Garuda - Garba Rujukan Digital

The isolation of Gurnbiro virus from larvae and darkling Ivelles (Carcinops pumilin) Parede, Lies; Indriani, Risa; ., Sukarsih
Indonesian Journal of Animal and Veterinary Sciences Vol 2, No 1 (1996)
Publisher : Indonesian Animal Sciences Society

Gumboro (infectious bursal disease, IBD) virus was isolated from darkling beetles (Carrinaps pumilin) and their larvae in a commercial pulletchicken farm with repeated outbreaks incidence of Gumboro disease in Tangertng, West Java. In addition, these over populated beetles and their larvae were suspected to be infected and then shed the virus or acted as vectors. Isolation was done by repeated passages of virus using chicken embryo fibroblast cells as prime media, which then showed the evidence of cylop: ihic effecis. The isolation was followed by antigen detection by means of ELISA test. Â Key words: Gumboro disease, infectious bursal disease, darkling beetle, Carcinops punulin Â

The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Wahyuwardani, Sutiastuti; Huminto, Hernomoadi; Parede, Lies
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 1 (2005)
Publisher : Indonesian Animal Sciences Society

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with Â± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation. Â Â Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption

The macroscopic and microscopic patology changes on broiler infected with local reovirus isolate Sutiastuti Wahyuwardani; Hernomoadi Huminto; Lies Parede
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Local reovirus isolate is the virus which could be isolated from runting and stunting syndrome. The ability of local reovirus isolate to induce runting and stunting syndrome in broilers chicken was investigated. The day old chicks (doc) were infected with local reovirus isolate assessed clinically and pathologically at 1, 2 and 3 weeks post inoculation. A total of 40 DOC were divided into two groups. The first group (20 doc) was orally infected with ± 2 x 103 local reovirus isolate particle and the other used as negative control group. The results showed that the isolate caused wet droppings, stunting, enteritis, pancreatitis, malabsorbtion, bursal atrophy and spleenic hypertrophy, which similar to runting and stunting syndrome (RSS) disease in chicken. The body weight was reduced to 14.7% on the inoculated group at 4 weeks post inoculation. Key Words: Broiler, Local Reovirus Isolate, Runting and Stunting, Malabsorption

The isolation of Gurnbiro virus from larvae and darkling Ivelles (Carcinops pumilin) Lies Parede; Risa Indriani; Sukarsih .
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 1 (1996)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Gumboro (infectious bursal disease, IBD) virus was isolated from darkling beetles (Carrinaps pumilin) and their larvae in a commercial pulletchicken farm with repeated outbreaks incidence of Gumboro disease in Tangertng, West Java. In addition, these over populated beetles and their larvae were suspected to be infected and then shed the virus or acted as vectors. Isolation was done by repeated passages of virus using chicken embryo fibroblast cells as prime media, which then showed the evidence of cylop: ihic effecis. The isolation was followed by antigen detection by means of ELISA test. Key words: Gumboro disease, infectious bursal disease, darkling beetle, Carcinops punulin

PHYLOGENETIC ANALYSIS OF NUCLEOTIDE SEQUENCE OF HIPERVARIABLE FRAGMENT OF VP2 OF INFECTIOUS BURSAL DISEASE VIRUS ISOLATED IN INDONESIA I Gusti Ngurah Kade Mahardika; Lies Parede
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.098 KB)

Nucleotide sequence of hypervariable fragment of VP2 of infectious bursal disease virus (IBDV) isolated in Bali and Indonesia were compared to standard virulent (svIBDV’s) and very virulent (vvIBDV’s) viruses accessed in GeneBank. The comparation were done using the multiple sequence alignment program of Mega 3.1. The sequence were aligned by the ClustalW Method (Mega 3.1), that is grouping the sequences into clusters by examining the distance between all pairs. The sequences were aligned pair wise, then as groups. Phylogenetic tree were constructed using Neighbor-Joining and Bootstrap-tested. The analysis showed that IBDV can be clustered into 2 major groups, i.e. America-Europe and Australia clusters. The America-Europe cluster is further divided into two sub-clusters, i.e. classic IBD and vv-IBD. Classic-IBD is represented by standard STC, Cu-1, Variant A, F52-70, and PBG98 viruses. Two IBDV’s isolated in Bali and the most of other Indonesian isolates belong to sub-cluster vv-IBD and share a common cluster with IBDV that were recently reported as very virulent strains. One exceptional isolate is Indo 13, which is groupped into classic IBD, and closely related to classical American standard virus, STC.

Patogenesitas Virus Gumboro Isolat Lokal pada Ayam Pedaging Sutiastuti Wahyuwardani; Dewi Ratih Agungpriyono; Lies Parede; Wasmen Manalu
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.26 KB)

Study of the pathogenicity of gumboro virus (very virulent Infectious Bursal Disease virus, vvIBDV) oflocal isolates was performed in broilers. The chickens were grouped into four: i) infected with vvIBDV; ii)vaccinated with commercial vaccine + challenge with vvIBDV; iii) vaccinated using locally produced vaccine+ challenged with vvIBDV; and iv) control group (unvaccinated animals). Pathogenecity was analyzedbased on the sequence of infection; the distribution and degree of gross pathology and histopathologylesions on bursa Fabricius, spleen and thymus in relation to the presence of the antigen (IBDV Ag) whichwas detected using immunohistochemistry technique. During the acute phase (1-3 days following theexperimental infection) hyperemia and exudation on the bursa Fabricius were observed in the infectedgroup and the two vaccinated-challenged groups. Similarly, hyperemia was also observed on the spleenand thymus. Whilst during the chronic stages (at 7 and 14 days following the experimental infection) thebursa showed atrophy. Upon histological examination, the bursa showed interstitial edema, hemorrhage,infiltration of heterophyl cells and fibroblast hyperplasia. The bursa lymphoid follicles were depleted dueto cells necrosis and apoptosis. Plica epithelial layer was wrinkled and the Goblet cells metaplasia formedhyper plastic cysts. In the spleen and thymus severe reticulo endothelial cells proliferation was observedduring the acute stage and tended to decline during the chronic stage. During the acute and chronic stagescells containing IBDV Ag were detected in the bursa Fabricius, distributed within the mantle zone andgerminal center of lymphoid follicles in the two vaccinated-challenge groups. Whereas in the infectedgroup, the IBDV antigen were detected up to 7 days post the infection. Nevertheless, neither IBDV antigenwas detected in the spleen nor in the thymus in all the experimental groups. The results of this studyclearly explained the ferocity of vvIBDV of local isolates.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search