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All Journal Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Siti Muslimah Widyastuti

Fakultas Kehutanan Universitas Gadjah Mada

Author-ID : 3094311

Agriculture, Biological Sciences & Forestry

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Penghambatan Penyakit Damping Off (Rebah Semai) pada Semai Pinus dengan Ekstrak Biji Nyiri (Xylocarpus granatum) Siti Muslimah Widyastuti
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

Forest Development inIndonesiais based on sustainable concept. To support this policy, forest protection must be done based on environmental approach. In this approach the use of synthetic pesticide must be minimized. Consequently it is necessary to find out alternative method to substitute the use of synthetic pesticide. Phytofungicide is one of the alternative for the synthetic pesticide. The aim of the research was to evaluate the potential of nyiri (Xylocarpus granatum Koen) seed as phytofungicide for controlling Fusarium sp., a pathogen of pine damping off disease. Evaluation was done based on the activity of the extracted nyiri seeds collected from Cilacap mangrove in inhibiting the spore germination and germ tube length in the laboratory, and suppressing the disease intensity in green house. The result showed that the extract of nyiri had antifungal activity against the pathogen tested. This is the first report on the activity of nyiri as phytofungicide for plant pathogens.

Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium oxysporum f.sp. cubense) dengan Trichoderma sp. Albertus Sudirman; Christanti Sumardiyono; Siti Muslimah Widyastuti
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 1 (2011)
Publisher : Universitas Gadjah Mada

The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc) in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag), second factor was time of Trichoderma culture application (2 weeks before Foc inoculation, at same time with Foc inoculation and 2 weeks after Foc inoculation). Trichoderma sp. was cultured in mixed rice brand and chaff medium. The disease intensity was observed with scoring system of wilting leaves (0–4). The results showed that Trichoderma sp. was antagonistic against Foc in vitro and inhibited 86% of Foc colony development. Mechanism of antagonism between Trichoderma sp. and Foc was hyperparasitism. Trichoderma hyphae coiled around Foc hyphae. Lysis of Foc hyphae was occurred at the attached site of Trichoderma hyphae on Foc hyphae. Added banana seedling with Trichoderma sp. Culture reduced disease intensity of Fusarium wilt. Suggested dose of Trichoderma culture application in glass house was 25 g/polybag, given at the same time with Foc inoculation.

Optimasi Produksi Endokitinase dari Jamur Mikoparasit Trichoderma reesei Harjono Harjono; Siti Muslimah Widyastuti
Jurnal Perlindungan Tanaman Indonesia Vol 7, No 1 (2001)
Publisher : Universitas Gadjah Mada

The objective of the experiment was to select optimal medium composition to induce endochitinase production of Trichoderma reesei. Growing media were prepared by substitution of 0.5% colloidal chitin, 0.5% colloidal chitin and 1% Polyvinyl Pyrrolidon (PVP), 1% crab shell chitin (Sigma), 1% crab shell chitin and 1% PVP into Richard’sMedium (RM). Selected bioagent T. reesei isolate 13 was grown onto the media and the specific activity of endochitinase was measured. The results showed that T. reesei grown in all media performed enzyme expression. The highest specific activity of endochitinase was detected on T. reesei grown in RM medium substituted with colloidal chitin and PVP, followed by the same fungi in RM medium substituted with crab shell chitin plus PVP, colloidal chitin and crab shell chitin alone.

Toleransi Tanaman Peneduh Polyalthia longifolia dan Pterocarpus indicus terhadap Ganoderma sp. Siti Muslimah Widyastuti; Harjono .; I Riastiwi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Susceptibility of Urban Trees Polyalthia longifolia and Pterocarpus indicus to Infection of the red root rot fungus Ganoderma sp. Urban trees on the Gadjah Mada University (UGM) area play an important role in increasing environmental qualities as well as in supporting the teaching and learning processes. However, red root rot disease caused by Basidiomycete Ganoderma sp. has severely infected some existing urban trees. This experiment was aimed to determine the susceptibility of Polyalthia longifolia (glodokan) and Pterocarpus indicus (angsana) to the infection of Ganoderma sp. Identification of infected trees was performed in UGM area. Further steps were carried out to achieve those objectives : (1) isolation of Ganoderma spp. and testing of Koch’s postulate and (2) examination of the susceptibility of P. longifolia and P. indicus to infection of Ganoderma sp. The susceptibility test of P. longifolia and P. indicus to Ganoderma sp. indicated that P. longifolia was more resistant to fungal pathogen infection than that of P. indicus. Based on this experiment, it can be concluded that P. longifolia is a species that is more suitable than P. indicus. P. longifolia should be planted on the areas that have been infested with inocula of Ganoderma sp..

Pengaruh Musim terhadap Perkembangan Atelocauda digitata, Penyebab Penyakit Karat pada Acacia auricaliformis di Yogyakarta Siti Muslimah Widyastuti; . Harjono; Zjakiyah Ari Susanti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

ABSTRACTSeasonal effect on the growth of Atelocauda digitata, the cause of rust disease on Acacia auriculiformis in Yogyakarta. The research was aimed to determine the growth of Atelocauda digitata which attacks Acacia auriculiformis in Gunung Kidul, Yogyakarta and determine the process of A. digitata infection on A. auriculiformis. Quantification of the presence of pathogen and the damage as part of tree health monitoring was represented by the percentage of disease incidence and severity, that was used as a benchmark of A. digitata dynamics. Pathogenic organisms were confirmed by pathogenicity test. The process of infection was studied microscopically and macroscopically. Percentage of disease incidence and severity of all three observation plots during the rainy and the dry season were dynamic, with the highest damage discovered in Plot 5. A. digitata infecting A. auriculiformis produces three types of spores, namely teliospores, uredospores and aeciospores. The hyphae infected the leaf tissue two days after inoculation through stomata. Intracellular hyphae were found five days after inoculation.

Co-Authors . Harjono Albertus Sudirman Christanti Sumardiyono Harjono . Harjono Harjono I Riastiwi Zjakiyah Ari Susanti

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