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All Journal Indonesian Journal of Biotechnology
Fenny M Dwivany
School of Life Science and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung 40132, Indonesia; ForMIND Institute, Bandung, Indonesia; Bali International Research Center for Banana, Universitas Udayana, Bukit Jimbaran, Kuta Selatan,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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Analysis of ethylene biosynthesis gene expression profile during titanium dioxide (TiO2) treatment to develop a new banana postharvest technology Fenny M Dwivany; Rizkita R Esyanti; Veinardi Suendo; Aksarani ‘Sa Pratiwi; Annisa A Putri
Indonesian Journal of Biotechnology Vol 24, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (29.653 KB) | DOI: 10.22146/ijbiotech.51718

Abstract

Banana is an important crop that demands proper methods in postharvest handling. As a climacteric fruit, thebanana fruit ripening process is affected by ethylene. Several methods have been developed to extend the shelf life of a banana, such as using ethylene scrubbers. In this study, ttanium dioxide (TiO2), a photocatalyst, was used as an alternatve method to delay the fruit ripening process. The effect of TiO2 on the ripening‐related gene MaACS1 was investgated. Banana fruits were placed in a TiO2‐coated glass chamber and observed for ten days. Fruit ripening in the treated chamber was delayed for eight days compared to the control. Total RNA was extracted from control and TiO2‐treated fruit pulp and synthesized into cDNA. Reverse transcripton PCR was performed to investgate the gene expression, which showed that MaACS1 expression was relatvely lower than treated control. The fnding of these studies suggested that the TiO2 chamber has the potental to extend the shelf life of banana by delaying its ripening process and decreasing the expression of MaACS1. To the best of our knowledge, no previous study has investgated the effect of TiO2 on the expression of genes related to banana fruit ripening.
Co-Authors Aksarani ‘Sa Pratiwi Annisa A Putri Rizkita R Esyanti Veinardi Suendo