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EKSTRAKSI DNA DAN AMPLIFIKASI ITS rDNA ISOLAT FUNGI ENDOFIT LBKURCC67 UMBI TANAMAN DAHLIA (DAHLIA VARIABILIS) Senjavi Rakhmana; Saryono '; Titania Tjandrawati Nugroho
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

> Endophyte fungi lives in the plant tissues without causing harm to their host and has known that can produce secondary metabolite and extracellular enzyme. Fungi LBKURCC67 is an endophyte fungi that was isolated from tubers of yellow flowered Dahlia variabilis in Padang Panjang, West Sumatera. Species of fungi LBKURCC67 isolate was not known exactly because the morphology identification has been previously is appropriate for genus level. The accurate identification to known species is molecular with phylogenetic analysis. Before determine species in molecular analysis, it must extraction and amplification DNA. This research aims to optimation of DNA extraction and rDNA amplification in Internal Transcribed Spacer (ITS) regions with Polymerase Chain Reactions (PCR) methods. The DNA of fungi LBKURCC67 isolate was extract with Wizard Genomic DNA Purification kit ex Promega Corp. (Madison, USA) from cultur mycelium. The result shows extraction DNA was success from fourth days mycelia. Optimum condition for rDNA amplification with PCR were used ITS5 and ITS4 primers and 41°C annealing temperature. Electrophoresis analysis shows molecular weight of DNA isolate is 16.951 bp and molecular weight of PCR product is 583 bp.
STUDI PRODUKSI ASAM LEVULINAT DARI PATI UBI GAJAH (Manihot esculenta) MENGGUNAKAN KATALIS ASAM SULFAT Wisnu Aditya; Amir Awaluddin; Saryono '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The utilization  of  Manihot esculenta  to produce  platform chemical such as    levulinic acid  (LA)    has  been  studied    in  detail to  optimize    the  LA  production. The  LA production from  Manihot esculenta  was  monitored  using    reaction times  of  the hidrolysis reaction (3-85 min.),    the reaction temperatures  (150˚C, 170˚C,  and 190˚C), and  concentrations  of sulfuric acid as the catalyst  (1%,  3%,  and 5%).  The  products obtained  were  then  characterized using  high performance  liquid  chromatography (HPLC)    to determine the contents of gl ucose,  hydroxymethylfurfural  (HMF)    and LA. The result showed that  the LA production increased with  the  rise    of temperature  and catalyst concentration, whereas the    glucose and HMF  production decreased with    the rise  of temperature and catalyst concentration. The highest  production    of LA reached approximately 50% (based  on initial concentration of biomass) at reaction temperature of 170˚C and 5% of sulfuric acid concentration for 70 minutes of reaction time.
ISOLASI DNA DAN AMPLIFIKASI PCR DAERAH ITS rDNA FUNGI ENDOFIT UMBI TANAMAN DAHLIA (Dahlia variabilis) LBKURCC69 Fitri Rahayu; Saryono '; Titania Tjandrawati Nugroho
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Endophytic fungi lives within healthy plant tissues without causing damage to the host plant. Endophytic fungi LBKURCC69 was one of the endophytic fungi that was isolated from the tubers of dahlia plant (Dahlia variabilis). Morphological identification showed that endophytic fungi LBKURCC69 was Phialophora fastigiata. This identification can’t provide an accurate result because many species of fungi with the same morphological features, that causing misidentification. More accurate species identification can be done by molecular identification using rDNA ITS region. Electrophoresis results indicated that chromosomal DNA of endophytic fungi LBKURCC69 was successfully isolated and has a molecular weight of 6124 bp. DNA amplification of endophytic fungi LBKURCC69 on rDNA ITS regions was successfully performed using ITS4 and ITS5 primers with 45°C annealing temperature and produces DNA fragments with a molecular weight of 537 bp.
OPTIMALISASI pH PRODUKSI ENZIM SELULASE DARI BAKTERI ENDOFITIK Pseudomonas stutzeri LBKURCC45, Pseudomonas cepacia LBKURCC48 DAN Pseudomonas stutzeri LBKURCC59 Ajaib Prima; Silvera Devi; Saryono '
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The isolate of LBKURCC45, LBKURCC48 and LBKURCC59 are endophytic bacteria that have been isolated from the tubers of dahlia. Endophytic bacteria can get into the plant tissue through injured plant tissue or because the bacteria can produce cellulase to degrade plant cell wall that contain cellulose. Cellulase is an enzyme that can hydrolyze the β-1-4-glycosidic bond of cellulose. This study was carried out to determine the optimum pH of cellulase enzyme production (6.0; 6.5; 7.0; 7.5; and 8.0). Enzyme activity was calculated based on the amount of reducing sugar formed from Carboxymethyl cellulose (CMC) substrate hydrolyzed by cellulase enzyme with Nelson-somogyi method. The result showed that the highest activity of cellulase enzyme obtained at pH 7 at 24 hours production time. The cellulase activity of LBKURCC45, LBKURCC48, and LBKURCC59 was 0.415 ± 0.043 x 10-3 U/mL, 0.353 ± 0.069 x 10-3 U/mL, and 0.246 ± 0.050 x 10-3 U/mL, respectively.