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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati INDONESIAN JOURNAL OF PHARMACY
C. J. Soegihardjo
Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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PROTOPLAST SUSPENSION CULTURE OF LEAVES MESOPHYLOF Centella asiatica (L.) Urban AND QUALITATIVE ANALYSIS ASIATICOSIDE Prihastanti, Erma; Soegihardjo, C. J.; Purbaningsih, Susiani
Indonesian Journal of Pharmacy Vol 12 No 1, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.652 KB) | DOI: 10.14499/indonesianjpharm0iss0pp10-19

Abstract

Centella asiatica leaves has been used as diuretic, antihypertensi, anti leprae, skin infection, burning skin and celloid. Cells suspenson culture of mesophyll of Centella asiatica leaves were carried out in three steps: isolation, purification, and cell culture. The aim of this research is to investigate the influences of leaves position (age of leaves) and concentration of macerozyme R-10 for cells number and viability, and the influence of sucrose concentration for cells growth and biosynthesis of asiaticoside. Isolation and purification of mesophyll cell has been performed using factorial completely randomized design. The first factor was leaf position (age of leaves), and the second factor was concentration of macerozyme. Collected data were analysed using Anova and Duncan’s test at 0.0 – 2.5 – 5.0 and 7.5 per cent respectively. Asiaticoside produced by cell biomass were analyzed qualitatively using thin layer chro- matography. The result revealed that mesophyll cells could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cells isolated from second leaves treated with 0.1% macerozyme (1.32 . 107 cells/ml). Addition of sucrose to suspension culture medium produced higher packed cells volume (pcv) percentage than no sucrose was added, (0% sucrose gave pcv 8.5%, 2.5% sucrose pcv 22%, sucrose 5% pcv 21.5%, and 7.5% sucrose pcv 15.75%). The asiaticoside production not only depended on sucrose, because without any sucrose was added, the asiaticoside was also available..Key Words: Cell suspension culture, mesophyll, Centella asiatica (L.) Urban, asiaticoside
ISOLASI DAN IDENTIFIKASI STREPTOMYCETES DARI RIZOSFER JAGUNG (ZEA MAYS L.) YANG BERPOTENSI SEBAGAI PENGHASIL ANTIBIOTIKA Ambarwati, Ambarwati; Soegihardjo, C. J.; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.332 KB) | DOI: 10.24002/biota.v15i1.2639

Abstract

In attempt to understand the diversity of Actinomycetes that is potential to be antibiotic producer, Streptomycetes were isolated and identified from soil sample taken from rhizosphere and non-rhizosphere of corn (Zea mays L.). The best antibiotic producers were identified by Scanning Electron Microscopy analysis and the identification of antibiotic produced conducted by using Thin Layer Chromatography analysis. The result of the study showed that 58 isolates were assigned to 17 colour groups. Ten isolates among the representatives of 17 colour groups were found potential to be antibiotic producer. Four isolates out of 10 isolates could inhibit both Staphylococcus aureus ATCC 25923 and Bacilus subtilis FNCC 0060, one isolate could inhibit only Staphylococcus aureus ATCC 25923 and five isolates could inhibit only Bacilus subtilis FNCC 0060. But no isolate could inhibit Escherichia coli ATCC 35218 and Salmonella typhimurium FNCC 0164. Among 10 isolates of antibiotic producer it was found that only one isolate (RNJ14) could strongly inhibit Staphylococcus aureus ATCC 25923 with inhibition zone diameter of 32.33 mm. On the bases of Thin Layer Chromatography analysis, the antibiotic produced by the isolate RNJ14 was identified to be lincomycin. Therefore it could be concluded that streptomycetes isolated from the rhizosphere and non-rhizosphere of corn (Zea mays L.) were potential to produce antibiotic.
Co-Authors Ambarwati Erma Prihastanti LANGKAH SEMBIRING Susiani Purbaningsih, Susiani