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Raden Taufiq Purna Nugraha
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Variasi dan Filogeni Kancil dan Napu (Tragulus Sp.) di Indonesia Menggunakan Gen 12s rRNA Mitokondria (VARIATION AND PHYLOGENI ON GENUS TRAGULUS (TRAGULUS SP.) IN INDONESIA USING MITOCHONDRIAL 12s rRNA GENE) Wirdateti .; Raden Taufiq Purna Nugraha
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Genus Tragulus in Indonesia is consisted of three species, namely Tragulus javanicus, T. napu, and T.kanchil. These three species are distributed in Kalimantan (Borneo), Java, Sumatra and Lesser Sundaislands. Taxonomically, based on morphological characteristic,T. javanicus is the same species as T. kanchil,while T. napu have differences in body size compared to the previous species. The status of genus Tragulusin Indonesia are protected by the law and categorized as least concern for T. napu and data deficient for T.javanicus in the IUCN Redlist. The aims of this study was to look at genetic variation among species andkinship based on mitochondrial DNA gene genus Tragulus. Genes used in this study was 12S ribosomalRNA with 300 bp long, using eight samples orginated from Singkep Islands (four individuals), MountHalimun, West Java (three individuals) and East Kalimantan (one individual). As a comparisons, publishednucleotide sequence of each species from GeneBank database were used. The results showed that thegenetic distances (d) between individuals is high d = 0.322 ± 0.023 with the nucleotide diversity (ð) =0.2402 which indicates that samples was consisted of samples from different species. Phylogenetic treebased on the nucleotide analysis indicatedthat these samples composed of three species namely T. javanicus,T. kanchil and T. napu. Samples from Mt. Halimun and East Kalimantan are clustered in one group,namely T. kanchil (Borneo) and T. javanicus (Mt. Halimun). Two samples from Singkep are clustered indifferent groups with the possibility as T. napu.
Suplementasi Ranggah Muda Rusa Sambar Memperbaiki Pertumbuhan Tulang Femur, Bobot Otot, dan Ketahanan Fisik Tikus Putih (THE EFFECT OF SAMBAR VELVET ANTLER SUPLEMENT ON FEMUR BONE, BODY GROWTH, AND PHYSICAL ENDURANCE IN RAT) Gono Semiadi; Raden Taufiq Purna Nugraha; Yuliasri Jamal
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Antlers are deer’s bony organ that follows a cycle of growing, hardening, casting and regroupingwithin a certain period. The effect of consuming velvet antler from temperate origin has beenknown scientifically to have positive effect for rheumatism and metabolic disorder sickness therapy.However, the role of velvet antler originated from tropical deer has not yet been explored. Thisstudy aimed to assess the potential of the velvet antler of sambar deer (Rusa unicolor) which wasexperimentally fed to laboratory rats. The assessment was made based on the animals growth rate(i.e. femur length, weight of testicle, body eight) and physical endurance (i.e. swimming test).Laboratory rats at 21 days old were allocated into four different groups and each group consisted offive rats were fed with powder of soft and hard parts of velvet antler at dose of 0, 1, 2, and 3 g/kgbody weight, respectively. Animals were examined for eight weeks the body weight was examinedweekly and the dose at velvet antler supplement was adjusted accordingly. At the end of the studythe rat were put on endurance swimming test and then euthanized, for measurement of femurbone length and weight of testis. The results showed that there were no differences in the bodyweight. However at dose of 2 g soft part/kg BW indicating a consistently higher live weight gainsacross the observation time. Testis weight showed no significant differences between the treatments,but the length of femur bone showed a significant effect (p <0.05) with the doses level, with thehighest score being at 3 g hard part / kg BW. Physical endurance showed a significant effect (p<0.05) with the doses level, with the level of 1 g soft part/kg BW gave the best performance.
Co-Authors GONO SEMIADI Wirdateti . YULIASRI JAMAL