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All Journal Folia Medica Indonesiana Biomolecular and Health Science Journal
Budiono Budiono
Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Other

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Comparation of Phenotypic and Genotypic Profile of Carbapenemase Producing Escherichia coli Silvia Sutandhio; Budiono Budiono; Hardiono Hardiono; Kuntaman Kuntaman; Eddy Bagus Wasito; Maria Inge Lusida
Folia Medica Indonesiana Vol. 54 No. 1 (2018): March
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.592 KB) | DOI: 10.20473/fmi.v54i1.8045

Abstract

Carbapenemase-producing Escherichia coli (E. coli) has caused trouble in therapeutic antibiotic selection. Carbapenemase screening procedure in laboratories is usually based on inacurate semi-automatic system. Confirmation and classification of carbapenemases according to Ambler can be done with combination of phenotypic methods, i.e., Modified Hodge Test (MHT), Sodium Mercaptoacetic Acid (SMA), and 3-Aminophenylboronic Acid (PBA). This study aimed to compare profiles of carbapenemase-producing E. coli which were confirmed and classified phenotypically with the genotypic profiles. E. coli isolates from urine specimens which were potential as carbapenemase-producers according to semi-automatic system BD Phoenix were phenotypically tested with MHT, SMA, and PBA. Isolates were grouped as carbapenemase-producers and non carbapenemase-producers. Phenotypic carbapenemase-producer isolates were classified based on Ambler criteria. All isolates were then tested with Polymerase Chain Reaction (PCR) for the presence of OXA-48, IMP1, IMP2, GES, VIM, NDM, KPC genes. Out of 30 isolates, 6 isolates (20.0%) were MHT positive, and 25 isolates (83.3%) were SMA positive, which indicated that most isolates produced were carbapenemase Ambler B. PCR confirmed 12 isolates (40.0%) had VIM gene which were classified as carbapenemase Ambler B. Phenotypic confirmatory test had 100% sensitivity and 22.2% specificity. Classification with phenotypic confirmatory test had 91.7% match with PCR. Phenotypic confirmatory test detected more carbapenemase than PCR. This low specificity may be caused by inappropriate use of diagnostic gold standard. PCR should not be used for routine carbapenemase confirmation because of vast diversity of carbapenemases. Phenotypic confirmatory test can classify carbapenemase according to Ambler classification.
The Comparison of Giardia lamblia Infection and Nutritional Status of Elementary School Students in Mandangin Island, Sampang and Mojo Village, Surabaya, Indonesia Zaidan Arifiansyah Bachtiar; Amalia Putri Uswatun Hasanah; Muhammad Yasin; Rahmadany Isyaputri; Budiono Budiono; Sukmawati Basuki
Biomolecular and Health Science Journal Vol. 3 No. 2 (2020): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v3i2.22406

Abstract

Introduction: Giardia lamblia infection can cause impaired absorption of nutrientsand affects to the nutritional status that has not yet fully investigated in Indonesia.This study aimed to investigate the comparison of prevalence of Giardia lambliainfection and nutritional status of elementary students in Mandangin island and Mojovillage where were different condition.Methods: An analytical observational study with cross sectional design wasconducted in Mandangin island in July 2017 and Mojo village in June 2018 byinformed consent. Stools of elementary students were collected for Giardia lambliaidentification under a light microscope using direct smear method. Nutritional statusof students were determined by body mass index (BMI), and categorized as severeunderweight, underweight, healthyweight, overweight, and obese.Results: Forty-five stools samples were collected from elementary students inMandangin island and 55 stools samples in Mojo village. Prevalence of Giardialamblia infection without symptoms was higher in elementary students in Mandanginisland than Mojo village (28,9%, 13/45 and 1,8%, 1/55, respectively). Giardiasisprevalence in Mandangin island was significantly difference with Mojo village(p<0.0001, chi square-test). Nutritional status of students in both study sites showeddominant healthy-weight that were 73.3% (34/55) in Mandangin island and 61.8%(33/45) in Mojo village. Giardiasis was commonly observed in students with healthy-weight and it was higher in Mandangin island (8/33, 24.2%) than Mojo village (1/34,2.9%).Conclusion: Asymptomatic giardiasis was highly found in elementary students inMandangin island and commonly observed in students with healthy-weight from both 3 regions. It suggested that asymptomatic giardiasis might not affect to the nutrition status of students.
Co-Authors Amalia Putri Uswatun Hasanah Eddy Bagus Wasito Hardiono Hardiono Kuntaman Kuntaman Maria Inge Lusida Muhammad Yasin Rahmadany Isyaputri Silvia Sutandhio Sukmawati Basuki Zaidan Arifiansyah Bachtiar