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ERMIN KATRIN W.
LABORATORIUM BAHAN KESEHATAN PATIR-BATAN
Medicine & Pharmacology

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Uji Aktivitas Inhibisi Isolat Kuersitrin dari Benalu Masisin Dendrophthoe pentandra (L.) Miq. terhadap Sel Leukemia L1210 ERMIN KATRIN W.; RISMA MARISI TAMBUNAN; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 1 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Parasitic plant [Dendrophthoe pentandra (L.) Miq.] on the masisin [Rhodomyrtus tomentosa (Aiton) Hassk.] was traditionally used for cancer treatment in Central Kalimantan. Maceration of dried Dendrophthoe pentandra using ethanol followed by ethyl acetate produced ethanol and ethyl acetate extracts which exhibit inhibitory activity against leukemia L1210 cells with IC50 of 17.3 and 20.9 µg/mL, respectively. Fractionation of ethyl acetate extract by column chromatography yielded 7 fractions (F1~F7). Inhibitory activity test on fractions F1~F7 against leukemia L1210 cells showed inhibitory activity with IC50 0f 19.9, 19.0, 20.7, 16.0, 13.7, 12.5, and 17.4 µg/mL, respectively. During evaporation of F5 by rotary evaporator, a yellowish precipitate was observed and identified as quercitrin (quercetin 3-O-rhamnoside). Inhibitory activity test on quercitrin against leukemia L1210 cell showed that the crystal has an IC50 of 14.7 µg/mL. Separation of F7 by semipreparative HPLC reverse phase column yielded isolate A and B which exhibited inhibitory activity on leukemia L1210 cells with IC50 of 15.0 and 14.4 µg/mL, respectively. Subsequent solvent partition of ethanol extract into ethyl acetate-water and separation of ethyl acetatesoluble portion by column chromatography produced 7 fractions (F8~F14). Further separation of F14 by column chromatography yielded quercitrin and isolate C which has not been identified yet.
Sitotoksisitas terhadap Sel Leukemia L1210 dan Profil Kromatogram dari Serbuk Temu Putih Curcuma zedoaria (Berg) Rosc. yang Diradiasi ERMIN KATRIN W.; JOHANS RICHARD ALBERT; SWASONO R. TAMAT; SUSANTO SUSANTO; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 1 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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White turmeric Curcuma zedoaria (Berg) Rosc. is useful for treating cancer disease. It is very susceptible to microbial, therefore gamma irradiation technique to reduce microbial contamination is necessary in order to extend the storing period. Gamma irradiation on white tumeric was carried out by cobalt-60 source at doses of 5, 7.5, 10, and 15 kGy. Then they gradually were maeerated with n-hexane, ethyl acetate, and ethanol. The purpose of this research was to study the effect of gamma irradiation by observing parameters on the cytotoxic activity of ethyl acetate extract and active fraction of white tumeric rhizome against L1210 leukemia cells, also profiles of thin layer and HPLC chromatograms of active fraction as anticancer agent. Ethyl acetate extract of irradiated and control samples were the most active to inhibit the growth of L1210 leukemia cells with IC50 value of 4.71 ug/mL. Ethyl acetate extracts from irradiated and control samples were fractionated using column chromatography and obtained 7 fractions (Fr), respectively. Fraction 3 of control sample was the most active fraction with IC50 values 1.43 µg/ ml. The IC50 value of Fr 3 decreased with increasing irradiation doses and chromatogram profiles of radiated samples with doses of > 5 kGy were changed. The maximum radiation dose for white turmeric preservation is 7.5 kGy, at this dose its anticancer efficacy was maintained.
Iradiasi Sediaan Obat Herbal Temu Putih Curcuma zedoaria (Berg) Rosc.: Cemaran Mikroba, Sitotoksisitas dan Profil Kromatogram ERMIN KATRIN W.; EPSI NARULITA; ZUHELMI AZIZ; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Gamma irradiation has been used for preservation of herbal medicines, one of which is temu putih Curcuma zedoaria (Berg) Rosc. to cure cervical cancer. This research aimed to study the gamma irradiation effects on microbial contamination, cytotoxic activity against leukemia L1210 cell lines and chromatogram profile of the temu putih active fraction. Gamma irradiation was performed with a 60Co source at 5, 7.5, 10 and 15 kGy. After irradiation at > 7.5 kGy bacteria and mould were not present on herbs. Control and irradiated samples were consecutively macerated with n-hexane, ethyl acetate and ethanol. Ethyl acetate extract was the most cytotoxic gainst L1210 leukemia cells with an IC50 value of 10.60 μg/mL as compared to n-hexane or ethanol extract. Ethyl acetate extract was fractionated with column chromatography producing six fractions. Fraction 2 was the most cytotoxic against L1210 leukemia cells with an IC50 value of 2.44 μg/mL. The thin-layer chromatographic analysis results of ethyl acetate extract and fraction 2 of control and irradiated samples showed the presence of curcumin and chromatographic pattern similar to the control. Curcumin contents in fraction 2 were between 35 to 51%, which were not significantly changed between samples, although was irradiated up to 15 kGy. The maximum dose for irradiation of temu putih herbal preparation is 15kGy.
Co-Authors EPSI NARULITA HENDIG WINARNO Hendig Winarno JOHANS RICHARD ALBERT Risma Marisi Tambunan SUSANTO SUSANTO Swasono R. Tamat Zuhelmi Aziz