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All Journal Integrated Lab Journal Tajdidukasi: Jurnal Penelitian dan Kajian Pendidikan Islam
Arifah Khusnuryani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Engineering Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Social Sciences Other

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PERAN LEMBAGA PENDIDIKAN MUHAMMADIYAH DALAM PENDIDIKAN SAINS TERINTEGRASI AGAMA DAN ETIKA Arifah Khusnuryani
Tajdidukasi: Jurnal Penelitian dan Kajian Pendidikan Islam Vol 2, No 2 (2010)
Publisher : Muhamadiyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47736/tajdidukasi.v2i2.7

Abstract

Science and technology have been integrated into almost every aspect of human life. The development of science-technology raise a dilemma, in one hand it gives many benefits that can improve the quality of human life, but on the other hand it also has some disadvantages related to the technology itself and the ethical aspect of the development of science-technology. Therefore, we need an education about wisdom in developing science-technology through religion education or other disciplines which deliver ethics or values. Muhammadiyah is one of Islamic social-society board which has some institution of education, from early stage until higher education. Those institutions of education in Muhammadiyah have an important and strategic role in delivering education of science-technology which is integrated with religion and ethics, and vice versa. Ethics education, especially bioethics, usually is delivered for medical students. However, there is a thought that this ethics education should be delivered to early stage until higher education. Therefore, Majelis Pendidikan Dasar dan Menengah and Majelis Pendidikan Tinggi Muhammadiyah has important role in formulating and developing the education of science-technology which is integrated with religion and ethics. Then the education institution of Muhammadiyah can produce some scientists who competence in their disciplines which is based with religion and ethics values. Key Words: Science-Technology, Religion, Ethics and Muhammadiyah
ISOLASI DAN ANALISIS KUALITAS DNA PLASMID (pGEM®-3Zf(+)) SEBAGAI SEDIAAN KEBUTUHAN PRAKTIKUM DI LAB. BIOLOGI FST Arifah Khusnuryani; Jumailatus Solihah; Anif Yuni Muallifah
Integrated Lab Journal Vol. 4 No. 1 (2016)
Publisher : UIN Sunan Kalijaga Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.89 KB) | DOI: 10.14421/ilj.2016.%x

Abstract

Implementation of practical work in biology laboratory needs supporting media including laboratory equipments and materials. In genetics and molecular biology, the routine materials are mostly molecular resources, which are produced by certain company. To get the materials, we must order and pay for a relative high price.These molecular resources in biology laboratory is not only beneficial for practical activity, but also useful for research activity in related fields.This research works as an application of methods applied in laboratory to result in a product that is valuable in practical works, i.e. the pGEM plasmids. The methods that were employed involved the transformation of E. coli with pGEM plasmid, blue-white screening by using X-gal, and plasmid isolation by alkaline lysis method. The transformation results showed a relative low efficiency at 1,2 x 103cfu/µg DNA. The electrophoresis in 0.8% agarose gel demonstrated that the plasmid DNA was successfully isolated with some thin bands at position 3000-4000bp, despite of a large chromosomal DNA and some smear RNA were still observed. The DNA obtained is pure enough, characterized by the ratio of A260/280 at the range of 1,8-2,0.
Co-Authors Anif Yuni Muallifah Jumailatus Solihah