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All Journal Indonesian Aquaculture Journal
Sukenda Sukenda
Research and Development Institute for Coastal Aquaculture, Jl. Makmur Dg. Sitakka No. 129 Maros 90512, South Sulawesi, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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SPECIFICITY OF Haemolysin AND Gyrase GENE MARKER FOR RAPID DETECTION OF VIBRIOSIS ON PENAEID SHRIMP Ince Ayu Khairana Kadriah; Sukenda Sukenda; Munti Yuhana; Endang Susianingsih; Muharijadi Atmomarsono
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (918.882 KB) | DOI: 10.15578/iaj.8.1.2013.47-54

Abstract

Pathogenic Vibrio spp. have been implicated as being one of the major causes of the disease problems in penaeid shrimp culture. For many pathogens, the outcome of the interaction between host and bacterium is strongly affected by the bacterial population size. Efforts for rapid molecular detection is to isolate specific genes which was owned by the pathogenic luminescent Vibrio and used it as the marker molecular in the rapid diagnosis of the disease. Haemolysin and gyrase gene known as one of the specific genes possessed pathogenic bacteria including Vibrio. The objectives of this study were to determine the specificity of designed specific primer from local isolate. Specificity test was performed for haemolysin primer (IAVhF1 and IAVhR1), gyrase primer (IAGyrF1 and IAGyr2) and commercial detection kit. The specificity test comparing the PCR result of pathogenic Vibrio DNA template as positive control, and DNA template from non pathogenic luminescent Vibrio, as well as pathogenic non Vibrios. The result showed that at annealing temperature of 60°C, haemolysin primer was more specific in detecting pathogenic Vibrio for penaeid shrimp than that of gyrase primer and commercial detection kit.
Co-Authors Endang Susianingsih Ince Ayu Khairana Kadriah Muharijadi Atmomarsono Munti Yuhana