Pipin Suciati
Program Studi Akuakultur, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.

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Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik [Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics] Wahju Tjahjaningsih; Endang Dewi Masithah; Heru Pramono; Pipin Suciati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1343.957 KB) | DOI: 10.20473/jipk.v8i2.11182

Abstract

Abstrak Probiotik dapat didefinisikan sebagai mikroba hidup yang ditambahkan dalam jumlah tertentu yang mampu bertahan hidup dalam ekosistem saluran pencernaan. Enzim yang dihasilkan oleh mikroba yang diisolasi dari saluran pencernaan ikan dapat digunakan sebagai probiotik. Enzim proteolitik ekstraseluler secara alami diproduksi oleh mikroba untuk menghidrolisis polipeptida dalam media menjadi peptida dan asam amino. Bakteri asam laktat dapat menghasil enzim seperti protease, α-amilase, fitase, kitinase, lipase. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.) yang mempunyai aktivitas proteolitik. Penelitian ini menggunakan metode deskriptif berupa aktivitas enzimatis dan karakterisasi uji biokimia isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.). Jumlah kepiting bakau yang digunakan adalah 10 ekor. Hasil penelitian didapatkan tiga isolat bakteri asam laktat yang memiliki aktivitas proteolitik kuat, yaitu WK 28, WK 33, dan WK 53. Hasil uji biokimia isolat WK 28 termasuk ke dalam genus Pediococcus sp., isolat WK 33 termasuk ke dalam Lactobacillus sp., dan isolat WK 53 termasuk ke dalam genus Streptococcus sp. WK 28 (Pediococcus sp.) dan WK 33 (Lactobacillus sp.) mempunyai aktivitas proteolitik dan aktivitas lipolitik. Isolat WK 53 (Streptococcus sp.) mempunyai aktivitas enzimatis yaitu aktivitas proteolitik, amilolitik, dan lipolitik. Abstract Probiotics are defined as live microbes are added in a certain amount that is able to survive in the digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can be used as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze a polypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such as proteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolates from the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This research uses descriptive method such as enzymatic activity and biochemical characterization of isolates of lactic acid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish. The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK 28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genus Pediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to the genus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolytic activity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolytic activity, amylolytic, and lipolytic.
Eksplorasi Rafinosa Biji Kapas sebagai Pengganti Formalin dalam Pengawetan Ikan [Raffinose Exploration Cotton Seeds Lieu Of Formalin In Preserving Fish] Abdul Manan; Khairanita K; Pipin Suciati; Kurnia Ayu K.W; Moch. Amin Alamsjah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.854 KB) | DOI: 10.20473/jipk.v5i2.11399

Abstract

Abstract Preservation is a human effort to enhance durability and shelf life of the fish that the quality of fish remains in good condition. Irregularities in the use of formalin preservation would endanger the health of consumers. Cottonseed is containing compounds of Raffinose, proteins, fats that are cryoprotectants to cells and antimicrobe, that use of cottonseed to suppress use of formalin as a preservative of fish in the community. The method used by dissolving cottonseeds in organic solvents, where in the organic solvent effective to extract the active ingredients in cottonseed. The results showed use of cotton seed extract is 100% equal to 100% use of formalin in maintaining the quality of the fish. This is evidenced by organoleptic tests, pH and bacterial tests. Expected with these results, cottonseed can be a substitute for use of formalin.