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Ikhsan Khasani

Research Institute for Freshwater Fish Breeding and Aquaculture, Sukamandi

Author-ID : 3672349

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles

RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii Imron Imron; Dadan Sunandar; Rini Susilowati; Rommy Suprapto; Ikhsan Khasani
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Indonesia is rich of giant fresh water prawn (GFP) germ plasms. Best utilization of these resources for the purpose of either aquaculture development or conservation of genetic resources requires some information on the structure and levels of their genetic diversity. This study was aimed to characterize those GFP genetic resources by applying RAPD genetic markers. Six Indonesian populations of GFP from Asahan, Barito, Ciasem, Ogan, GImacro and Papua were collected and analyzed for their genetic variation using five RAPD primers. The results showed the diversity within the populations, as revealed by the level of polymorphism, ranged from 29% to 76% while genetic divergence between populations as shown by genetic distance ranged from 0.04 to 0.50. In terms of genetic divergence, two genetically distinct groups of GFP, namely the Papua GFP in one group and the remaining five GFP populations in the other, were identified. The results also showed the presence of specific population markers that are useful for genetic identification of GFP populations. Implication of these finding with regard to breed development is discussed.

PRELIMINARY STUDY OF FISH CULTURE IN ABANDONED SAND MINING POOL Bambang Gunadi; Lukas Dharma; Ikhsan Khasani; Yosmaniar Yosmaniar; Lies Setijaningsih
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

One of main problems in freshwater aquaculture development in Indonesia, especially in Java, is unavailability of developing zone. It is important to find an underutilized area that meets for industrial scale freshwater aquaculture, i.e. sufficient water supply, wide area, and located in one area or zone. The abandoned mining (sand, tin, etc.) pools distributed along the country might be the potential area for freshwater aquaculture business. For example, there are at least 13 water pools with total surface area of 250 ha at 15 km side of Citarum River in Karawang District (West Java Province). This study was conducted to obtain preliminary data about the prospect and potency of fish culture (tilapia, clariid catfish, and ‘patin’ catfish) in abandoned sand-mining pools in Karawang District. Mini floating net cages of 1 x 1 x 1.5 m3 size were used for culturing fish, i.e. patin catfish (Pangasianodon hypophthalmus), nile tilapia (Oreochromis niloticus), and clariid catfish (Clarias gariepinus), separately. Patin catfish were stocked at a size of 2 g with a density of 300 fish per cage, tilapia were stocked at a size of 6 g with a density of 400 fish per cage, while the clariid catfish were stocked at a size of 1.4 g with a density of 980 fish per cage. A floating commercial feed (30%—32% protein, 3%—5% fat) was used at a daily rate of 9% biomass weight at the beginning and reduced gradually to 3% at the final culture period. Observed data showed that patin catfish grew from the initial size of 2.08 g to the final size 299.59 g in 5 months, nile tilapia grew from individual initial size of 5.92 g to the final size of 247.12 g in 14 weeks, and clariid catfish grew from initial size of 1.39 g to the final size of 73.10 g in 8 weeks. These three species were technically prospective for aquaculture development in the abandoned sand-mining pools.

PRELIMINARY STUDY ON THE EFFECT OF VITAMIN C ON THE GROWTH AND SURVIVAL RATE OF THE FRESHWATER PRAWN (Macrobrachium rosenbergii) LARVAE Ikhsan Khasani
Indonesian Aquaculture Journal Vol 3, No 1 (2008): (June 2008)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

This experiment was conducted to investigate the influence of vitamin C on growth and survival rate of freshwater prawn larvae. The treatment dosages of vitamin C were 0.0, and 0.75 mg/L. Each treatment was in triplicates and arranged in a complete randomized design. Vitamin C was given to the larval rearing media every three days. Larvae were cultured in six conical fibreglass tanks of 50 L volume installed in a fibreglass tank (2 m x 0.8 m x 0.8 m) filled with water for temperature stabilizer. Each conical tank was stocked with 6,500 larvae (nauplii). Artemia nauplii were given on D3 and egg custard as artificial feed on D9. Larval rearing was done for 25 days in constant temperature (29oC–31oC) using automatic heater. Statistical analysis showed that metamorphosis speed of larvae was significantly affected by adding of vitamin C (P<0.05), but survival rate was similar (P>0.05). Addition of vitamin C also resulted in a better growth of freshwater prawn larvae.

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