Garuda - Garba Rujukan Digital

ADELINA MANURUNG

Unknown Affiliation

Author-ID : 3152987

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Antioxidant and Antibacterial Activities Enhancement of Solid-state Fermented Candlenut Kernels by Aspergillus oryzae GRACE DOLOROSA LIMBONG; LEVY NATHANAEL NABABAN; ADELINA MANURUNG; MERRY MERYAM MARTGRITA
Microbiology Indonesia Vol. 13 No. 2 (2019): June 2019
Publisher : Indonesian Society for microbiology

According to several studies, solid state fermentation (SSF) can enhance antioxidant and antibacterial activity of natural sources, and microorganism that is widely used in this kind of research is Aspergillusoryzae. Therefore, this study employed SSF by A. oryzae to enhance antioxidant and antibacterial activity of candlenut kernel. Candlenut kernel powder, that has been moistened with 60% water, was inoculated with 10% (w/w) of 5-day-culture ofA. oryzae, and was fermented for 9 days (until exponential phase; sample-1)and 12 days(until stationary phase; sample-2). The fermented candlenut kernels was extracted by ethanol and concentrated using rotary evaporator. Total phenolic content of control (unfermented extract), sample-1, and sample-2 are 0.183 mg GAE/g, 2.761 mg GAE/g, and 4.194 mg GAE/g, respectively. This results supported the IC50 value determined by DPPH method, those are 617.11 Î¼g/ml, 260.23 Î¼g/ml, and 45.29 Î¼g/ml. These results revealed a very strong antioxidant activity (< 50 Î¼g/ml) in the sample fermented until stationary phase.Antibacterial assay to Staphylococcus aureus resulted diameter of inhibition zone 7.17 mm, 13.51 mm, and 18.51 mm, respectively. And to Pseudomonas aeruginosa resulted diameter of inhibition zone 6.52 mm, 11.786 mm, and 15.269 mm, respectively. From this result, SSF until stationary phase enhanced higher antioxidant and antibacterial activity compared the other treatments.Â

Citric Acid Production From Toba Banana Peel (Musa acuminata Colla) Through Submerged Fermentation Using Aspergillus niger MEVA GUSTINA E. SIDAURUK; SURYA NINGSIH HUTAURUK; MERRY MERYAM MARTGRITA; ADELINA MANURUNG
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Toba banana peel waste is derived from Toba banana fruit (Musa acuminata Colla) processing. Local people utilized banana peel waste usually as livestock feed. The waste also can make an environmental problem if it is not handling well. Banana peel waste has a high content of carbohydrate that can be fermented to produce a more valuable product, one of which is citric acid. Citric acid is an organic acid that is consumed globally and produced in large quantities. In food and beverages industries, citric acid is used for various purposes due to its high solubility, non-toxic and good taste characteristics. The objective of this research is to determine the optimum conditions of submerged fermentation of banana peel to produce citric acid using Aspergillus niger. The treatments were various banana peel concentrations (5%, 10% and 15% w/v) added with 5% sucrose or 5% glucose (w/v). During the fermentation, pH was measured to determine pH changes indicated the production of citric acid. The results showed that the variation concentration of banana peel substrate and type of sugars affect citric acid production. The optimum condition of submerged fermentation by Aspergillus niger was obtained at 15% substrate concentration by adding 5% sucrose to produce 0.651% (w/v) of citric acid.

The Effect Of Aeration Rate On The Growth Of Blue Green Microalgae in Buffalo Dung As Alternative Media EDWIN YONATHAN GURNING GURNING; AMOS IMANUEL; NINA JULIANA ROBERTA TURNIP; ADELINA MANURUNG
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

The high demand of Arthrospiraplatensis as a veritable protein source encourages its mass production worldwide. Currently, mass production of Arthrospiraplatensis is hindered by the relatively high price of the growth media. Recently, it is discovered that Arthrospiraplatensis can be cultivated using buffalo dung as an alternative medium. Buffalo dung is an excellent source of nitrogen and phosphorus which are principal macronutrients for the growth of Arthospiraplatensis. In addition to nitrogen and phosphorus, carbon is also a macronutrient that is important to the growth of microalgae. The carbon source used by the microalgae is carbon dioxide, which is consumed through photosynthesis. Carbon dioxide can be derived directly from the atmosphere as atmospheric CO2 existing as much as 0.04%-v/v in air, which can be provided directly using an aeration pump into the growth medium microalgae. During the aeration process, CO2 mass transfer occurs from the gaseous phase into the liquid phase. This research aims to investigate the effect of the aeration rate on the growth of the blue-green microalgae Arthrospiraplatensisusing buffalo dung media as an alternative medium. Arthrospiraplatensis will be cultivated on buffalo dung media using various aeration rates to determine the effect of aeration on the specific growth rate (Âµ). The air will also be pumped into the growth medium without Arthrospiraplatensis at the specific aeration rates to determine the mass transfer coefficient (kLa) that occurs from the air leading to growth medium. Analysis of mass transfer coefficient (kLa) of carbon dioxide will be conducted using the sulfite method. Variation of aeration that used in this research are 0.2 vvm; 0.4 vvm; 0.6 vvm; 1.2 vvm; 2.4 vvm that has mass transfer coefficient dan specific growth rate are 0.005 min-1 and 0.1987 day-1; 0.009 min-1 and 0.2279 day-1; 0.012 min-1 and 0.2044 day-1; 0.034 min-1 and 0.1918 day-1; 0.035 min-1 and Âµ in 2.4 vvm canâ€™t determine, respectively.

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