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All Journal INDONESIAN JOURNAL OF PHARMACY
Dwisesaria Irma Lena
Faculty of Pharmacy, Andalas University, Kampus Limau Manis, Padang -25163 PO. Box 143, Indonesia
Medicine & Pharmacology

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DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR DETERMINATION AND QUANTIFICATION OF RUBRAXANTHONE IN STEM BARK EXTRACT OF MANGOSTEEN Meri Susanti; Dwisesaria Irma Lena; Dachriyanus Dachriyanus
Indonesian Journal of Pharmacy Vol 25 No 4, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (940.042 KB) | DOI: 10.14499/indonesianjpharm25iss4pp237

Abstract

Rubraxanthone is one of antibacterial  and antioxidant compound which is isolated from stem bark of mangosteen (Garcinia mangostana Linn.). The precise method for analysis of plant constituents is normally a reverse-phase high performance liquid chromatographic (RP-HPLC). Development and validation of a RP-HPLC method for chromatographic separation was carried out on a reversed-phase column Shimadzu ®  Shimp-pack VP – ODS (4.6x250mm) using an isocratic mobile phase of  0.4% formic acid - acetonitrile at  a flow rate 1mL min-1, and detection was  with a UV detector. The linearity of  the proposed method was found in the range of 2.5-25μg/mL-1 with regression coefficient 0.999. Intraday precision studies showed the relative standard deviation ≤1.58% and  inter-day ≤3.20%. Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was 102.18%. The Limit of Detection Limit of Quantitation (LOQ) and were 0.47 and 1.56μg/mL-1.The contents of rubraxanthon in the crude was 0.23±0.07%w/w.
Co-Authors . Dachriyanus Meri Susanti