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ACETONE-BUTANOL-ETHANOL FERMENTATION FOR BIOENERGY USING VARIOUS SUBSTRATES IN DEFINED TYA MEDIA Hanies Ambarsari; Kenji Sonomoto
Jurnal Energi dan Lingkungan (Enerlink) Vol. 11 No. 1 (2015)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/elk.v11i1.1590

Abstract

A series of experiments had been conducted to investigate the performance of Clostridiumsaccharoperbutylacetonicum strain N1-4 (ATCC 13564) in direct fermentation of acetone, butanol, andethanol (ABE) using several substrates that have not been investigated previously using this strain,such as cellobiose, dextrin, starches of corn, potato, tapioca, wheat, and sago, as well as morecomplex carbohydrates such as xylans, cellulose, and carboxyl methyl cellulose (CMC), particularly tostudy the effect of such various substrate types and concentrations, as well as the substrates mixtureson the ABE ratio and productivity. The results showed that strain N1-4 could directly produce ABE fromvarious substrates, from monosaccharide to polysaccharides of hexose or pentose sugars, exceptCMC and cellulose. The experiments also suggested that cellobiose (a hexose disaccharide) could bethe more efficient substrate for strain N1-4 than glucose (a monosaccharide) to produce ABE in the TYA(Tryptone Yeast Agar) medium. It was proven also that the ABE ratio was very dependent on the typeand concentration of substrates being used by strain N1-4.Keywords: acetone, butanol, ethanol, clostridium saccharoperbutylacetonicum N1-4, fermentation
Biobutanol Production Using Fed-Batch High-Cell Density Extractive Fermentation Rizki Fitria Darmayanti; Yukihiro Tashiro; Kenji Sakai; Kenji Sonomoto
Journal of Biobased Chemicals Vol. 1 No. 1 (2020): Journal of Biobased Chemicals
Publisher : Program Studi Teknik Kimia Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (782.366 KB) | DOI: 10.19184/jobc.v1i1.108

Abstract

Butanol, as a product with specific toxicity for its producer, is necessary to be maintained in low concentration during fermentation. In-situ integrated recovery using extensive volume extraction was used in high cell density fermentation in order to prevent the lag phase in the prime condition. Clostridium saccharoperbutylacetonicum N1-4 ATCC 13564 was employed to ferment glucose in extractive fermentation with oleyl alcohol as extractant. As the results of fed-batch cultures using high cell density with different extractant to broth volume ratios (Ve/Vb), 0.8 g/l butanol concentration in the broth was maintained with a ratio of 10, which was much lower than 4.4 g/l with the ratio of 0.5. Besides, the Ve/Vb ratio of 10 demonstrated 2.7-fold higher total butanol concentration (28 g/l) than that 11 g/l obtained with a Ve/Vb ratio of 0.5. These results indicated that larger Ve/Vb improved total butanol concentration by reducing butanol toxicity in broth.