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Sismindari Sismindari
Fakultas Farmasi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Kloning Gen cry dari Bacillus turingiensis var. kurstaki Nur Rahmi Ardiani; Sismindari Sismindari; Sudjadi Sudjadi
Jurnal Perlindungan Tanaman Indonesia Vol 4, No 2 (1998)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9057.993 KB) | DOI: 10.22146/jpti.9918

Abstract

Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene.
Eksplorasi, Pengujian, dan Identifikasi Khamir Antagonis terhadap Patogen Antraknos (Colletotrichum lagenarium) pada Semangka Kardi Raharjo; Christanti Sumardiyono; Nursamsi Pusposendjojo; Sismindari Sismindari
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11964

Abstract

Phyllosphere is one of habitat of yeast and the other microorganism included an antagonistic microorganism. The antagonistic yeasts have been isolated from watermelon phyllosphere on YM Agar medium. There are 30 isolates of yeast have been found. The pure culture of virulent isolate 115 of C. lagenarium used for testing the antagonistic ability of yeast isolates. Two yeast isolates namely K10 and K15 have highest ability to inhibit growth and development of C. lagenarium colony. From microscopic observation, the isolates K10 and K15 caused deflated on C. lagenarium hyphe. The result of identification of K10 is Candida sp. and K15 is Sirobasidium sp.
Co-Authors Christanti Sumardiyono Kardi Raharjo Nur Rahmi Ardiani Nursamsi Pusposendjojo Sudjadi Sudjadi