Sarsono Sarsono
Lab. Biokimia Fakultas Kedokteran Universitas Sebelas Maret Surakarta. Jl IR Sutami 36A Kentingan Jebres Surakarta

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Ekstrak Etanol Propolis Menurunkan Kadar IL-17 Serum pada Mencit Balb/C Model Asma Kronik Hadinoto, Sri Hartati; Sarsono, Sarsono; Aisyah, Siti; Prasetyo, Diding H.
Majalah Kedokteran Bandung Vol 45, No 4 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Interleukin-17 (IL-17) menginduksi perekrutan neutrofil ke dalam saluran napas dan berperan dalam patogenesis asma serta penentuan keparahan penyakit. Tujuan penelitian ini adalah menganalisis efek ekstrak etanol propolis (EEP) isolat Gunung Lawu terhadap kadar IL-17 serum pada mencit Balb/C model asma kronik. Penelitian ini merupakan penelitian eksperimental laboratoris dengan sampel 28 ekor mencit Balb/C jantan yang dibagi menjadi empat kelompok: kontrol, asma, asma+EEP, dan asma+antihistamin. Penelitian dilakukan di Laboratorium Histologi dan Biomedik, Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta, selama periode April-September 2012. Model asma kronik dibuat dengan menginduksi mencit Balb/C jantan menggunakan injeksi intraperitoneal (i.p.) ovalbumin (OVA, 100 μg/mencit) dan aluminium hidroksida (10%, 50 mg/mencit) dalam phosphate-buffered saline (PBS). Hewan coba disensitisasi selama 6 minggu dengan inhalasi 2% (b/v) OVA aerosol dalam NaCl 0,9% selama 30 menit/hari setiap 3 hari/minggu, untuk kelompok asma, asma+EEP, dan asma+antihistamin. Mencit kontrol tidak diinokulasi selama penelitian. Deteksi kadar IL-17 serum menggunakan enzyme-linked immunosorbent assay (ELISA). Uji kadar IL-17 serum menggunakan analysis of variance (ANOVA) dan untuk menentukan perbedaan kemaknaan digunakan p<0,05. Kadar IL-17 serum kelompok kontrol menunjukkan 17,15±8,25 pg/mL dan antihistamin 38,98±7,57 pg/mL. Ekstrak etanol propolis secara bermakna menurunkan kadar IL-17 serum (75,62±17,64 pg/mL vs 45,59±10,69 pg/mL, p<0,001) dibandingkan dengan kelompok asma. Simpulan, EEP isolat Gunung Lawu menurunkan kadar IL-17 pada hewan coba model asma kronik. [MKB. 2013;45(4):213–7]Kata kunci: Asma, interleukin-17 (IL-17), propolisEthanolic Extract of Propolis Decreases Serum IL-17 Levels in Balb/C Model of Chronic AsthmaInterleukin-17 (IL-17) induces the neutrophil recruitment into the respiratory tract while playing a role in the pathogenesis of asthma and determining the severity of disease. The aim of this study was to analyze the effect of ethanol extract of propolis (EEP) Lawu mountain isolates on IL-17 serum levels in Balb/C mice model with chronic asthma. This study was an experimental research laboratory with 28 male Balb/C mice divided into control, asthma, asthma+EEP and asthma+antihistamine groups. The study was conducted at the Histology and Biomedical Laboratory, Faculty of Medicine, Sebelas Maret University, Surakarta, during April to September 2012. A model of chronic asthma was induced in male Balb/C mice using an intraperitoneally (i.p.) injection ovalbumin (OVA, 100 μg/mice) and aluminum hydroxide (10%, 50 mg/mice) in phosphate-buffered saline (PBS). Experimental animals were sensitized for 6 weeks with inhaled 2% (w/v) OVA aerosol in 0.9% NaCl for 30 minutes/day, 3 days in a week, for asthma, asthma with EEP, and asthma with antihistamine mice model groups. Control mice were not inoculated during the study. Detection of IL-17 serum level was conducted by using enzyme-linked immunosorbent assay (ELISA). One way analysis of variance (ANOVA) for IL-17 serum level and p<0.05 were used to determine significant differences. Serum levels of IL-17 showed 17.15±8.25 pg/mL in control group and 38.98±7.57 pg/mL in antihistamine, respectively. Ethanol extract of propolis showed significant decreased in IL-17 levels (75.62±17.64 pg/mL vs 45.59±10.69 pg/mL, p<0.001) compared to asthma group. In conclusion, EEP Lawu mountain isolates can decrease IL-17 level in chronic asthma animal models. [MKB. 2013;45(4):213–7]Key words: Asthma, interleukin-17 (IL-17), propolis DOI: http://dx.doi.org/10.15395/mkb.v45n4.167
Antioxidant Effect of Bilimbi Fruit (Averrhoa Bilimbi Linn) Extract on SGPT Level in Mice Induced by Reheated Palm Oil Mulyono, Rosi Dwi; Putranto, R Prihandjojo Andri; ., Sarsono
Nexus Kedokteran Translasional Vol 5, No 2 (2016): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Introduction: Reheated palm oil was known to contain free radicals that cause damage and death of liver cell, antioxidant is needed to prevent. One source of antioxidant is Bilimbi fruit. This research aims to determine the effect of Bilimbi fruit extract and variant in dosage of Bilimbi fruit extract on levels of Serum Glutamic Piruvic Transaminase in mice induced by reheated palm oil. Methods: This research was a laboratory experimental with a post-test only control grup design. The subject of research was Swiss Webster mice, male, age 2-3 mounths, weight + 20 grams. The subject ware chosen by incidental sampling for 30 individuals, devided randomly into 5 groups, each groups consist of 6 mice. The control group (K) was given aquadest (0,3 ml/20 g BW and 0,2 ml/20 g BW). Mice in treated group 1 (P1) was given aquadest (0,3 ml/20 g BW) and reheated palm oil (0,2 ml/20 g BW). Mice in treated group 2 (P2), treated group 3 (P3) and treated group 4 (P4) was given Bilimbi fruit extract with dosage 5,6 mg/20 g BW; 11,2 mg/20 g BW; and 22,4 mg/20 g BW and reheated palm oil (0,2 ml/20 g BB). Bilimbi fruit extract was given 1 hour before reheated palm oil for 14 days. At the day-15, mice ware sacrificed and the animal blood was collected from pleksus retroorbitalis. The result was analyzed statistically by one way ANOVA and followed by LSD test (α =  0,05). Results: Mean of SGPT level in each group was K = 37,5 + 8,256 U/L; P1 = 50,92 + 8,267 U/L; P2 = 37,08 + 5,043 U/L; P3 = 43,34 + 7,834 U/L; P4 = 51,12 + 13,526 U/L. There wes significant difference between K – P1, K – P4, P1 – P2, and P2 – P4, there was not significant difference between K – P2, K – P3, P1 – P3, P1 – P4, P2 – P3, and P3 – P4. Conclusions: The extract of Bilimbi fruit decrease SGPT level in mice induced by reheated palm oil. An increase in dose of Bilimbi fruit extract cannot increase the influence to decrease SGPT level in mice induced by reheated palm oil. Keywords: Bilimbi fruit extract, SGPT, reheated palm oil, SGPT. 
ENGARUH EKSTRAK PATIKAN KEBO (EUPHORBIA HIRTA L.) TERHADAP HITUNG EOSINOFIL DARAH TEPI PADA MENCIT MODEL ASMA ALERGI Heri Prasetyo, Diding; Putranto, R.P. Andri; Martini, Martini; Sarsono, Sarsono
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.327 KB) | DOI: 10.33476/jky.v16i3.246

Abstract

Asthma is characterized by both chronic inflammation and remodeling of the airways. Eosinophils may contribute to inflammation associated with bronchial asthma by causing tissue damage and subsequently organ dysfunction, but also by the generation of cytokines and lipid mediators. It was reported in previous studies that pharmacological activities were identified in various compounds extracted fromEuphorbia hirta L. such as antiinflammatory,histamine inhibitor, lipoxygenase inhibitor, anti-allergic and cyclooxygenase inhibitor. This study was aimed to determine the effect of E. hirtaextract onperipheral blood eosinophils count in mice model of allergic asthma. MaleBalb/C mice were sensitized and challenged intraperitoneally (i.p) with ovalbumin(OVA). Mice were immunized i.p. on days 1 and 14 with 2.5 mgof OVA adsorbed to 7.75ml of Aluminium hydroxide gel. OVA challenges (10 mgOVA in l ml of PBS) were administered aerosolly on days 21, 23, 25 and 27, andperipheral blood eosinophilscount was determinedon day 28.A one-way analysis of variance (ANOVA) with LSDpost hocanalysis was used to determine significant differences. Results were expressed as mean+SEM, and values of p 0.05wereconsidered statistically significant. The results showed thatperipheral blood eosinophilscountin the OVA group was51.0+10.4cells/mm E. hirtaextract dose 500 mg/BW/day was30.2+6.3cells/mm3,E. hirtaextract dose 1,000 mg/BW/day was 30.4+6.0cells/mm and antihistamine group was32.2+8.6cells/mm respectively, whilecontrol mice showed46.6-8.8 cells/mm3. It was concluded that E. hirta extract could minimizeperipheralblood eosinophilscount.