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Ketut Suwirya

Research Institute for Mariculture, Gondol, Bali

Author-ID : 3672564

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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APPARENT DIGESTIBILITY OF SELECTED FEED INGREDIENTS IN DIETS FOR MUD CRAB (Scylla paramamosain) Ketut Suwirya; Nyoman Adiasmara Giri; Muhammad Marzuqi; Sophia Lasma Sagala
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Cultured mud crabs (Scylla spp.) are commonly fed with ‘trash’ fish. Insufficient supply, high cost and variable quality of ‘trash’ fish has lead to a need to develop costeffective and environmentally friendly formulated diets. This study was conducted to determine quality of selected feed ingredients as protein sources in mud crab diets based on their nutrient composition and digestibility coefficients for dry matter (ADMD), crude protein, lipid and energy. The digestibility coefficients for ADMD ranged from 82.46% to 89.20%. Animal-based feedstuffs such as shrimp head, tiny shrimp and squid liver meal had higher ADMD values than fish meal. Of the plant-based feedstuffs, soy bean meal had the highest ADMD values (89.20%) and corn gluten had the lowest (82.46%). Corn gluten had the lowest protein digestibility (78.81%) and soy bean meal had the highest (96.05%). The lowest energy digestibility (71.13%) was obtained in corn gluten meal. Soy bean meal had a higher energy digestibility value (98.48%) than fish meal (85.95%). All animal meal sources had similar energy digestibility values (85.86%—92.09%).

THE EFFECTS OF DIFFERENT LEVELS OF DIETARY PROTEIN AND LIPID ON THE GROWTH OF RED SNAPPER, Lutjanus sebae Nyoman Adiasmara Giri; Ketut Suwirya; Muhammad Marzuqi; Ni Wayan Widya Astuti
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Red snapper, Lutjanus sebae is favored in mariculture activities because it has a relatively good market and price. Technology for big scale seed production of this species has been developed and is now adequate to supply seed for grow-out activities. However, the availability of artifical diets for L. sebae is still a major constraint for grow-out production. Data on optimum dietary protein and lipid requirements for this fish as a basic information in feed development is not available yet. The objective of the present study was to find out dietary protein and lipid requirements for juvenile of L. sebae. A 70-day feeding experiment was conducted in 24 fiberglass tanks, 200 L volume. Each tank was equipped with a flow-through water system. Twenty five hatchery-produced juveniles of L. sebae (43.1 g BW) were randomly selected and stocked in each tank. The fish were fed with the experimental diets twice everyday at a level of 3% of biomass for the first 4 weeks, and then 2% of biomass afterward. Twelve experimental diets were prepared in form of dry pellet containing casein and fish meal as the main protein sources. Experimental diet had 4 levels of crude protein (32%, 37%, 42%, and 47%) and each protein level consisted of 3 levels of lipid (7%, 12%, and 17%). The experiment employed factorial method with completely random design using 12 combination treatments and 2 replications for each treatment. Result of the experiment showed that there was no significant effect of dietary protein and lipid on growth, feed consumption, and feed efficiency of tested fish. Growth and feed efficiency of fish fed on diet containing 42% and 47% crude protein were significantly higher than that of fish fed on diet containing 32% and 37% crude protein. High lipid content in the diet (17%) resulted in poor growth and poor feed efficiency. This data indicates that Lutjanus sebae has limited ability to utilize dietary lipid as an energy source. Result of the present study recommends that dietary protein and lipid requirement for good growth of L. sebae should be 42% and 12%, respectively.

REPLACEMENT OF FISH MEAL PROTEIN BY SOY BEAN AND CORN GLUTEN MEAL PROTEINS IN THE DIET OF MUD CRAB, Scylla paramamosain Ketut Suwirya; Nyoman Adiasmara Giri; Muhamad Marzuqi
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Mud crab culture relies heavily on trash fish as the main source of feed ingredients. Artificial diets have been developed for mud crab and most of them have high content of fish meal. The increasing cost and demand of fish meal has encouraged feed manufacture to search for cheaper alternative protein sources such as plant protein. There is an urgent need to find suitable alternative protein sources to reduce the dependence of fish meal in mud crab diet. The objective of this study was to develop compounded feeds for juvenile of mud crab with reduced fish meal content, and as an alternative of trash fish feeding. For that reason, the experiment was done. Experimental diets were fish meal, 20% of soy bean (20% SBP), 40% of soy bean (40% SBP), 20% of corn gluten (20% CGP), and 40% of corn gluten meal protein (40% CGP). Average initial mud crab body weight of 0.65 ± 0.03 g was fed experimental diets for 56 days. The result showed that dietary fish meal protein can be replaced by 20% of soy bean and 20%–40% of corn gluten proteins for mud crab (Scylla paramamosain) diet. Thus, it can arguably be concluded that soy bean and corn gluten proteins are the alternative protein sources to partially replaced fish meal.

PATHOGENECITY OF GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV: Megalocytivirus, FAMILY Iridoviridae) TO CORAL TROUT GROUPER Plectrophomus leopardus Ketut Mahardika; Ahmad Muzaki; Ketut Suwirya
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Grouper sleepy disease iridovirus (GSDIV), a member of the genus Megalocytivirus in the family Iridoviridae, has been known to cause large scale mortalities resulting in severe economic losses in grouper industries in south-east Asia including Indonesia. In this study, experimental infection of coral trout grouper Plectrophomus indicus with GSDIV was performed to evaluate the viral pathogenecity to this fish species. After virus exposure, the mortalities of coral trout grouper injected with primary and 10-1 dilution of spleen homogenates derived from tiger grouper Epinephelus fuscoguttatus were 100% and 90%, respectively. Histopathology revealed that moribund fish receiving GSDIV inoculum displayed massive formation of enlarged cells in the spleen and hematopoitic tissues. Under electron microscopy, the enlarged cells were observed as inclusion body bearing cells (IBCs) and necrotic cells allowing virus propagation within an intracytoplasmic virus assembly site (VAS). GSDIV virions were 167-200 nm in size. These findings confirmed that GSDIV has severe pathogenicity to coral trout grouper and IBCs as well as necrotic cells were determined to be the pathognomonic sign of megalocytivirus-infected coral trout grouper.

Co-Authors Ahmad Muzaki Ketut Mahardika Muhamad Marzuqi Muhammad Marzuqi Ni Wayan Widya Astuti Nyoman Adiasmara Giri Sophia Lasma Sagala

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