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Ketut Mahardika

Research and Development Institute for Mariculture, Gondol, Bali

Author-ID : 3672354

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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HISTOLOGICAL STUDY ON INTERNAL ORGANS DEVELOPMENT OF MANGROVE SNAPPER, Lutjanus argentimaculatus LARVAE Ketut Mahardika; Indah Mastuti; Regina Melianawati; Zafran Zafran
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Mangrove snapper, Lutjanus argentimaculatus is one of highly economic value of marine fish commodity. Culture of this species has been developed a few years ago. Basic data on biological aspect is still limited whereas those kinds of information are needed. Histological study to determine development of internal organs of the larvae of mangrove snapper was conducted. Amount of 15-20 larvae each from 1 day after DAH hatching (1 DAH) up to 30 days after hatching (30 DAH) were collected, fixed in Bouin’s solution for 4-6 hours and then transferred into 70% alcohol. The samples were then embedded in paraffin wax and the sections were stained with haematoxylineosin (H&E). Results showed that the eye retina of the mangrove snapper larvae well developed on 3 DAH, whereas their digestive tract was on 5 DAH. Under this condition, the larvae started feeding as showed by present of natural food tissues in the intestine. Development of digestive tract and other internal organs reached their complete form like in adult fish after 20 DAH.

DISTRIBUTION ANALYSIS OF ENLARGED CELLS DERIVED FROM GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) INFECTED HUMPBACK GROUPER Cromileptes altivelis Indah Mastuti; Ketut Mahardika
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Characteristic of Megalocytivirus infection has been known to produce formation of inclusion body bearing cells (IBCs) on internals organs of fish predominantly on spleen and kidney. Megalocytivirus that infected grouper is known as Grouper Sleepy Disease Iridovirus (GSDIV). This study was conducted to answer the effect of entry sites of GSDIV on distribution of enlarged cells formed on the internal organs of humpback grouper Cromileptes altivelis. Enlarged cells were observed histologically under the light microscope on spleen, head kidney, trunk kidney, liver, gill, heart, stomach, intestine, muscle and brain. Entry sites were designated to intramuscularly injection, intraperitoneally injection, dipped gill and inoculum added feed. Enlarged cells were formed on spleen, head kidney, trunk kidney, liver, gill, heart, stomach, muscle, except on intestine and brain. All the entry sites resulted in formation of enlarged cells on spleen, head kidney, trunk kidney, liver, heart. Spleen and head kidney were the most frequent observed organ. These results suggested that distribution of enlarged cells were not affected by the entry site of GSDIV.

SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV) Ketut Mahardika; Indah Mastuti
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

RSIV is an isolate virus in the genus Megalocytivirus (family Iridoviridae) that has been reported to be pathogen in more than 31 marine fish species in East Asia. The aim of study was to know the susceptibility of several cultured cells to RSIV. RSIV inoculum was inoculated onto cultured cells and then incubated in 25oC. Routine observation of cytopatic effect (CPE) was carried out for 7 days and harvested cells were prepared for virus titration and electron microscopy (EM). The result showed that RSIV grew and propagated in GF (grunt fin), KF-1 (koi fin) and BF-2 (barfin flounder) which caused cytophatic effect as cel ls enlargement. However, RSIV did not propagated on EPC (epithelioma papulosum cyprini), FHM (feathed minnow) and EK-1 (eel kidney) cells. The virus titer were 105.3 TCID50/mL in GF cells, 103.8 and 4.3 TCID50/mL in KF-1, 103.6 and 3.8 TCID50/mL in BF-2, and 7 102.1 TCID50/mL in EPC, FHM and EK-1. The EM observation revealed formation of enlarged cells containing hexagonal virus particles with 140-160 nm in diameter. These results indicated that GF was cultured cell to be optimal for replication of isolate RSIV derived from Ise bay, Mie, Japan.

THE DIGESTIVE TRACT DEVELOPMENT OF THE TIGER GROUPER Epinephelus fuscoguttatus LARVAE TREATED WITH THYROXINE HORMONE Ahmad Muzaki; Ketut Mahardika; Indah Mastuti; Wawan Andriyanto; Yasmina Nirmala Asih; Ida Komang Wardana
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Thyroxin administration in tiger grouper larval rearing was conducted to determine its effect on development of digestive tract of the larvae. Newly hatched larvae were treated with 0 mg/L (control), 0.1 mg/L, and 0.2 mg/L of thyroxin for 2 hours in a 30 L tank before were transferred into a 1 m3 rearing tank. Samples were collected daily until 10 DAH and every 3 days until 40 DAH. Development of digestive tract was observed using histological method. For all treatments, the digestive tract was the same on 1 DAH and 2 DAH. The digestive tract of 1 DAH larvae was still closed and still in the form of a simple tube. The mouth, buccopharynx, liver, pancreas, and rectum were found on 2 DAH. On 3 DAH, the esophagus, stomach, intestines, and anus started to develop. At this stage, the digestive tract of larvae treated with and without thyroxin started to become distinguishable. Several goblet cells were noticed in the rectum of the treated larvae but not in the control. Digestive tract developed earlier in treated larvae compared to the control group, such as appearance of tongue, gill raker, fundic, and cardiac stomach development, formation of pharyngeal tooth, and gastric gland. Metamorphosis of larvae into juvenile on treated larvae occurred than that the control larvae earlier.

Co-Authors Ahmad Muzaki Ida Komang Wardana Indah Mastuti Regina Melianawati Wawan Andriyanto Yasmina Nirmala Asih Zafran Zafran

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