Rosmiati Rosmiati
Balai Riset Perikanan Budidaya Air Payau dan Penyuluhan Perikanan, Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

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EFFECT OF VARIOUS CRYOPROTECTANTS ON PRESERVATION OF BLACK TIGER Penaeus monodon SHRIMP SPERMATOZOA Rosmiati Rosmiati; Ike Trismawanti; Samuel Lante
Indonesian Aquaculture Journal Vol 15, No 2 (2020): (December, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.2.2020.77-83

Abstract

The development of cryopreservation technique on tiger shrimp Penaeus monodon broodstock spermatophore has been carried out to support the artificial insemination. This study aims to determine the effect of three cyoprotectants (methanol, dimetylsulphoxide (DMSO), and glycerol) for long term storage of tiger shrimp Penaeus monodon spermatozoa. Spermatophores were collected from the wild broodstocks through electrical shock. Spermatozoa were obtained by homogenizing the spermatophores using a Radnoti micro homogenizer in Ca-free saline solution containing one of three cryoprotectans (methanol, dimetylsulphoxide, and glycerol) separately at the concentration of 5%. One mL of each cryoprotectant containing spermatozoa with the density of 1.02 x 106 cell/mL was transferred into a cryovial and cryopreserved at room temperature, -20°C and -196°C for 5, 10, and 30 days. The apparent sperm viability (ASV) of cryopreserved spermatozoa was monitored after treated. Thawing of cryopreserved spermatozoa was carried out in a 30°C wáter bath for two minutes. The result showed that the best apparent sperm viability was obtained at the using of glycerol at -196°C in liquid nitrogen, even after the thirty days of cryopreservation time period with the ASV of 0.82 x 106 cells/mL (80.39%). Meanwhile two other cryoprotectans displayed the ASV of 0.54 x 106 cells/mL (56.86%), and 0.23 x 106 cells/mL (22.55%). for DMSO and methanol, respectively. In turn, the control showed the lowest ASV with the ASV of 0.01 x 106 cells/mL (1.27%). The ASV showed by this glycerol exhibited asignificant difference (P<0.05) to that of methanol, DMSO, and control.
PERFORMA BIBIT RUMPUT LAUT Gracilaria verrucosa HASIL KULTUR JARINGAN DENGAN BUDIDAYA METODE SEBAR (BROADCAST) DI TAMBAK KABUPATEN SINJAI Rosmiati Rosmiati; Harlina Harlina; Emma Suryati; Rohama Daud; Herlinah Herlinah
Jurnal Riset Akuakultur Vol 14, No 3 (2019): (September, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.265 KB) | DOI: 10.15578/jra.14.3.2019.145-152

Abstract

Rumput laut Gracilaria verrucosa asal Kabupaten Sinjai memiliki kualitas paling rendah di antara semua sentra produksi Gracilaria sp. di Sulawesi Selatan. Hal ini salah satunya dikarenakan oleh bibit yang buruk. Penyediaan benih rumput laut yang berkualitas dapat dilakukan salah satunya dengan penggunaan bibit hasil kultur jaringan. Perbanyakan bibit Gracilaria verucosa dapat dilakukan dengan menggunakan metode tali panjang long line maupun metode sebar (broadcast) di tambak. Tujuan penelitian ini untuk mengetahui respons pertumbuhan, kandungan agar, dan kekuatan gel (gel strength) dari bibit G. verucosa hasil kultur jaringan di tambak Kabupaten Sinjai. Metode penelitian ini adalah eksperimen dengan dua perlakuan dan tiga ulangan yaitu perlakuan A (bibit kultur jaringan) dan B (bibit lokal) dengan berat awal masing-masing 10 kg. Pemeliharaan bibit dengan metode sebar dilakukan selama 30 hari. Hasil analisis ragam menunjukkan bahwa laju pertumbuhan harian (DGR), kandungan agar dan gel strength bibit kultur jaringan dan bibit lokal menunjukkan perbedaan yang nyata (P<0,05). Secara kuantitas hasil produksi bibit hasil kultur jaringan memiliki pertumbuhan yang lebih tinggi daripada bibit lokal dengan berat akhir bibit 44,3 ± 4,16 kg hasil kultur jaringan dan 33,0 ± 4,35 kg lokal dengan DGR 4,97% bobot/hari (kultur jaringan) dan 3,90% bobot/hari (lokal). Secara kualitas bibit hasil kultur jaringan lebih baik dari bibit lokal, ditunjukkan dengan persentase kandungan agar bibit hasil kultur jaringan lebih tinggi daripada bibit lokal dengan rendemen agar 22,19 ± 2,45% (kultur jaringan) dan 16,50 ± 0,96% (lokal), sementara gel strength sebesar 204,20 ± 0,45 g/cm2 (hasil kultur jaringan) dan 128,10 ± 1,55 g/cm2 (bibit lokal).Seaweed Gracilaria verrucosa from Sinjai Regency has the lowest quality among all Gracilaria sp. Production centers in South Sulawesi due to the low quality of the seed. The seed quality can be improved using seed selection, followed by tissue-culture methods. Long-line and broadcast methods in brackishwater ponds are the efficient seaweed culture techniques to multiply the number of Gracilaria verrucosa seeds. This research was aimed to determine growth performance, gel content, and gel strength of seeds produced from tissue-culture and local seaweed farming. The experiment consisted of two treatments: treatment A (cells culture seed) and B (local seed) with the initial weight of 10 kg, each has three replicates. Both seeds were stocked and reared in the ponds using the broadcast method for 30 days. The results of DGR, gel content and gel strength showed a significant difference between tissue-cultured and local seeds (P<0.05). The tissue-cultured seed had better growth than the local seed with 4.97% mass/day for tissue-cultured seed and 3.90 mass/day for local seed. The tissue-culture seed also had better quality in agar content and gel strength. The agar content of tissue-cultured was 22.19 ± 2.45% and the local was 16.50 ± 0.96%. The gel strength of tissue-culture was 204.20 ± 0.45 g/cm2, and the local was 128.10 ± 1.55 g/cm2.