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Rommy Suprapto
Research Institute for Fish Breeding, Jl. Raya 2 Sukamandi, Subang 41256, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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EXPRESSION OF GROWTH HORMONE (PhGH) GENE AND ANALYSIS OF INSULINE-LIKE GROWTH FACTOR I (IGF-I) PRODUCTION IN AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC F-1 Huria Marnis; Bambang Iswanto; Rommy Suprapto; Imron Imron
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.581 KB) | DOI: 10.15578/iaj.8.2.2013.113-119

Abstract

We have previously produced F-1 transgenic of African catfish from crosses between founder transgenic female and non transgenic male. The aim of this study was to evaluate distribution and expression PhGH growth hormone gene transgenic African catfish organs and to measure the concentration of IGF-I in plasma. Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, gill, and eye. Transgene expression levels were analyzed using the method of quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR). Plasma samples were analyzed for Insuline-like Growth Factor (IGF-I) using Enzyme Linked Immunosorbent Assay (ELISA) method. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish (F-1). Liver exhibited the highest level of PhGH mRNA (23 x 106 copies). The plasma IGF-I levels in transgenic individuals were not significant than non transgenic. The higher level of exogenous PhGH gene expression may not represent the production of IGF-1.
HERITABILITY FOR GROWTH RELATED TRAITS IN GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) AT VARIOUS DEVELOPMENTAL STAGES AND CULTURE CONDITIONS ESTIMATED BY INTRACLASS CORRELATION Imron Imron; Rommy Suprapto
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1255.186 KB) | DOI: 10.15578/iaj.8.1.2013.35-45

Abstract

Heritability estimates of commercially important traits are of important in order to seek the best strategy of selective breeding program to be implemented. A study aimed at estimating the magnitude of this parameter for growth related traits, expressed in wet weight (WW), total length (TL), and standard length (SL), has been carried out in giant freshwater prawn (GFP). Particular emphasis was given to investigate the effect of ages and culture conditions on the magnitude of the heritability estimates. Nineteen full-sib families were established through individual pair mating. The families, namely groups of offsprings derived from each mating pair were raised through three stages of rearing activities: first-stage nursery (40 days), second-stage nursery (70 days), and grow-out rearing (130 days). Heritability for growth at each stage was estimated through the method of full-sib analysis or intraclass correlation. Components of variance used to produce the heritability estimates were obtained through the method of analysis of variance. Results showed that heritability estimates varied with both ages and culture conditions. The heritability estimates (± standard errors) at 40 days for WW (0.69±0.151), TL (0.64±0.148), and SL (0.70±0.144) were higher than those observed at 70 days (WW = 0.24±0.15; TL = 0.22±0.15; and SL = 0.20±0.14) and 130 days (WW = 0.24±0.058; TL = 0.22±0.05; and SL = 0.20±0.60). A similar pattern was found with respect to the culture conditions. The estimates found in grow-out at lower stocking density (5 individual/ m2) days (WW = 0.24±0.058; TL = 0.22±0.05; and SL = 0.20±0.60) were higher than those observed at grow out at higher stocking density (20 individuals/m2) days (WW = 0.12±0.058; TL = 007±0.05; and SL = 0.14 ± 0.60). The possible causes of the observed patterns and implications that these findings may have on the breeding program of GFP are discussed.
Co-Authors Bambang Iswanto Huria Marnis Imron Imron