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SUGENG RIYANTO
UNIVERSITAS GADJAH MADA
Medicine & Pharmacology

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Pengaruh Lupene-ol dan Lupene-on dari Aegle marmelos Correa terhadap Pelepasan Enzim β-hexoaminidase dari Sel Mast AGUNG ENDRO NUGROHO; SUGENG RIYANTO; MOHAMAD ASPOLLAH SUKARI; KAZUTAKA MAEYAMA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 1 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

20(29)-lupene-3α-ol and 20(29)-lupene-3-on are pentacyclic triterpenes isolated from Aegle marmelos Correa collected in Yogyakarta, Indonesia. Their molecular structures were confirmed in Universiti Putra Malaysia. These compounds were obtained from petroleum ether extract of the plant’s stem bark. This study investigated the effects of the compounds on the β -hexoaminidase enzyme release from mast cell culture (RBL-2H3 cell line). DNP24-BSA was used as immunologic inducer for the β -hexoaminidase release from the mast cells. The enzyme release was determined by colorimetric methods with a substrate, p-nytrophenyl-2-acetamydo-2-deoxy- β -D-gluko-pyranocide, and a microplate reader at 405 nm. lt was found that treatment with 20 µg/ml DNP24-B SA could stimulate the release of β -hexoaminidase from RBL-ZH3 cells by 3O.83 ± 1.10%. Lupene-ol and lupene-on showed inhibitory effects on the β -hexoaminidase release from RBL-2H3 cells induced by DNP24-BSA in dose-dependent manner. At the dose of 100 µM, lupene-ol and lupene-on inhibited the β -hexoaminidaserelease by 35.69i4.7 5% and 39.19 9.56%, respectively. The ICN values of their effects on DNP24-BSA experiments were 59.40 µM and 72.51 µM. The results suggested that the inhibitory effect of lupene-ol and lupene-on on the β-hexoaminidase release involved mechanisms related to the interaction of IgE on the RBL-2H3 cells surface or intracellular signal transductions found in the mast cell degranulation. Key words: Aegle marmelos Correa, lupene-ol, lupene-on, mast cells, β -hexoaminidase enzyme.
Penangkapan Radikal 2,2-Difenil-1-Pikril Hidrazil oleh Ekstrak Buah Psidium guajava. L dan Averrhoa carambola L. ABDUL ROHMAN; SUGENG RIYANTO; RIZKA DAHLIYANTI; DIMAS BAGUS PRATOMO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 7 No 1 (2009): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Two fruits, Guava (Psidium guajava L.) and Carambola (Averrhoa cammbola L.), Which are frequently consumed by Indonesian people, have been evaluated for its activities as 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The fruits were macerated, evaporated, dissolved in Water, partitioned with chloroform and then with aethyl acetate to afford methanolic extract and fractions of chloroform, aethyl acetate, and Water respectively. Total phenolic and total flavonoid contents of each extract and fraction Were determined by spectrophotometric technique and used to evaluate the correlation between the natural chemical contents and their activities as DPPH radical scavengers. The results showed that extracts and fractions of R guajava L. fruit have more ability to scavenge 2,2-diphenyl- 1-picrylhidrazyl (DPPH) radical than those of A. carambola L. The total phenolic and total flavonoid contents of extracts and fractions of R guajava L. fruit are also higher than those of A. cammbola L.
Antagonisme Marmin terhadap Reseptor 5-Hidroksi Triptamin dan Reseptor Histamin pada Otot Polos Ileum Marmut AGUNG ENDRO NUGROHO; PUGU NOVI ARSITO; YANCE ANAS; JOKO TRI WIBOWO; SUGENG RIYANTO; MOHAMAD ASPOLLAH SUKARI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 2 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The exploration of several active compounds of medicinal plants Aegle marmelos Correa (Rutaceae) have found marmin that strongly inhibit the histamine release from mast cells. The present study investigated the antagonism phenomena of marmin on 5-hydroxy triptamine and histamine receptors in guinea-pig ileum smooth muscle. The study was performed using isolated-organ technique in Tyrode buffer medium. The agonists used to sensitize the receptors were histamine and 5-hydroxy triptamine. The type of antagonism was identified by considering: 1). the decrease of pD2 or Emax values of agonists due to pretreatment of marmin, and 2) Schild regression method. In the study, marmin (10 and 100 mM) decreased the maximum contraction effect of 5-HT by 40% and 60%, respectively. However, marmin did not influence the pD2 value of 5-HT . On the other side, marmin did not influence the maximum contraction effect of histamine, however, decreased the pD2 value of histamine. Based on Schild regression method, the pA2 value of marmin was 0.8016. In conclusion, marmin non-competitively antagonized 5-HT 4 receptor, and competitively antagonized histamine receptor in ileum smooth muscle.
Co-Authors ABDUL ROHMAN Agung Endro Nugroho DIMAS BAGUS PRATOMO JOKO TRI WIBOWO KAZUTAKA MAEYAMA MOHAMAD ASPOLLAH SUKARI PUGU NOVI ARSITO RIZKA DAHLIYANTI Yance Anas