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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Research Journal of Life Science ANNALES BOGORIENSES
Swastika Praharyawan
Research Center for Biotechnology, Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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Influence of Light Intensity on Lipid Productivity and Fatty Acids Profile of Choricystis sp. LBB13-AL045 for Biodiesel Production Swastika Praharyawan; Delicia Yunita Rahman; Dwi Susilaningsih
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.5 KB) | DOI: 10.21776/ub.rjls.2018.005.02.7

Abstract

The influence of various light intensity on the lipid productivity, fatty acids profile, and biodiesel properties of Choricystis sp. AL045 were examined. Choricystis sp. LBB13-AL045 was a high lipid content microalgal strain (up to 42.49 ± 0.41% per dry weight basis) with great performance of its growth (specific growth rate and biomass productivity were up to 0.802 ± 0.013 day-1 and 108.57 ± 8.07 mg L-1 day-1, respectively). Such results indicated its high lipid productivity and its potency to be used for biodiesel production. The treatment of various light intensities on the microalgal culture resulted obvious differences in lipid productivity and fatty acids composition. Maximum lipid productivity (46.13 ± 3.43 mg L-1 day-1) was at 405 µmol m-2 s-1 of light intensity. The fatty acids profiles of four light intensities treatments were quite similar; the major components of fatty acid obtained from Choricystis sp. LBB13-AL045 oil were oleic acid (C18:1) and palmitic acid (C16:0), which provided a strong indication of high-quality biodiesel. Biodiesel properties were determined by empirical equations and found to be within the limits of biodiesel standard SNI 7182:2015, ASTMD6751-08 and EN14214.
Evaluation of Alternative Components in Growth Media of Lactobacillus brevis for Halal Probiotic Preparation Riyona Desvy Pratiwi; Sabighoh Zanjabila; Dian Fairuza; Aminah Aminah; Swastika Praharyawan; Asrul Muhamad Fuad
ANNALES BOGORIENSES Vol 24, No 1 (2020): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2020.v24.n1.11-17

Abstract

Probiotic has been widely used in functional food because of numerous advantages for health. MRS broth is commonly used as standard medium in studying lactobacilli. However, in some communities - like muslim and vegetarian society, components in MRS broth/medium become an issue. Beef extract and peptone – animal derived substances as nitrogen sources in the MRS medium should be avoided for the vegetarian. Meanwhile, for the muslim society, all components must be halal-certified including those animal derived ingredients. Therefore, several alternative sources for beef extract and peptone substitution were studied. Combination of alternative nitrogen sources was applied. In order to increase the effect of the alternative nitrogen sources, alternative carbon sources were also included. This is the first report about effects of L. brevis media components on cells growth to expression level of surface layer protein (Slp). Whey, lactose, sucrose, and galactose showed high contribution to L. brevis growth. However, the tested concentration of those substances were not sufficient to obtain equal bacterial growth and Slp expression than that of MRS broth. In addition, yeast extract appeared necessary to maintain cell wall and Slp expression.
Characterization of lipid productivity and fatty acid profile of three fast-growing microalgae isolated from Bengkulu for possible use in health application Swastika Praharyawan; Delicia Yunita Rahman; Dwi Susilaningsih
Journal of Tropical Life Science Vol. 6 No. 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.03

Abstract

Three strains of fast-growing microalgae were investigated in this study for their potential of lipid production and its possible use in health application. Lipid content, lipid productivity, and fatty acids profile of the 3 mi-croalgae strains were also analyzed. The strain of LBB13-2-AL045 and LBB13-2-AL048 possessed highest lipid content (49.08 ± 0.25%) and lipid productivity (40.27 ± 1.91 mg.L-1.day-1), respectively, among the other tested strains. The fatty acids profile from the 3 strains exhibited its possible use in health application. The two strains of LBB13-2-AL046 and LBB13-2-AL048 possessed high valuable fatty acids of polyunsaturated fatty ac-ids (PUFA) omega-3 and omega-6, whereas LBB13-2- AL045 possessed balance ratio of saturated fatty acid (SFA) : monounsaturated fatty acid (MUFA) : PUFA (1 : 1.3 : 1) as is recommended by American Heart As-sociation (AHA) (1 : 1.4 : 0.8). The high content of omega-3 and omega-6 fatty acids indicated that the algal lipids of two strains (LBB13-2-AL046 and LBB13-2-AL048) were potential to be applied in cardiovascular health. The balance ratio of SFA : MUFA: PUFA as is recommended by AHA indicated that the algal oil of LBB13-2-AL045 strain was recommended in order to generate the best LDL/HDL ratio.
Co-Authors Aminah Aminah Asrul Muhamad Fuad Delicia Yunita Rahman Delicia Yunita Rahman Dian Fairuza Dwi Susilaningsih Dwi Susilaningsih Riyona Desvy Pratiwi Sabighoh Zanjabila