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Agung Endro Nugroho
Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta
Medicine & Pharmacology

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The effects of PGV-1 and PGV-2 on the b-hexosaminidase release from intraceluller calcium ion-induced mast cells Agung Endro Nugroho; Sardjiman .; Kazutaka Maeyama
Indonesian Journal of Pharmacy Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.931 KB) | DOI: 10.14499/indonesianjpharm0iss0pp207-216

Abstract

PGV-1 or 2,5-bis(4'-hydroxy-3',5'-dimethylbenzylidene)cyclopentanone and PGV-2 or 2,5-bis(4'-hydroxy-3',5' diethylbenzylidene)cyclopentanone are two benzylidene cyclopentanone analogues of curcumin. In our study, we investigated the effects of these compounds on the b-hexoaminidase enzyme release from mast cell culture (RBL-2H3 cell line). Thapsigargin and ionomycin were used as intracellular calcium ion stimulants for inducing b-hexoaminidase enzyme release from mast cells. The release of b-hexoaminidase enzyme was determined by colorimetric methods with substrate, p-nitrophenyl-2-acetamido-2-deoxy-b-D-glucopyranocide, and a microplate reader at 405 nm. In present study, treatment of 0.5 mM thapsigargin or 1 mM ionomycin could stimulate the release of b-hexoaminidase enzyme from RBL-2H3 cells by 43.91 ± 1.30 % and 52.93 ± 2.07 %, respectively. PGV-1 and PGV-2 showed inhibitory effects on the b-hexoaminidase enzyme release from RBL-2H3 cells induced by the increase of intraceluller calcium ion in dose dependent manner. At the dose of 100 mM, PGV-1 and PGV-2, respectively, inhibited the b-hexoaminidase enzyme release by 73.51 ± 8.69 % and 66.42 ± 8.63 % on thapsigargin experiments; and by 89.73 ± 3.23 % and 38.57 ± 5.32 % on ionomycin experiments. The IC50 values of their effects on the b-hexoaminidase enzyme release from RBL-2H3 cells, respectively, were 22.20 mM and 22.27 mM on thapsigargin experiment; and 22.77 mM and >100 mM on ionomycin experiment. Based on the results, the inhibitory effect of PGV-1 and PGV-2 on the b-hexoaminidase enzyme release from RBL-2H3 cells involving mechanisms related to the alteration on activation processes of intracellular calcium ion on mast cells.Key words : Curcumin, PGV-1, PGV-2, mast cells, b-hexoaminidase enzyme
The anticarcinogenic activity of plants compounds Sugiyanto .; B. Sudarto; Edy Meiyanto; Agung Endro Nugroho; Umar A. Jenie
Indonesian Journal of Pharmacy Vol 14 No 4, 2003
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.227 KB) | DOI: 10.14499/indonesianjpharm0iss0pp216-225

Abstract

The study was conducted to observe the effect of extracts of ngokilo (Gynura procumbens), beluntas (Pluchea indica), murbei (Morus alba) dan tapak doro (Vinca alba) leaves. Showed anticarcinogenic activity on lung tumor growth of mice. In the nex step, compounds having anticarcinogenic effect was isolated and identified, and evaluated on the cultures of meilomaand Vero cells. The results showed that non-polar fraction of ethanol extract of ngokilo leaves did not have anticarcinogenic activity, whereas the polar fraction show anticarcinogenic activity. At least there were three flavonoids (flavon or flavonol groups) in this polar fraction. It was only two of these flavonoids inhibit the growth of myeloma and Vero cells.Key words : ngokilo, Gynura procumbens, anticarcinogenic, flavonoid.
Effect of ethanol extract of Erythrina fusca Lour (cangkring) leaves on suppression of cyclooxygenase-2 expression in raji cell line Zullies Ikawati; Agung Endro Nugroho; Winda Werdhinindah
Indonesian Journal of Pharmacy Vol 17 No 2, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.798 KB) | DOI: 10.14499/indonesianjpharm0iss0pp85-90

Abstract

COX-2. COX-2 is expressed highly in inflammation and cancer cells. The effect of ethanol extract of Erythrina fusca Lour (cangkring) toward suppression of COX-2 expression in Raji cells was studied using immunocytochemistry method. This research showed that ethanol extract of E. fusca Lour leaves on concentration of 250,0; 125,0; dan 62,50 μg/mL was able to suppress the COX-2 expression significantly in comparison to control, with the inhibition of 70,19 ± 2,14 %; 44,69 ± 1,62 %; and 23,25 ± 2,21 %.Key words: COX-2 expression, anti-inflammation, anti cancer, Raji cells, ethanol extract of E. fusca leaves, immunocytochemistry
Co-Authors B. Sudarto Edy Meiyanto Kazutaka Maeyama Sardjiman . Sugiyanto . Umar A. Jenie Winda Werdhinindah Zullies Ikawati