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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science Majalah Obat Tradisional JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Aquaculture and Fish Health Jurnal Perikanan Pantura Jurnal Pengabdian kepada Masyarakat (Ngabdimas) Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
R Adharyan Islamy
Departement Of Aquaculture, Faculty Of Fisheries And Marine Science, Brawijaya University Malang
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Journal : Research Journal of Life Science

 1 
Potential Analysis of Lemna sp. Extract as Immunostimulant to Increase Non-Specific Immune Response of Tilapia (Oreochromis niloticus) against Aeromonas hydrophila Eric Armando; Ayu Lestiyani; R. Adharyan Islamy
Research Journal of Life Science Vol 8, No 1 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.01.6

Abstract

Lemna sp. is known to have several bioactive compounds and polysaccharide macromolecules that can function as immunomodulators to affect non-specific immune responses to increase the body's resistance to pathogens. This study aims to determine the potential of catfish eye extract as an immunostimulant by observing non-specific tilapia immune parameters. The extraction method used was 96% ethanol maceration for 2 days with a ratio of 1: 4. The experimental design used a Completely Randomized Design with 5 treatments (doses 0.2, 0.4, 0.6 mg/kg, control + and control -) and 3 replications. The non-specific parameters of immunity observed included total plasma protein (Bradford method), superoxide dismutase and lysozyme activity. The data obtained will be analyzed using ANOVA, if there is a significant difference, it will be further tested with Duncan Multiple. Range Test (DMRT). The results showed that the highest total plasma protein was found in treatment C (giving an extract of 0.3 mg/kg body weight) with an average total plasma protein after 12 days of maintenance of 4.99 g / dL. The extract dose of 0.3 mg/body weight showed a rapid decrease in SOD and increase Lysozyme activity.
Phytogenetic Relationships of Mystacoleucus marginatus (Valenciennes 1842) based on Cytochrome Oxidase C Subunit I (COI) Gene Fitri Sil Valen; Maheno Sri Widodo; Yuni Kilawati; R. Adharyan Islamy
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.3

Abstract

Mystacoleucus marginatus is a type of freshwater fish that is spread in various countries with tropical climates, one of which is Indonesia. This fish can be found in the Brantas River Basin (DAS), East Java. In its development, identification of fish species needs to be done in detail and more deeply, so that the correct identification results can be obtained. This study aims to identify genetic and phylogenetic characteristics of Mystacoleucus marginatus in the Brantas River Basin based on the Cytochrome Oxidase C Subunit I (COI) gene. Test samples were taken from 11 individuals of Mystacoleucus marginatus in the Brantas watershed, from June to August 2018. The results showed that Mystacoleucus marginatus in the Brantas watershed had 2 Haplotypes with Haplotype (Hd)=0.96 diversity values and nucleotides diversity (Pi)=0.00357. These results indicate that the population in the Brantas watershed is interconnected between one location and another sampling location, this is supported by a phylogenetic topology of DNA barcodes showing a monophyletic clade, as well as a haplotype distribution indicating that the sample area is genetics are not isolated from one another. So that the type of Mystacoleucus marginatus scattered along the Brantas watershed is still in one species, which is indicated by the low level of genetic variation. Genetic identification efforts must be carried out continuously so that appropriate information can be obtained for the diversity of fish species, especially endemic fish.
Co-Authors Abdul Rahem Faqih Agoes Soeprijanto, Agoes Artanti Tri Lestari Asus Maizar Suryanto H Aulia Nadhirah Ayu Lestiyani Ayu Winna Ramadhani Diana Aisyah Edi Purwanto Eric Armando Fadli Mulyadi Faisal Hakim Fitri Sil Valen Indah Soraya Inggrida, Jedda Ayu Mahendri Rohman Ghanim Maheno Sri Widodo, Maheno Sri MOHAMAD FADJAR Muhammad Safir Novalina Serdiati Octovianus Petrus Senas Septian Maulana Purnama Umul Rezkiyah Uun Yanuhar Veryl Hasan Wahyu Isroni Yuni Kilawati Yunita Maimunah