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All Journal Journal of Tropical Biodiversity and Biotechnology
Indah Juwita Sari
Department of Biology Education, Faculty of Teacher Training and Education, Universitas Sultan Ageng Tirtayasa, Serang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Screening and Identification of Mushrooms Growth Promoting Bacteria on Straw Mushrooms (Volvariella volvacea) Indah Juwita Sari; I Nyoman Pugeg Aryantha
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.60618

Abstract

This research aimed to identify the indigenous Mushroom Growth Promoting Bacteria (MGPB) bacteria that can increase the growth of Volvariella volvacea. The research began by isolating indigenous MGPB from planting media of straw mushrooms in Karawang, Indonesia. The screening was performed to select bacterial isolates that can promote the highest growth of mushrooms by dual culture method on PDA media. There were 10 of the 58 highest bacterial isolates that have a positive effect on the vegetative growth of mushrooms. The 23K bacterial isolate was the most significant increase in mycelium growth compared to other isolates and bacteria-induced controls. A bacterial isolate 25K by gene analysis was identified by 16S rRNA (518F primer (5’- CCA-GCA-GCC-GCG-GTA-ATA-CG -3’) and 800R primer (5’- TAC-CAG-GGT-ATC-TAA-TCC -3’). The result from gene analysis shows that there are ~1550 base pairs products. BLAST analysis and phylogenetic tree adjustment results show that the closest diversity of this bacterial isolate 25K is Bacillus thuringiensis serovar konkukian str. 92-27 (equality value = 99%).
Co-Authors I Nyoman Pugeg Aryantha