Nika Sari
Mahasiswa S1 Prodi PGSD, STKIP Bina Bangsa Getsempena

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Optimasi Jenis dan Konsentrasi ZPT dalam Induksi Kalus Embriogenik dan Regenerasi menjadi Planlet pada Carica pubescens (Lenne & K.Koch) Sari, Nika; R, Enni Suwarsi; -, Sumadi
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.3785

Abstract

Carica pubescens (Lenne & K. Koch) Badillo atau Vasconcellea pubescens, di Indonesia hanya ditemukan di daerah pegunungan Dieng dan Bali. Kultur jaringan merupakan teknik perbanyakan yang efisien. Tujuan dari penelitian ini adalah untuk mengkaji pengaruh jenis dan konsentrasi ZPT terhadap induksi kalus embriogenik pada media MS pada C. Pubescens. Penelitian pertama (induksi) merupakan percobaan 2 faktor yang disusun menggunakan rancangan acak petak tersarang (Split block Design) dengan 4 ulangan. Penelitian kedua (regenerasi kalus) merupakan percobaan satu arah yang disusun dengan menggunakan rancangan acak lengkap (RAL) dengan 4 ulangan. Analisis menggunakan ANAVA dan uji lanjut BNT dengan taraf kesalahan 5%. Hasil penelitian menunjukkan bahwa induksi kalus embriogenik dari eksplan jaringan daun paling tinggi didapatkan pada perlakuan ZPT 2,4-D 3 mg/l + TDZ 1 mg/l yang ditambahkan pada media MS, menghasilkan persentase kalus dan berat kalus yang paling tinggi. Untuk regenerasi jenis ZPT BAP dengan konsentrasi 4 mg/l dalam media MS arang aktif yang mengandung NAA 0,2 mg/l, menghasilkan persentase pembentukan kalus menjadi tunas dan jumlah kalus yang membentuk tunas dengan hasil yang optimal. Berdasarkan hasil penelitian disarankan untuk menginduksi kalus embriogenik menggunakan kombinasi ZPT 2,4-D dan thidiazuron, sedangkan untuk regenerasinya menggunakan ZPT BAP dan NAA.In Indonesia, Carica pubescens (Lenne & K. Koch) Badillo and Vasconcellea pubescens only found in mountainous areas in Dieng and Bali. Tissue culture is an efficient propagation technique. The aim of this research was to assess the effect of the type and concentration of plant growth regulator on embryogenic callus induction on MS medium in C. Pubescens. The first study (induction) was a two factor experiment were prepared using a nested plot randomized complete block design (Split block design) with 4 replications. The second study (callus regeneration) is a one-way experiment which prepared using a completely randomized design (CRD) with four replications. Data were analyzed using ANAVA and LSD to further test error level of 5%. The results showed that the induction of embryogenic callus from leaf tissue explants obtained at the highest 2,4-D treatment PGR 3 mg/l+TDZ 1 mg/l were added to the media MS, produce weight percentage of callus and callus highest. For regeneration of PGR BAP with a concentration of 4 mg/l in MS medium containing activated charcoal NAA 0.2 mg/l, a greater percentage of callus formation into buds and the number of callus forming buds with optimal results. Based on the research results, it suggested to induce embryogenic callus using PGR combination with 2,4-D and thidiazuron, while for regeneration was suggested to use PGR BAP and NAA.
Sistem Kata Sapaan Kekerabatan dalam Bahasa Melayu di Kepenghuluan Bangko Kiri Rokan Hilir Riau Sari, Nika; Ermanto, Ermanto; Nst., M. Ismail
Jurnal Pendidikan Bahasa dan Sastra Indonesia Vol 1, No 2 (2013): Seri G
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/1367-019883

Abstract

This article was written to (1) describing the form and used of greeting words in Melayu language based on lineage in Bangko kiri. (2) describing the form and used of greeting words in Melayu language based on marriage in Bangko Kiri. The data in this research consist of the idiom of language in sentence that followed by greeting words that’s belong there. The data has been collected by cakap method with pancing technique. The finding of this research is (1) the form and used of the greeting words based on patrilineal lineage, which are: there are sixteen form of greeting words that used for different ego. (2) Then there are twenty two the form of greeting words based on marriage line that has been use for different ego.