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All Journal Microbiology Indonesia BERITA BIOLOGI BioEksakta : Jurnal Ilmiah Biologi Unsoed
ENDANG SRIMURNI KUSMINTARSIH
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Population Density of Wolbachia Bacteria and the Induction of the Popcorn-Effect in Drosophila melanogaster ENDANG SRIMURNI KUSMINTARSIH
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.834 KB) | DOI: 10.5454/mi.2.2.7

Abstract

Drosophila melanogaster is known to harbour Wolbachia bacteria that cause the early death of its adult host. The death occurs early as a result of cell rupture caused by the proliferation of Wolbachia within it. However, the number of Wolbachia causing the early death in D. melanogaster is unknown. In order to determine whether the number of Wolbachia is related to the early death of its host, the quantity of Wolbachia and nuclei were examined using the real-time PCR procedure. The results showed that an egg cell contains at least 2-3 bacterial cells. A 4 day old larva contains at least 45 cells. The number of Wolbachia in adult flies of 1, 5, 8, and 12 days old were 422, 535, 964, and 610 per host cell, respectively.
DETEKSI MOLEKULER VIRUS DENGUE SEROTIPE 3 PADA NYAMUK Aedes aegypti DI WILAYAH PURWOKERTO TIMUR Priskila Agnesia Prayitno; Endang Srimurni Kusmintarsih; Daniel Joko Wahyono
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.398 KB) | DOI: 10.20884/1.bioe.2020.2.2.1826

Abstract

Penyakit Demam Berdarah Dengue (DBD) adalah penyakit menular yang disebabkan oleh virus Dengue dan ditularkan melalui gigitan nyamuk Aedes aegypti. Nyamuk tersebut merupakan vektor utama dalam penularan virus Dengue (DENV) dengan ciri khas tubuh dan tungkainya ditutupi sisik dengan garis-garis putih keperakan. Persebarannya luas di daerah tropis dan subtropis, Purwokerto termasuk daerah endemis DBD dan sekaligus ditemukan nyamuk sebagai vektornya. Kecamatan Purwokerto Timur menempati urutan tertinggi dari banyaknya kejadian DBD di wilayah Banyumas, bahkan kejadian luar biasa (KLB) yang terjadi di Kelurahan Sokanegara pada tahun 2016 hingga menyebabkan kematian. Penelitian sebelumnya melaporkan bahwa virus Dengue yang paling banyak ditemukan di Purwokerto adalah serotipe 3. Oleh karena itu, deteksi molekuker nyamuk Ae. aegypti perlu dilakukan berkaitan dengan prediksi penularan Dengue untuk memperoleh informasi awal dalam pencegahan dan pengendalian DENV.Tujuan penelitian ini adalah untuk mendeteksi virus Dengue serotipe 3 pada nyamuk dewasa Ae. aegypti sebagai vektornya. Metode penelitian yang digunakan yaitu metode survei dengan pendekatan secara cross sectional dan teknik pengambilan sampel purposiveAnalisis data survei dilakukan dengan melihat positivitas DENV serotipe 3 pada nyamuk. Hasil penelitian menunjukkan DENVserotipe 3 tidak terdeteksi pada nyamuk yang di sampling di Purwokerto Timur. Kata kunci : Aedes aegypti, Demam Berdarah Dengue, DENV
Deteksi Molekuler Virus Chikungunya pada Nyamuk Aedes aegypti Menggunakan Metode Two Step RT PCR Alvira Rifdah Sativa; Endang Srimurni Kusmintarsih; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.752 KB) | DOI: 10.20884/1.bioe.2020.2.2.1834

Abstract

The Chikungunya fever is a disease caused by an Alphavirus from the family Togaviridae with the symptoms of a patient's posture that is bent over by severe joint pain (arthalgia). Chikungunya disease can be transmitted to humans through an Aedes aegypti as a vector. The outbreak of Chikungunya in Indonesia was first reported in 1973 in Samarinda and then spread to various other regions. Surveillance data show almost every year of the outbreak occurred in various regions in Indonesia. In 2013 there was the outbreak of Chikungunya in North Purwokerto, especially in Bancarkembar and Grendeng.Until now, the medicine or vaccine has not been found to prevent Chikungunya disease. The purpose of this research is to know the infection of virus Chikungunya on Ae. aegypti. This research was conducted by surveying methods with sampling techniques using purposive sampling The observed parameter is th positivity of virus Chikungunya. Analysis data by observing the appearance of DNA band on UV Transilluminator. The results that the amplikon cDNA CHIKV is not detected by the two step RT-PCR method. Key Words : Aedes aegypti, Chikungunya, DNA, Vector
Deteksi Molekuler Virus Dengue dan Chikungunya pada Nyamuk Aedes spp. di Kecamatan Cilongok Dwi Iva Fitriana; Endang Srimurni Kusmintarsih; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.673 KB) | DOI: 10.20884/1.bioe.2020.2.2.1815

Abstract

Kecamatan Cilongok merupakan salah kecamatan endemis DBD dan pernah mengalami KLB chikungunya. Deteksi virus pada nyamuk sebelum menginfeksi manusia penting sebagai peringatan dini dalam upaya pencegahan wabah di daerah endemis. Tujuan penelitian ini adalah mengetahui infeksi virus Dengue dan Chikungunya pada nyamuk Aedes spp. asal kecamatan Cilongok. Penelitian ini dilakukan di empat desa di Kecamatan Cilongok yang meliputi Desa Cilongok, Pernasidi, Kalisari, dan Jatisaba. Desain penelitian ini adalah studi purposive random sampling . Deteksi virus Dengue dan Chikungunya pada nyamuk dilakukan menggunakan teknik Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR). Hasil positif virus dianalisis secara deskriptif untuk menggambarkan potensi transmisi virus. Hasil penelitian menunjukkan bahwa nyamuk Aedes spp. yang tertangkap tidak mengandung virus Dengue dan Chikungunya. Kata kunci: Aedes spp., chikungunya virus, dengue virus, teknik RT-PCR
Shoot Tip Culture: A Potential In Vitro Culture Multiplication of Screw Pines (Pandanus tectorius Park.) Ikhsan - Matondang; Sisunandar .; Alice Yuniaty; Triani Hardiyati; Agus Hery Susanto; Endang Srimurni Kusmintarsih
BERITA BIOLOGI Vol 21, No 2 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i2.4208

Abstract

Pandanus tectorius Park., tree of life, which almost all of its parts are used by humans. Industrial fiber made from screw pine leaves has been successfully exported to several countries, while the fruit is consumed as an alternative staple food. Fruit is only produced by female plants, because screw pine is dioecious plant. Farmers obtain screw pine seedling using stem cuttings from female trees, but the number of seedling produced is limited and this technique can damage the plant. Alternative seedling production using seeds has constraints in the form of uncertainty over the sex of the seedling. One of the best ways to provide screw pine seedling is to use a shoot-tip culture technique. Shoot-tip culture is able to reproduce plants efficiently and quickly and does not damage the plant. Seedling will also have the same genetic traits as the selected plant. However, efforts to develop an efficient and fast screw pine shoot-tip culture protocol have not been carried out massively. The induction and shoot multiplication stages still had a low success rate. Another problem in seedling production through tissue culture is the certainty seedlings are true-to-type. This review article aims to reveal the potential of shoot-tip culture for in vitro screw pine seedling production and to test the genetic stability of the screw pine seedling.  
Co-Authors AGUS HERY SUSANTO Alice Yuniaty Alvira Rifdah Sativa Daniel Joko Wahyono Dwi Iva Fitriana Ikhsan - Matondang Priskila Agnesia Prayitno Sisunandar . triani hardiyati Trisnowati Budi Ambarningrum