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U. Paputungan
Faculty of Animal Sciences, Sam Ratulangi University, Jl. Kampus Unsrat Bahu, 95115, Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Veterinary

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THE ESTIMATION ACCURACY OF LIVE WEIGHT FROM METRIC BODY MEASUREMENTS IN ONGOLE GRADE COWS Paputungan, U.; Hakim, L.; Ciptadi, G.; Lapian, H.F.N.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 3 (2013): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.3.149-155

Abstract

length in North Sulawesi province. Data on animal live weight (LW), body length (BL) and chest girth(CG) were collected from all cows (n=363) kept by traditional household farmers. Regression analysiswas carried out to estimate LW from all linear body measurements. Data were classified based on age ofanimals consisted of five groups with the first age group of two and half years old, to the fifth age groupof seven and half years old. The results showed that age significantly (P<0.05) influenced all bodymeasurements. Correlations between all pairs of measurements were highly significant (P<0.001) for allage groups. Regression analysis showed that live weight could be predicted accurately from chest girthand body length (R2=0.97). Multiple regression model can be recommended to predict live weight ofOngole grade cows based on those variables with their age groups ranging from 2.5 to ≥7.5 years old asfollows: Live weight (kg) = -806.410 + 4.79835 CG (cm) + 2.83500 BL (cm).
DETECTION OF MENDELIAN AND GENOTYPE FREQUENCY OF GROWTH HORMONE GENE IN ONGOLE CROSSBRED CATTLE MATED BY THE ARTIFICIAL INSEMINATION TECHNIQUE Paputungan, U.; Hakim, L.; Ciptadi, G.; Lapian, H.F.N.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.79-85

Abstract

The objectives of this study were to detect the Mendelian mode inheritance of growth hormone(GH) and to establish genotype frequency of GH gene in Ongole-crossbred cattle mated by the artificialinsemination (AI) technique. Total of 76 blood samples were collected from Ongole-crossbred cows andbulls (G0), and their progenies (G1) at the Tumaratas AI service center in North Sulawesi province,Indonesia. All blood samples were screened for the presence of GH locus using a PCR-RFLP methodinvolving restricted enzyme Msp1 on 1.2 % of agarose gel. Data were analyzed using statistical programfunction in Excel XP. The results showed that GH locus using alleles of Msp1+ and Msp1- enzymerestriction in Ongole-crossbred cows and bulls was inherited to their Ongole-crossbred progeniesfollowing the Mendelian mode inheritance. This Mendelian inheritance generated by AI technique wasnot under genetic equilibrium for the Msp1 genotype frequencies in groups of G0 and G1. The breedingprogram using genotypes of bulls and cows (G0) for generating the genotype of GH Msp1 enzymerestriction by AI technique should be maintained to increase these various allele dispersion rates forbreeding under genetic equilibrium of the Ongole-crossbred cattle population.
HERITABILITIES OF BODY SIZE BY GROWTH HORMONE (GH-Msp1) GENOTYPES USING PCR-RFLP IN ONGOLE GRADE CATTLE Paputungan, U.; Hakim, L.; Ciptadi, G.; Lapian, H. F. N.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.138-144

Abstract

Genotypic performance in term of heritability as the crucial factor of animal economical traits for body size inheritance had not been fully studied in Ongole-grade cattle. The objectives of this research were to define the heritability values of live weight, chest girth and body length in Ongole-crossbred cattle. Total of 37 blood samples were collected from parental cows and 2 blood samples from parental Ongole breed bulls. All blood samples were screened for the presence of growth hormone (GH) locus using PCR-RFLP method involving restricted enzyme Msp1 on agarose-gel (1.2%). Data were analyzed using statistical program in Excel XP. Results showed that the phenotypic estimation average of Ongole grade cattle population of live weight, chest girth and body length were 445.41 ± 45.95 kg, 175.35 ± 4.11 cm, and 139.70 ± 5.73cm, respectively. The heritability values of animal live weight, chest girth and body length in this study were 0.24, 0.003, and 0.41, respectively. These heritability values of animal live weight and body length would be categorized as moderate to high genotypic performance values, while the heritability of animal chest girth was included in low heritability standard of the animal economical trait performance.
Comparison of the favorable gain values of genetic improvement among Indonesian grade cow breeds selected for agrotechnopark intensification Paputungan, U.; Hendrik, M. J.; Siswosubroto, S. E.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 2 (2021): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.2.106-113

Abstract

This research was aimed to compare the small and big truncation point proportions intended to evaluate gain values of genetic improvement for Indonesian Local cow breed groups selected for Agrotechnopark (integrated bio-cycle farming system) intensification. Animal live weights were collected from 674 Indonesian grade breed cows kept by local household farmers in North Sulawesi province. Data of cows were corrected by adjusting to six years old ages. All cows were divided into three breed groups with different genetic compositions of Bali breed cow generation (BG) of 207 cows, Ongole grade cow generation (OG) of 189 cows, and Local grade cow generation (LG) of 178 cows. The genetic improvements of BG, OG and LG were analyzed involving selection intensity (i), accuracy of selection (r), and standard deviation (SD) of breed group traits under selection. Results of this study showed that the critical components was genetic development of local grade breeds by choosing small proportion of 10% truncation point for Agrotechnopark intensification of selected elite cows groups among BG, OG and LG populations with the positive live weight gains of 58.6 kg, 23.15 kg, and 28.62 kg per generation, respectively compared with larger percentages of 20% and 30% proportions of truncation points.
Analisis faktor konsentrat pakan terhadap konsumsi asam-asam amino ayam ras petelur Utiah, W.; Paputungan, U.
ZOOTEC Vol 41, No 1 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.707 KB) | DOI: 10.35792/zot.41.1.2021.31536

Abstract

ANALYSIS OF FEED CONCENTRATE FACTOR ON LAYING HEN AMINO ACIDS CONSUMPTION. Laying hens require a number of elements in which nutrients such as protein containing the amino acids are balanced in quality. Recently, there are various types of commercial ration (concentrate) for laying hens produced by the company. Quality of protein is expressed either high or low composition of amino acids depending on the essential amino acids contained in these feeds. The purpose of this study was to determine the effect of different types of commercial concentrates on the consumption of amino acids in laying chicken. The research was conducted on laying chicken farms located in Matali Village, district of East Kotamobagu, Kotamobagu Municipality. Laying hens of Silver Strains as many as 240 heads at 78-week-old were used in this study. This study applied a completely randomized design (CRD) consisting of three treatments and eight replications at each treatment. The treatments were using commercial concentrations of the amino acids for commercial Concentrate A (RA), commercial concentrate B (RB) and commercial concentrate C (RC). The laboratory analysis showed that the majority amounts of essential amino acids (Arginine, Lysine, histidine, leucine, isoleucine, valine, threonine and phenylalanine) of 80% was higher in RC than RB and RA, indicating that the composition of both quality and quantity of amino acids in commercial concentrates C (RC) is sufficient and more balanced than commercial concentrates A (RA) and commercial concentrate B (RB). These complete amino acids in RC increased more feed consumption of laying hens compared with those in RA and RB.Key words: Laying hens, amino acids, commercial concentrate
Makroskopik semen segar kambing bangsa Peranakan Etawa (PE), Boer dan Saanen di Balai Inseminasi Buatan Lembang Mokoagow, F.; Pudjihastuti, E.; Hendrik, M.J; Paputungan, U.
ZOOTEC Vol 41, No 1 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.821 KB) | DOI: 10.35792/zot.41.1.2021.32462

Abstract

MACROSCOPIC TEST OF FRESH SEMEN IN GOAT BREEDS OF ETTAWAH, BOER AND SAANEN AT LEMBANG ARTIFICIAL INSEMINATION CENTER. Research aim to evaluate the macroscopic quality of fresh semen in goat breeds of Ettawah, Boer and Saanen at Lembang Artificial Insemination Center. Direct observation was done to obtain primer data. Secondary data were found as additional data supporting primer data. Variables included in observation were involving semen volume, ejaculates, color, consistence, pH and smell. Semen qualities of three breed groups collected on three sequent months were analyzed by t-test method. Results showed that semen qualities for ejaculates from three breed groups of Ettawah, Boer and Saanen collected on different months of September, October and November were not significantly different with the averages of 2.47 ml, 2.64 ml and 2.70 ml respectively. Semen volume from three breed groups of Ettawah, Boer and Saanen collected on those different months were also not significantly different with the averages of significantly different with the averages of 2,30 2,64 and 2,60 mL, respectively. The percentages of color of fresh semen from Ettawah, Boer and Saanen collected on those different months were indicating cream of semen collected on September, October in Ettawah Grade, Boer and Saanen. Fresh semen of these breeds was indicating yellow color collected on November, but it was showing normal condition. Fresh semen of these breeds collected on those months were totally indicating thick consistency. However, pH of fresh semen in all three breeds of goat collected on September and October were significantly different with those collected on November. The pH of fresh semen in these breeds collected on September and October were not significantly different. Values of pH in these breeds collected on September, October and November were 6.63, 6.65 and 6.70, respectively. These values were ranging in normal condition. The smell of fresh semen in these breeds collected on those series of months was indicating specific rancid smell.Key words: Fresh semen quality, goat breed, semen collection period
Pengetahuan peternak tentang pemahaman keterkaitan gejala birahi dengan keberhasilan inseminasi buatan pada sapi di Kecamatan Pinolosian Dawit, G.; Paputungan, U.; Podung, A.
ZOOTEC Vol 41, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.213 KB) | DOI: 10.35792/zot.41.2.2021.36884

Abstract

Salah satu aspek pemicu rendahnya populasi ternak sapi adalah manajemen reproduksi yang kurang tepat seperti manajemen perkawinan hingga pengetahuan peternak tentang teknologi inseminasi buatan. Penelitian ini bertujuan untuk mengetahui sejauh mana peternak sapi di Kecamatan Pinolosian Kabupaten Bolaang Mongondow Selatan Sulawesi Utara memahami keterkaitan gejala birahi dengan inseminasi buatan (IB) pada sapi. Responden penelitian ini adalah peternak sapi di Kecamatan Pinolosian dan bersedia diwawancarai. Penelitian ini dilakukan dalam bentuk survei lapangan. Pengumpulan data dilakukan dengan mewawancarai peternak sapi menggunakan formulir dengan kuisioner terstruktur. Pengambilan data terdiri atas data primer dan sekunder. Data sekunder di ambil dari Dinas Pertanian dan Ketahanan Pangan Kabupaten Bolaang Mongondow Selatan. Data dianalisis menggunakan metode deskriptif untuk keseluruhan data, uji linear berganda untuk variabel pendidikan, pekerjaan, kedudukan beternak, jumlah ternak, pengalaman beternak, sumber pengetahuan dan cara pemeliharaan dengan variabel pengetahuan tentang kaitan gejala birahi dengan keberhasilan IB. Berdasarkan hasil penelitian tingkat pengetahuan peternak tentang pemahaman keterkaitan gejala birahi dengan keberhasilan IB pada sapi dengan kriteria sangat baik adalah 0%, peternak baik 9,23%, peternak cukup baik 66,15% dan peternak kurang baik 24,61%. Sebesar 43,8% pengetahuan tentang birahi dan keberhasilan IB dipengaruhi oleh faktor pendidikan, pekerjaan, kedudukan beternak, jumlah ternak, pengalaman beternak, sumber pengetahuan dan cara pemeliharaan ternak.Kata kunci: Tingkat pengetahuan peternak, inseminasi buatan, gelaja birahi, sapi lokal
PENGELOLAAN LIMBAH KOTORAN TERNAK DALAM UPAYA PENCEGAHAN PENCEMARAN LINGKUNGAN DI KOTA KOTAMOBAGU Linggotu, Lidyasanty O.; Paputungan, U.; Polii, B.
ZOOTEC Vol 36, No 1 (2016)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.112 KB) | DOI: 10.35792/zot.36.1.2016.10535

Abstract

ANIMAL WASTE PRODUCT MANAGEMENT FOR PREVENTION OF ENVIRONMENTAL POLLUTION IN KOTAMOBAGU CITY. The animal wastes from animal farm affect the environmental condition of human settlement, when it was not well managed. The objectives of this study was to evaluate either levels of knowledge, attitude and technical skill had correlated directly or not with their motivation activity in preventing environmental pollution of animal wastes around Kotamobagu city. Variables in this study were referring on the farmer’s standard knowledge (X1), attitude (X2), technical skill (X3) and motivation activity in preventing environmental pollution of animal wastes (Y) around Kotamobagu city using the functions of linear multiple regression, Yi = b0 + b1X1 + b2X2 + b3X3 + ei; where Yi was the-i observation of farmer’s  motivation activity in preventing environmental pollution of animal wastes; b0 was intercept; b1, b2 and b3 were regression coefficients, X1 was farmer’s knowledge, X2 farmer’s attitude, X3 was farmer’s technical skill in preventing environmental pollution of animal wastes; and e1 was random error. Results of this study showed that knowledge level (X1), attitude (X2) and technical skill (X3) of farmers could be the important factors affecting farmer’s motivation activity in preventing environmental pollution of animal wastes (Y) in Kotamobagu city areas.  The equation of multiple regression model was found, Y = 1.468 + 0.784 X1 + 0.218 X2 + 0.004 X3. The determination coefficient (R2) of 0.81 in this model indicated that 81 percents of the variation of independent variables (X1, X2, and X3) used in the model were able to affect the variation of the dependent variable (Y). The rest of 19 percents in the model were caused by the other unknown factors. The average score values of knowledge level (X1=87.23), attitude (X2=93.37) and technical skill (X3=88.40) and farmer’s motivation activity (Y=90.60) in preventing environmental pollution of animal wastes (poultry and pig) in Kotamobagu city areas were still below the score median values (X1=93; X2=100; X3=100; Y=93) of those variables. This condition suggested that extension activities related to the prevention of environmental pollution of animal wastes in Kotamobagu city areas were still needed to be conducted, mainly to the farmers with low scores in knowledge, attitude, technical skill and motivation activity in preventing environmental pollution of animal wastes. Key words: Animal waste product, prevention of environmental pollution, Kotamobagu city.
Co-Authors B. Polii, B. Dawit, G. E. Pudjihastuti G. Ciptadi H. F. N. Lapian, H. F. N. H. F.N. Lapian H.F.N. Lapian Hendrik, M. J. Hendrik, M.J J. Kasehung, J. J. Paath, J. L. Hakim Lidyasanty O. Linggotu, Lidyasanty O. Manopo J. H. Mokoagow, F. Podung, A. S. Adiani Siswosubroto, S. E. Trisya T. Makalalag W. Utiah