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All Journal Jurnal Penelitian Tanaman Industri REINWARDTIA Biosaintifika: Journal of Biology & Biology Education
RITA MEGIA
Jurusan Biologi FMIPA Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Education Industrial & Manufacturing Engineering

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Journal : Biosaintifika: Journal of Biology

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In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood Nurhayani, Siti; Megia, Rita; Purnamaningsih, Ragapadmi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.11079

Abstract

A diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile axillary shoot. The explants were planted on MS0 medium for 2 weeks and later on multiplication medium MS+0.3 mg/l BAP + 0.3mg/l TDZ. The shoots obtained were fragmented into clusters (3-5 shoots) used for the next multiplication stage using five different medium formulas: (1) MS0; MS containing: (2) 0.1 mg/l BAP, (3) 0.3 mg/l BAP, (4) 0.1 mg/l BAP + 0.1 mg/l TDZ and (5) 0.3 mg/l BAP + 0.1 mg/l TDZ. The results showed that MS medium containing 0.1 mg/l BAP + 0.1mg/l TDZ was the best medium for B. balcooa propagation. The shoots produced from aforementioned medium had a better quality compared to the other medium. Forty days after planting, the average number of shoots in this medium was 14.25. MS medium containing 0.3 mg/l BAP + 0.1 mg/l TDZ produced the highest number of shoot but in lower quality. Rooting medium containing 10 mg/l IBA + 5 mg/l NAA produced 9-16 root in 8 weeks. Vermicompost was more prevalent for the acclimatization of B. balcooa compared to compost. The use of B.balcooa resulted in in vitro propagation as a substitute alternative for wood is expected to save the environment from illegal logging.  
Co-Authors . HADISUNARSO, . . Ratnasari ALEX HARTANA Ary Prihardhyanto Keim, Ary Prihardhyanto HADISUNARSO ., HADISUNARSO Ika Roostika Ireng Darwati Nurhaidah Iriany Sinaga, Nurhaidah Iriany Nurhayani, Siti RAGAPADMI PURNAMANINGSIH RATNASARI ., RATNASARI