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All Journal Berkala Penelitian Hayati
Y. Sriwulan M.
FMIPA Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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ANALISIS 6 DNA REKOMBINAN DENGAN ENZIM EcoR1 Ni Nyoman Tri Puspaningsih; Akhmaloka; Afaf Baktir; Ami Soewandi J.S.; Y. Sriwulan M.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 3 No 1 (1997): June 1997
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/490

Abstract

Amylase enzyme from Endomycopsis fibuligera capable to hidrolize starch into glucose. Insertion of amylase gene from Endomycopsis fibuligera into yeast (Saccharomyces cereviceae) will be able to increase the function of yeast (S.cereviceae) to digest more cheaper substrate, like starch. Before cloning in yeast, recombinant DNA will be made and analyzed in Escherichia coli strain DH5a. The result showed that the sixth transformant consist recombinant DNA that were sensitive to tetracycline medium. Analysis by EcoR1 digestion showed that the size of insertion fragment into Ycp 50 vector are around 0.3 untill 16 kb.
Co-Authors AFAF BAKTIR Akhmaloka Akhmaloka Ami Soewandi J.S. Ni Nyoman Tri Puspaningsih