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Novi Sulistyaningrum
Pusat Biomedis dan Teknologi Dasar Kesehatan, Badan Litbangkes, Kemenkes RI
Medicine & Pharmacology

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Uji Mutagenik Ames untuk Melengkapi Data Keamanan Ekstrak Gambir (Uncaria gambir Roxb.) Novi Sulistyaningrum; Sukmayati Alegantina; Lina Rustanti
Jurnal Kefarmasian Indonesia VOLUME 3, NOMOR 1, FEBRUARI 2013
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

The main compound of Uncaria gambir Roxb. (gambir), catechin and it’s derivates have been believed to be potential as antiviral. Epigallocatechin gallate (EGCG) and epicatechin are catechin derivates which are found to be potential as antiviral against Human Immunodeficiency Virus (HIV). However, gambir extract also contains quercetin that has possibility to be mutagenic. Therefore, a preliminary study towards safety of those compounds within gambir extract, mutagenicity assay using Ames Method has been performed.Sample (gambir extract) was obtained from West Sumatera, Indonesia. The extract was characterized according to Farmakope Herbal Indonesia and WHO methods. Mutagenicity test by Ames method utilized a colorimetric microplate in 6 various concentration (125 mg/mL; 62.5 mg/mL; 31.25 mg/mL; 15.625 mg/mL; 7.81 mg/mL dan 3.91 mg/mL) against mutant bacteria Salmonella typhimurium TA 98, Salmonella typhimurium TA 100 and Escherichia coli WP2 uvrA with and without the addition of S-9 enzyme. Extract of gambir in this study contains 86.60% of catechin, 12.92% moisture content, 22.49% water-soluble extract content, 80.63% ethanol-soluble extract content, 0.81% total ash, 0.32% acid insoluble ash content and 10.38% in dryness level. From the mutagenicity test and calculation, fold increase (over baseline) of the sample in 6 various concentration with and without adding S-9 enzyme are lower than 2. Gambir extract from West Sumatra with catechin contains 86.6% hasn’t showed mutagenic effect due to the fold increase (over baseline) of mutagenicity test lower than 2.
Isolasi dan Identifikasi Struktur Karotenoid dari Ekstrak Bayam Merah (Amaranthus Tricolor L.) Novi Sulistyaningrum
Jurnal Kefarmasian Indonesia VOLUME 4, NOMOR 2, AGUSTUS 2014
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Amaranthus tricolor L. was reported as source of carotenoid compounds (tetraterpenoids) which is a natural pigments and has bioactivity as antioxidants. They are beneficial to health. Carotenoids isolate are obtained by maceration method in methanol, saponification with KOH / MeOH 5%, fractionated by vacuum column chromatography, preparative thin layer chromatography (preparative-TLC) repeatedly and each fraction monitored using thin layer chromatography (TLC). Structure analysis of carotenoids based on UV, IR and GCMS spectroscopic data showed similarities as lutein structure.
Co-Authors Lina Rustanti Sukmayati Alegantina