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All Journal Jurnal Natur Indonesia Biosaintifika: Journal of Biology & Biology Education
Petrus Hary Tjahja Soedibja
Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

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Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning Kusbiyanto, Kusbiyanto; Soedibja, Petrus Hary Tjahja; Nuryanto, Agus; Qonita, Nael Huda; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9301

Abstract

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome b-AluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.
Phylogeography and Genetic Diversity of Humpback Grouper Cromileptes altivelis based on Cytochrome C Oxidase I Susanto, Agus Hery; Nuryanto, Agus; Soedibja, Petrus Hary Tjahja
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.477 KB) | DOI: 10.31258/jnat.14.1.47-51

Abstract

Humpback grouper is one of the most popular fish group in the international live trade in Asia-Pacific regions. The price for one kilogramlive of humpback grouper, especially in Spermonde Archipelago South of Sulawesi, is range from 350.000-400.000 IDR, whereas in theretail level in Hong Kong the price was about 92 US$. This condition leads to the reduction of nature population due to overexploitation.Population decreasing due to overexploitation may cause loss of genetic diversity within population and lead to reduce of potentialadaptive, population resistance, and productivity. Therefore, it is important to do some efforts to avoid adverse effect of overexploitationon humpback grouper population in Indonesia. One of the valuable efforts is providing genetic information such as phylogeography andgenetic diversity of humpback grouper Cromileptes altivelis. Analysis was based on 618 base pairs fragment of cytochrome c oxidase I genefrom 36 individuals (sequences) of Cromileptes altivelis collected at four different sites (e.g. Pulau Seribu, Jepara, Situbondo and SpermondeArchipelago). The results showed that humpback grouper population has a high haplotype and nucleotide diversity. However, high geneticdiversity and polymorphisms could not reveal population fragmentation (Φ stt = 0.000). It is suggested that high gene flow rather thanpopulation sub structuring was occurred. High level genetic diversity and polymorphisms are vital related to adaptive potential toenvironmental alteration.
Co-Authors AGUS HERY SUSANTO Agus Nuryanto Hendro Pramono Kusbiyanto Kusbiyanto, Kusbiyanto Nael Huda Qonita