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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika
Suharjono Suharjono
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Diversitas dan Uji Potensi Bakteri Kitinolitik dari Limbah Udang Imanda Nurul Setia; Suharjono Suharjono
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : University of Brawijaya

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Abstract

ABSTRAK Proses pengolahan udang dari industri akan menghasilkan limbah cair dan padat sebanyak 30 – 75 % dari berat udang. Limbah udang mengandung 20 – 60 % kitin sehingga dapat menjadi sumber bakteri kitinolitik. Tujuan penelitian ini adalah untuk mengetahui diversitas bakteri kitinolitik dan mengetahui potensi bakteri yang memiliki aktivitas kitinolitik yang tinggi pada limbah udang. Tahapan penelitian meliputi isolasi bakteri pada media CCA (Colloidal Chitin Agar), penghitungan diversitas bakteri kitinolitik, dan seleksi isolat berdasarkan indeks kitinolitik. Diversitas bakteri kitinolitik dihitung menggunakan indeks diversitas Simpson. Penelitian uji potensi kitinolitik menggunakan Rancangan Acak Lengkap (RAL) dengan tiga kali ulangan. Data indeks kitinolitik dianalisis menggunakan analisis ragam dilanjutkan uji Games-Howell (α = 0,05). Indeks diversitas limbah padat lebih tinggi (0,646) daripada limbah cair (0,213). Dua dari 18 isolat yaitu isolat PBK 2 (berasal dari limbah cair) dan SA 1.2 (berasal dari limbah padat) memiliki indeks kitinolitik tertinggi yaitu secara berturut-turut sebesar 2,069 dan 2,084. Kata kunci : bakteri kitinolitik, limbah cair, limbah padat, kitin, udang ABSTRACT Shrimp processing from industrial activity produce solid waste and wastewater 30 – 75 % from shrimp weight. Shrimp waste contains 20 – 60 % chitin and possible to be source of chitinolytic bacteria. The objectives of this research was to observe diversity of chitinolytic bacteria and to analyze potency of bacteria which have high activity of chitin degradation in shrimp waste. The research consist of isolation of bacteria using Colloidal Chitin Agar (CCA) medium, quantification of chitinolytic bacteria diversity, and screening of bacteria based on chitinolytic index. Diversity of chitinolytic bacteria quantified with Simpson diversity index. Chitinase assay carried out according Complete Randomized Design with three replications. Diameter of clear zone data was analyzed using analysis of variance continued with Games-Howel test (α = 0.05). Diversity index of solid waste more higher (0.646) than wastewater (0.213). Only two of eighteen isolates of chitinolytic bacteria (PBK 2 and SA 1.2 isolates) showed highest chitinolytic index. Key words: chitinolytic bacteria, chitin, shrimp, waste
Cyanobacteria Community Dynamics and Trophic Status of Intensive Shrimp (Litopenaeus vannamei) Farming Pond in Situbondo East Java Indonesia Dian Aliviyanti; Suharjono Suharjono; Catur Retnaningdyah
Journal of Tropical Life Science Vol. 7 No. 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.10

Abstract

The objective of this study was to analyze the dynamics community structure of Cyanobacteria and trophic status in ponds of intensive shrimp culture (Litopenaeus vannamei) in Situbondo based on Trophic Diatom Index (TDI).  The ex post facto research was conducted in situ in the hamlet of Pond Mutiara Mas III Klatakan Situbondo East Java Indonesia.  Observation of Cyanobacteria and Diatoms community structure were done every week during four cycles of shrimp farming ponds.  Cycle of shrimp farming ponds is the times for enlargement process of the shrimps from seed to mature which ranges from 90-120 days.  The dependent variables were the density of Cyanobacteria and Diatoms community, as well as chemical parameters nitrite and orthophosphate.  Trophic status was determined from TDI be counted from data of diatom density.  All of the data were then classified using cluster and biplot analysis program PAST Ver. 3.11 to discribe the profile of ecosystem quality.  The research results showed that there were four taxa of Cyanobacteria during farming cycle which the highest density was found from the genus of Oscillatoria.  Based on the value of TDI, we found that the trophic status of water in shrimps pond during the production process was eutrophic until hyper-eutrophic.  The water quality was decreases along with the length of incubation time.
The Impact of Dissolved Nitrate and Phosphate on Maximum Growth Rate and Carrying Capacity of Oscillatoria in Intensive Shrimp (Litopenaeus vannamei) Farming Pond Situbondo, East Java, Indonesia Dian Aliviyanti; Suharjono Suharjono; Catur Retnaningdyah
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1219.817 KB) | DOI: 10.21776/ub.jels.2016.007.01.11

Abstract

The aims of study are to analyze the effect of dissolved nitrate and phosphate content of the intensive shrimp farming pond Situbondo to maximum growth rate and carrying capacity of Oscillatoria population density in the laboratory. This is an experimental research method using completely randomized design with three replications. The treatment were variation of nitrate and phosphate concentration (N0; N6; N12; N24; N48; P0,2; P0,4; P0.8, P1.6  mg.L-1).  Experiment was done using a pure Oscillatoria culture in condition 25 watt lamp; 12 hours a day. The initial amount of Oscillatoria cells used for the treatment is 8 - 15 x 104 cell.mL-1. During the incubation process, chemical parameters were also observed including nitrate, phosphate, DO, and pH at the beginning of the incubation period. Oscillatoria cell was count every day until stationary phase for 30 days. Furthermore, a different test between treatments was conducted to determine levels of nitrate and phosphate in triggering the blooming of Oscillatoria using Oneway ANOVA analysis with SPSS Program. The results showed that the intensive shrimp pond waters of Situbondo already contain dissolved phosphate between 0.4 - 0.5 mg.L-1. Oscillatoria growth is strongly influenced by dissolved phosphate content in waters, phosphate levels of 0.2 - 0.4 or equivalent to the actual level of 0.6 - 0.7 mg.L-1 can cause the highest abundance of Oscillatoria that could endanger the ecosystem.  Keywords: dissolved phosphate, intensive shrimp farming, Nitrate, Oscillatoria.
Plant Growth Promoting Endophytic Bacteria of Coffea canephora and Coffea arabica L. in UB Forest Esti Rizkiana Pratiwi; Tri Ardyati; Suharjono Suharjono
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.07

Abstract

Plant Growth Promoting (PGP) Endophytic bacteria are used as an alternative biofertilizer to support soil health and plant productivity. This research aimed to isolate, analyze the potential, and identify the endophytic bacteria of Robusta and Arabica coffee plants as biofertilizer agents. Endophytic bacteria were isolated from the roots of coffee plants and tested for their potential to produce IAA, phosphate-solubilizing, and nitrogen fixation. Potential endophytic bacterial isolates were identified based on 16S rDNA sequence similarity. Total isolates from Robusta coffee consisting of ten IAA-producing bacteria, eight phosphate-solubilizing, and seven nitrogen fixation bacteria isolates. Total isolates from Arabica coffee roots were 12 isolates of IAA-producing bacteria, seven isolates of phosphate-solubilizing bacteria, and six isolates of nitrogen fixation bacteria. The highest potential of the isolate from Robusta roots was SS.E2 isolate to produce IAA 110.73 μg.mL-1; SS.P3 isolate to dissolve phosphate 4.42 μg.mL-1, and SS.N2 isolate to produce ammonium 3.15 μg.mL-1. The highest potential of the isolate from Arabica roots was SW.E9 isolate to produce IAA up to 257.16 μg.mL-1; SW.P5 isolate to dissolve phosphate up to 4.55 μg.mL-1; and SW.N6 isolate to produce ammonium up to 1.16 μg.mL-1. Isolates SS.E2, SW.E9, SS.P3, SW.P5, SS.N2, and SW.N6 were respectively identified as Bacillus cereus ATCC 14579, Bacillus cereus ATCC 14579, Rahnella aquatilis B35, Kluyvera intermedia TPY16, Rahnella aquatilis B35, and Pseudomonas tolaasii NCPPB 2192. Potential PGP isolates can be developed as biofertilizer agents for the coffee plant. Keywords: Coffee, Endophytic bacteria, IAA, Nitrogen, Phosphate
Co-Authors Catur Retnaningdyah Dian Aliviyanti Esti Rizkiana Pratiwi Imanda Nurul Setia Tri Ardyati