Article Per Year (5 Year)
p-Index From 2019 - 2024
0.444
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat)
VALENTINA YURINA
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
The Role of the First 14 Amino Acids of Mature M1 Protein of Streptococcus pyogenes on Fibronectin-Binding Activity and Dimer Formation ROGA FLORIDA KEMBAREN; ADAM REZA GANJARA; VALENTINA YURINA; DEBBIE SOEFIE RETNONINGRUM
Microbiology Indonesia Vol. 4 No. 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1672.376 KB) | DOI: 10.5454/mi.4.1.5

Abstract

Streptococcus pyogenes is one of the most important human pathogens which express a multi-facet of virulence factors on its cell surface. One of the virulence factors that has been intensively-studied is the M protein that binds several human proteins. M1 protein, a member of the M protein family, was previously found to bind human fibronectin (Fn), an activity that is responsible for bacterial internalization. A structural study showed that this protein consists of four regions: A, B, S, and C. The study was intended to investigate the role of the first 14 amino acid residues located at the non-helical region of M1 protein in binding Fn, and its ability to form a dimer. The DNA fragment encoding for the ABS protein lacking its first 14 amino acids (ABSD14aa) was cloned into pET-16b, overexpressed in Escherichia coli BL21(DE3), and the protein was purified by affinity chromatography. The purified protein was characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis and the Fn-binding activtiy was assayed by enzyme linked immunosorbent assay. The result indicated that the M1 lacking its first 14 amino acids retains its dimerization and Fn-binding activities.
Penguatan Kelompok Kader dan Produksi Produk Sabun dan Hand sanitizer Homemade di Dusun Gondang, Singosari Oktavia Eka Puspita; Oktavia Rahayu Adianingsih; Bachtiar Rifai Pratita Ihsan; Valentina Yurina; Tamara Gusti Ebtavanny
TRI DHARMA MANDIRI: Diseminasi dan Hilirisasi Riset kepada Masyarakat (Jurnal Pengabdian kepada Masyarakat) Vol 2, No 1 (2022)
Publisher : JTRIDHARMA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2022.002.01.30

Abstract

Kegiatan mencuci tangan dengan air mengalir dan sabun atau menggunakan hand sanitizer berbasis alkohol merupakan bagian dari protokol kesehatan sebagai upaya yang dilakukan untuk mencegah penyebaran virus penyebab COVID-19. Untuk menanggapi kebutuhan masyarakat mengenai ketersediaan sabun dan hand sanitizer, maka dilakukan kegiatan pengabdian kepada masyarakat yang bertujuan untuk meningkatkan kemampuan masyarakat dalam memproduksi sabun dan hand sanitizer secara mandiri. Kegiatan ini dilakukan selama bulan Juni hingga Oktober 2021 dengan target sasaran yaitu kelompok Karang Taruna Ketup4t dan PKK di Dusun Gondang, Desa Randuagung, Kecamatan Singosari, Kabupaten Malang, Jawa Timur. Metode pelaksanaan kegiatan adalah participation action research dengan cara pemberian edukasi pembuatan produk dan pendampingan anggota kelompok kader dalam proses pembuatan produk. Hasil utama kegiatan ini adalah kader mampu memproduksi sabun dan hand sanitizer homemade. Kesimpulan dari kegiatan ini adalah Kelompok Kader di Dusun Gondang mampu memproduksi sabun dan hand sanitizer homemade.
Prolonged-heated High-Fat Diet Increase the Serum LDL Cholesterol Level and Induce the Early Atherosclerotic Plaque Development in Wistar Rats Valentina Yurina; Ema Pristi Yunita; Tri Yudani Mardining Raras; Achmad Rudijanto; Kusworini Handono
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Rats are one of the most widely used animal models in health research. However, since rats are relatively resistant to atherosclerosis, the transgenic rats often used to study atherosclerosis in rats. Our study suggests a prolonged-heated lipid to induce atherosclerosis in rats, therefore provide a more low-cost option to study atherosclerosis in rats. Aims of this study is to compare the effect of heated high-fat diet (HFD) to the LDL and HDL cholesterol level and foam cell formation in the Wistar rat animal model. Rats were divided into three groups, control group was given with the normal diet, and the other two treated groups received oxidized HFD (heated HFD) and HFD, respectively. The heated HFD contain lard that was previously heated at 190°C for 24 h. Diet was given for 8 weeks. The serum LDL and HDL cholesterol level were measured before and 4th and 8th weeks after treatment with help of colorimetric measurement. The aorta analyzed for the foam cell formation after HE staining using the light microscope.  The prolonged- heated HFD significantly increased the LDL cholesterol from the 4th week of the treatment (p = 0.023). The rats aortic from HFD and heated HFD treated groups showed a mononuclear cells infiltration and the early foam cell formation. The heating process of the lipid caused lipid oxidation which significantly increased the LDL cholesterol level of the animal model and foam cells formation. This study suggests that Wistar rats with heated HFD could demonstrated early atherosclerosis plaque development.
Co-Authors Achmad Rudijanto ADAM REZA GANJARA Bachtiar Rifai Pratita Ihsan DEBBIE SOEFIE RETNONINGRUM Ema Pristi Yunita Kusworini Handono Oktavia Eka Puspita Oktavia Rahayu Adianingsih ROGA FLORIDA KEMBAREN Tamara Gusti Ebtavanny Tri Yudani Mardining Raras