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Rumiyati .

Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta

Author-ID : 3097115

Medicine & Pharmacology

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TOXICITY OF THE AQUEOUS EXTRACTS FROM THE FRUITING BODY OF GANODERMA SP. BY BRINE LETHALITY TEST OF Artemia salina Leach. Rumiyati .; Sismindari .; S.M. Widyastuti
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The level of toxicity of aqueous extract from Ganoderma sp. fruiting body isolated from different plant media was determined in this experiment using Brine shrimp lethality test (BST) on Artemia salina Leach. The influence of media on the toxicity level of aqueous extract from Ganoderma sp. fruiting body was determined in this experiment. Ganoderma sp. and G. Lucidum were grown on three flamboyant, (Delonix regia Bojer ex Hook Rafin), sengon (Paraserianthes falcataria (L) Nielsen) and coconut(Cocos nucifera Linn.) plant media. The aqueous extracts were prepared from fruiting body using 0.02 M phosphate buffer pH 7.2. The toxicity level of these aqueous extract were then screened using the BST method. The level of the toxicity was determined by LC50. The results indicated that the aqueous extracts of Ganoderma sp. and G. lucidum isolated from flamboyant, sengon and coconut plant media were influenced by plant media. Ganoderma sp. was isolated from flamboyant plant media possesed the highest toxicity (LC50 480 g aqueous extract/ml), then followed by Ganoderma sp. isolated from sengon plant media (LC50 770 g aqueous extract /ml) and coconut (LC50 1040 g aqueous extract /ml). G. lucidum from coconut plant media possesed the highest toxicity (LC50 660 g aqueous extract /ml), followed by G. lucidum were isolated from sengon plant media (LC50 1100 g aqueous extract /ml) and flamboyant (LC50 1970 g aqueous extract /ml). It was suggested that G.lucidum having highest toxicity might produce compounds having antitumor activity.Key words: Ganoderma sp., aqueous extracts, toxicity .

Effect of protein fraction of Carica papaya L. leaves on the expressions of p53 and Bcl-2 in breast cancer cells line Rumiyati .; Sismindari .; Ariyani .
Indonesian Journal of Pharmacy Vol 17 No 4, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Carica papaya L. leave was known containing Ribosome-inactivating proteins (RIPs), which demonstrated to have an in vitro cytotoxic effect on cancer cell lines. This researh examined the effect of protein fraction containing RIPs isolated from Carica papaya L. on the expressions of p53 and Bcl-2, regulator proteins on apoptosis process, in breast cancer cell lines (T47D).RIPs from Carica papaya L. leaves were isolated by amonium sulfat precipitation. This fraction was analyzed by the activity of cleaving supercoiled double stranded DNA, in order to identify the presence of RIP. The active fraction was then tested of the citotoxic activity on breast cancer cell lines followed by analysing the expressions of p53 and bcl2 using immunohistochemistry technique.The results indicated that the protein fraction possessed cytotoxic activity in breast cancer cell line with the IC50 of 2.8 mg/mL. The expression level of p53 was increased by 59.4%, while Bcl-2 protein was \ decreased by 63%. These results suggested that this protein could induce apoptotic process.Key words : protein fraction, Carica papaya L. , expression of p53 and Bcl-2

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