contracting the virus during pregnancy, (3) increased stress during pregnancy, (4) being constrained due to limited activities, (5) couples becoming the main support, (6) the support of people around creates positive feelings, (7) becomes more disciplined in implementing health protocols, (8) more active in using social media, and (9) familiarize with the changing situation of the pandemic. It can be concluded that pregnant women develop new habits during pandemics, such as seeking support from partners and others, adhering to health protocols, being active on social media, and adapting to the pandemic situation. Virus corona mempengaruhi kondisi psikologis ibu selama kehamilan sehingga mereka sangat rentan mengalami kecemasan, stres, dan depresi selama masa pandemi. Studi fenomenologi ini bertujuan untuk menggali pengalaman ibu hamil dalam menghadapi situasi pandemi. Penelitian kualitatif ini menggunakan pendekatan interpretif fenomenologis dan wawancara mendalam pada 10 ibu hamil sebagai partisipan. Data ditranskripsi dan dianalisis menggunakan pendekatan fenomenologi interpretatif. Hasil penelitian menghasilkan 9 tema utama, antara lain: (1) menganggap COVID-19 sebagai hal yang menakutkan, (2) kecemasan karena meningkatnya risiko tertular virus selama kehamilan, (3) meningkatnya stres selama kehamilan, (4) terkendala karena aktivitas yang terbatas, (5) pasangan. menjadi pendukung utama, (6) dukungan orang sekitar menciptakan perasaan positif, (7) menjadi lebih disiplin dalam menerapkan protokol kesehatan, (8) lebih aktif menggunakan media sosial, dan (9) membiasakan diri dengan perubahan situasi pandemi. Dapat disimpulkan bahwa ibu hamil mengembangkan kebiasaan baru selama pandemi, seperti mencari dukungan dari pasangan dan orang lain, mematuhi protokol kesehatan, aktif di media sosial, dan beradaptasi dengan situasi pandemi.

My Husband is My Strength: A Phenomenological Study of Women After Hysterectomy Sofian, Ridwan; Kumboyono, Kumboyono; Lestari, Retno
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 7, No 3: September 2022
Publisher : Universitas Aisyah Pringsewu

Post-hysterectomy women consider hysterectomy as the loss of femininity which significantly impacts the psychological state of the convalescent. The support and motivation from the family, especially the husband tend to affect the wife's life quality and self-confidence after a hysterectomy. Therefore, this study aims to explore the psychological experiences of post-hysterectomy women in terms of support from their husbands using a qualitative method with a phenomenological approach. The participants were selected through the purposive sampling method with inclusion criteria: (1) Women with the history of hysterectomy, (2) Those at productive age (15-64 years old), and (3) Post-hysterectomy respondents living with their husbands. The data were obtained from in-depth interviews with 6 post-hysterectomy participants, and the analysis was carried out using the Interpretative Phenomenological Analysis Technique. The four themes used were: 1) Fear of being abandoned before the procedure, (2) My husband is my strength, (3) I feel the love of my spouse, (4) Thanks for accompanying me, (5) Accepting what has happened, (6) I wish we always be together, healthy, and happy. The support in the form of providing motivation, encouragement, and affection for post-hysterectomy women strengthens and improve life quality. In conclusion, with partner support, women feel the affection and love, which relatively increase happiness within the family.Abstrak: Wanita paska histerektomi menganggap histerektomi sama dengan kehilangan kewanitaan yang berdampak pada aspek psikologis. Dukungan dari suami akan berpengaruh terhadap peningkatan kualitas hidup wanita paska histerektomi. Dukungan dan motivasi dari keluarga khususnya suami akan mempengaruhi kepercayaan diri wanita. Penelitian ini bertujuan untuk mengeksplorasi pengalaman psikologis yang dialami oleh wanita paska histerektomi dilihat dari aspek dukungan dari suami menggunakan metode kualitatif dengan pendekatan fenomenologi. Partisipan dalam penelitian ini dipilih dengan metode purposive sampling dengan kriteria inklusi: (1) Wanita yang telah menjalani histerektomi, (2) Wanita usia produktif (15-64 tahun), dan (3) Wanita pascahisterektomi yang masih memiliki suami. Data didapat dari proses wawancara mendalam kepada 6 partisipan pascahisterektomi. Selanjutnya analisis data menggunakan teknik Interpretative Phenomenological Analysis Terdapat 4 tema yang ditemukan pada penelitian ini yaitu; 1) Takut ditinggalkan sebelum prosedur, (2) Suami adalah kekuatan saya, (3) Saya merasakan cinta pasangan saya, (4) Terima kasih telah menemani saya, (5) Menerima apa yang telah terjadi, (6) Saya semoga kita selalu bersama, sehat, dan bahagia. Dukungan berupa pemberian motivasi, pemberian semangat, dan juga sebagai penghibur bagi wanita pascahisterektomi akan menjadikan kekuatan tersendiri bagi wanita pascahisterektomi. Dapat disimpulkan bahwa dengan dukungan dari pasangan, wanita akan merasakan kasih sayang dan merasa dicintai oleh pasangan. Perasaan dicintai akan meningkatkan kebahagiaan bersama keluarga.

EFFECT OF SOCIO-ART WORKING GROUP THERAPY IN REDUCING THE SYMPTOMS OF HALLUCINATIONS IN COMMUNITY HEALTH CENTER Lestari, Retno; Ahsan, Ahsan
Public Health of Indonesia Vol. 3 No. 3 (2017): July - September
Publisher : YCAB Publisher & IAKMI SULTRA

Background: Schizophrenia is a chronic illness with considerable impact on individuals and their families. One of the symptoms of schizophrenia is hallucination. The treatment goals are reducing the symptoms and maintaining the recovery from the effects of illness.Objective: This study aimed to analyze the effect of socialization and art-working group therapy in reducing the symptoms of hallucinations.Methods: This study used a pre-experimental design with one-group pretest-posttest involved 60 patients aged between 12 and 55 years diagnosed with schizophrenia in the Community-Integrated Health Care in East Java. The Symptoms of Hallucinations Checklist was used to assess the hallucination symptoms. Data were analyzed using Wilcoxon signed rank test.Results: Findings showed that there was a statistically significance difference between pretest and posttest score after the socialization and art-working group therapy with p-value 0.000 (p<0.05).Conclusion: Socialization and art-working group therapy had a significant effect in reducing the symptoms of hallucinations in patients with schizophrenia.

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