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All Journal Journal of Tropical Biodiversity and Biotechnology Journal Pharmasci (Journal of Pharmacy and Science)
Nabilah Istighfari Zuraidassanaaz
Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology

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Journal : Journal Pharmasci (Journal of Pharmacy and Science)

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Effect of Plant Growth Regulators on Callus Induction in Piper betle L. var. Nigra: Pengaruh Berbagai Jenis ZPT terhadap Induksi Kalus Piper betle L. var. Nigra Junairiah Junairiah; Ni'matuzahroh Ni'matuzahroh; Nabilah Istighfari Zuraidassanaaz; Lilis Sulistyorini
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 8 No. 1 (2023): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v8i1.302

Abstract

Callus culture is one of the plant tissue culture techniques that used to study aspects of plant nutrition, somatic embryogenesis, cell suspension culture, secondary metabolite production, and genetic transformation. Callus induction was carried by adding growth regulators. Black betel (Piper betle L. var. nigra) is a medicinal plant that has the potential to produce secondary metabolites. The aim of this study was to obtain the best formula for callus induction of P. betle L. var. nigra. The single growth regulator used is 2,4-Dichlorophenoxyacetic acid (2,4-D), Benzyl Amino Purine (BAP), Indole Butyric Acid (IBA), Indole Acetic Acid (IAA), and Naphthalene Acetic Acid (NAA) with variations the concentration used is 0; 0.5; 1; 1.5; 2; 2.5 mg/L. Each treatment consisted of 6 replicates grown using Murashige and Skoog (MS) medium in vitro for eight weeks. The results showed that the 1.5 mg/L 2,4-D treatment produced the highest fresh weight (1389.5 mg) and dry weight (55.7 mg). Callus P. betle L. var. Nigra in various treatments showed various textures such as compact and friable with callus colors such as white, greenish-white, brownish-white, yellowish-green, greenish-yellow, brownish-yellow, greenish-brown, brown, and gray.
Co-Authors Diah Ayu Wulandari Edy Setiti Wida Utami Junairiah Junairiah Lilis Sulistyorini Ni'matuzahroh Ni'matuzahroh