Sumpono Sumpono
University of Bengkulu

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UJI EFEKTIVITAS ASAP CAIR CANGKANG BUAH Hevea braziliensis TERHADAP AKTIVITAS BAKTERI Escherichia coli Dinda Oktarina; Sumpono Sumpono; Rina Elvia
ALOTROP Vol 1, No 1 (2017)
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/atp.v1i1.2704

Abstract

This research purpose to determine the effectiveness of the liquid smoke of the Hevea braziliensis  fruit shell  against the activity of Escherichia coli bacteria. The preparation and purification of liquid smoke is carried out by 4 stages: pyrolysis , sedimentation, stage 1 and stage  2  distillation. This research was started with total acidity test, phenol level, pH and density with result of measurement 4,725% total acid content, 0,84% phenol content, 2,548 pH and 1,004 in density . An antibacterial test  using disc diffusion method by looking at the diameter of the clear zone. There are six concentrations of liquid smoke of  H.braziliensis  rubber shell used, negative control using aquades and positive control using ciprofloxacin . An  effective concentration was found in inhibiting E.coli is 100% concentration with a clear zone diameter of 11.3 mm. The result of analysis using One Way ANOVA showed F count (4.42)> F table (3,23), then H0 rejected, meaning there is a significant difference of influence of concentration to the diameter of clear zone produced.
AKTIVITAS ASAP CAIR CANGKANG BUAH Hevea braziliensis SEBAGAI ANTI BAKTERI Staphylacoccus aureus Widiya Agustina; Sumpono Sumpono; Rina Elvia
ALOTROP Vol 1, No 1 (2017)
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/atp.v1i1.2705

Abstract

The aims of this research is to know the activity of liquid smoke Hevea braziliensis  Seed Shell as antibacterial of Staphylacoccus aureus. The synthesis of  liquid smoke on this research through some process such as pyrolysis, sedimentation, filtration, distillation and redistillation. H.braziliensis Seed Shell liquid smoke  obtained phenol levels 0.84% and pH  2.548. Antibacterial activity tested by hollow difusion methode with variations concentration of liquid smoke .at 5%, , 10 , 25 , 50, 75, and 100 % v/v. The effective concentration H.braziliensis  liquid smoke in inhibiting the growth of S. aureus bacteria is 75% v/v with strong antibacterial level that showed by an inhibition zone at 10,6 mm. On this research One Way ANOVA test showing the Fcount   ? F table, which means there is a significant difference between the diameter of the inhibition zone on various concentrations of H.braziliensis seed shell.iquid smoke..