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Muchamad Luthfi
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The success pregnancy rate with estrous synchronization hormones in Madura cow Muchamad Luthfi; Jauhari Efendy
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 3 (2019): Desember
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.03.02

Abstract

This study aimed to determine the efficiency and effectiveness of the use of PGF2α and ovsynch (PGF2α and GnRH) estrous synchronization hormones on their ability to have an impact in the form of estrous and pregnancy symptoms in Madura cow. The material used were 182 Madura cows, was consisted by three treatments, namely A treatment with 62 cows; first day (H-0) was injected with PGF2α dose of 2 ml; 11th day (H-11) injected with the same hormone and dosage; then 3-4 days after the second AI (Artificial Insemination) injection directly; treatment B of 60 cows; first day (H-0) injected PGF2α dose 3 ml; 11th day (H-11) is injected again with the same hormone and dosage; then 3-4 days after injection of the second hormone directly AI; C treatments were 60 cows; given ovsynch treatment; that is, on the first day (H-0) injected with a 2.5 ml dose of the GnRH hormone; 8th day (H-8) injected with PGF2α dose 2 ml; the 10th day was injected again with the second GnRH hormone (2.5 ml dose), then the 11th day was directly AI. Sampling was done by purposive sampling method, which was choosing a cow that has a body condition score (BCS) between 5-7, normal reproductive organs and not in a state of pregnancy. The study design used a completely randomized design; Data were analyzed using the Kruskal Wallis test. The results showed that the administration of synchronous hormones to the percentage of estrous and pregnancy rate in all treatments showed no significantly different results (P > 0.05). The average percentage of estrous Madura cows in all treatments was 94.50%, while the average pregnancy rate was 68.66%, the highest yield in treatment C was 73.33%; followed by treatments A and B respectively 70.97% and 61.67%.
Comparative study of sperms motility analysis with CASA by using leja and microscope slide Dian Ratnawati; Muchamad Luthfi
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 30, No 2 (2020): August 2020
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2020.030.02.03

Abstract

The aim of this study was to compare the assessment of spermatozoa motility by using leja and microscope slide (MS). Fresh semen of four Ongole crossbred (PO) bulls were collected by using an artificial vagina. The semen with 70% sperms motility was diluted into into 30 million/ml concentration and assessed for its motility by using CASA (SCA 5.2 Microptic). The measured parameters in this study were sperms motility, progressive sperm motility, curve linear velocity (VCL), straight linear velocity (VSL), average pathway velocity (VAP), linearity (LIN), straightness (STR), wobble (WOB), hyperactivity (H), lateral head amplitude (ALH) and beat cross frequency (BCF). The data were analyzed by using independent simple t-test (IBM SPSS statistics 24). The results showed a hyperactive spermatozoa when analyzed by using leja was at 7.3%, higher than assessed with Microscope slide (2.8%). Meanwhile, other motility parameters (motility, progressive motility, VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) did not show any difference. Microscope slides can be used as a means of supporting sperms motility assesment by using CASA and more efficient.
Co-Authors Dian Ratnawati Jauhari Efendy