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All Journal Journal of Tropical Biodiversity and Biotechnology Journal Pharmasci (Journal of Pharmacy and Science)
Nabilah Istighfari Zuraidassanaaz
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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Callus induction and secondary metabolite profile from Elephantopus scaber L. Junairiah Junairiah; Diah Ayu Wulandari; Edy Setiti Wida Utami; Nabilah Istighfari Zuraidassanaaz
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59234

Abstract

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol.  Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.
Effect of Plant Growth Regulators on Callus Induction in Piper betle L. var. Nigra: Pengaruh Berbagai Jenis ZPT terhadap Induksi Kalus Piper betle L. var. Nigra Junairiah Junairiah; Ni'matuzahroh Ni'matuzahroh; Nabilah Istighfari Zuraidassanaaz; Lilis Sulistyorini
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 8 No. 1 (2023): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v8i1.302

Abstract

Callus culture is one of the plant tissue culture techniques that used to study aspects of plant nutrition, somatic embryogenesis, cell suspension culture, secondary metabolite production, and genetic transformation. Callus induction was carried by adding growth regulators. Black betel (Piper betle L. var. nigra) is a medicinal plant that has the potential to produce secondary metabolites. The aim of this study was to obtain the best formula for callus induction of P. betle L. var. nigra. The single growth regulator used is 2,4-Dichlorophenoxyacetic acid (2,4-D), Benzyl Amino Purine (BAP), Indole Butyric Acid (IBA), Indole Acetic Acid (IAA), and Naphthalene Acetic Acid (NAA) with variations the concentration used is 0; 0.5; 1; 1.5; 2; 2.5 mg/L. Each treatment consisted of 6 replicates grown using Murashige and Skoog (MS) medium in vitro for eight weeks. The results showed that the 1.5 mg/L 2,4-D treatment produced the highest fresh weight (1389.5 mg) and dry weight (55.7 mg). Callus P. betle L. var. Nigra in various treatments showed various textures such as compact and friable with callus colors such as white, greenish-white, brownish-white, yellowish-green, greenish-yellow, brownish-yellow, greenish-brown, brown, and gray.
Co-Authors Diah Ayu Wulandari Edy Setiti Wida Utami Junairiah Junairiah Lilis Sulistyorini Ni'matuzahroh Ni'matuzahroh