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Soeyoko Soeyoko
Fakultas Kedokteran Universitas Gadjah Mada
Immunology & microbiology Neuroscience

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Spesifisitas antibodi monokional anti protein ekskretori-sekretori Brugia malayi terhadap protein nematoda lain Soeyoko, Soeyoko
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 02 (1999)
Publisher : Universitas Gadjah Mada

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Abstract

Background: Previous studies using hybridoma technique had produced monoclonal antibodies against excretory-secretory (ES) protein of B. malayi i.e. Fesi, Fes3, Fes7, Fesl3 and Fesi5. Those monoclonal antibodies will be used to diagnose malayan filariasis in the endemic areas.Objectives: To reveal binding specificities of those monoclonal antibodies to ES protein of B. malayi, heterologous protein i.e. Ascaris lumbricoides, Necator americanus and homologous protein i.e. Brugia pahangi.Methods: Binding specificities of those monoclonal antibodies to other nematodes protein were assessed by ELISA and inhibition ELISA techniques.Result: Those monoclonal antibodies showed different binding specificities profiles. Fesi, Fesi3 and Fes. revealed specific reaction to ES protein of B. malayi but they did not react to heterologous and homologous protein. Fes3 and Fes7 did not reveal binding specificities to heterologous protein but they had low cross-reactivity to homologous protein. Cross-reactivity values of Fes3 and Fes7 were 33.3% and 22% respectively. Conclusion: Monoclonal antibodies against ES protein of B. malayi i.e. Fesi, Fesi3 and Fesl5 revealed specific reaction to ES protein of B. malayi, but Fes3 and Fes7 had low cross-reactivity to protein of B. pahangi.Key words: binding specificity - monoclonal antibody - ES protein - heterolog protein - homolog protein - ELISA.
Diagnosis filariasis malayi dengan ELISA Sandwich menggunakan antibodi monokional Soeyoko, Soeyoko
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 02 (1996)
Publisher : Universitas Gadjah Mada

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Malayan filariasis is still the major public health problem in Indonesia. In endemic area, when microfilaremia and clinical manifestations are absent, the diagnosis of malayan filariasis is extremely important, particularly for filariasis control.With the development of hybridoma technology, it is possible to produce monoclonal antibody against any stage of Brugia malayi, which is capable of detecting the circulating parasite antigen in malayan filariasis sera.Sandwich ELISA, assay using Fab 4 monoclonal antibody could identify circulating parasite antigen in human sera. With this assay it was found that: 100% of sera from symptomatic-microfilaremic, 50% of sera from symptomatic-amicrofilaremic, 82,1% of sera from asymptomatic-microfilaremic and 24,4% of sera from asymptomatic-amicrofilaremic residents of filariasis-endemic areas positively contained circulating antigen.In comparison with the conventional method, the sensitivity and specificity of this new technique are 85,2% and 75,4% respectively.From this study it is concluded that Sandwich ELISA is proved to be very sensitive as an immunological test to detect filarial infection without any clinical manifestation or microfilaria in the blood.Key words : malayan filariasis - circulating antigen - monoclonal antibody - Sandwich ELISA - asymptomatic-amicrofilaremic.
Detection of circulating antigen in sera of patients with malayan 1ilariasis by sandwich ELISA using monoclonal antibody Fes7 Soeyoko, Soeyoko
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 03 (1999)
Publisher : Universitas Gadjah Mada

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Background. The living lymphatic filarial worm Brugia malayi releases physiologically protein materials in the malayan filariasis patients which is called circulating antigen. Based on the clinical symptoms and the demonstration of microfilariae in the blood smears, people who are living in the endemic area can be classified into 4 groups: symptomatic microfilaraemic, symptomatic amicrofilaraemic, asymptomatic microfilaraemic and asymptomatic amicrofilaraemic.Objectives. To detect circulating antigen in sera of each group of patients with malayan filariasis in endemic area.Methods. Circulating antigen in sera was detected by sandwich ELISA using monoclonal antibody Fesi.Result. By sandwich ELISA using monoclonal antibody Fesi, 100% of symptomatic microfilaraemic sera, 35.7% of symptomatic amicrofilaraemic sera, 89.2% of asymptomatic microfilaraemic sera and 26.3% of asymptomatic amicrofilaraemic sera were positive for circulating antigen.Conclusion. Detection of circulating antigen in sera of malayan filariasis patients could be used to support the diagnosis of malayan filariasis in some cases which were difficult to be diagnosed by conventional method based on clinical symptoms and the demonstration of microfilariae in the blood smears.Keywords: circulating antigen - sandwich ELISA - monoclonal antibody - sera - Brugia malayi - asymptomatic amicrofilaraemic.
The Use of Monoclonal Antibody in the Detection of Circulating Antigen in Malayan Filariasis Cases: a Preliminary Report Soeyoko, Soeyoko
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 25, No 03 (1993)
Publisher : Universitas Gadjah Mada

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Filariasis (penyakit kaki gajah) di Indonesia disebabkan oleh cacing filaria. Wuchereria bancrofti, Brugia malayi dan Brugia tintori. Dan ketiga spesies tersebut, B. malayi merupakan penyebab utama filariasis terutama di daerah endemik di luar Jawa. Diagnosis filariasis sampai saat ini masih didasarkan atas hasil pemeriksaan klinis, parasitologis dan imunologis konvensional, namun ketiga cara tersebut banyak kelemahannya.Dalam era bioteknologi, dengan diternukannya teknik hibridoma; dapat dihasilkan antibodi monoklonal yang spesifik terhadap filaria B. malayi dan mampu mengikat circulating antigen dalam senim penderita filariasis.Dengan teknik pemeriksaan dot-blot didapatkan serum yang mengandung circulating antigen sebagai berikut: 75% pada serum kelompok penderita simtomatik-rnikrofilarernia; 40% serum kelompok penderita shntomatik-amikrofilaremia; 88,8% serum kelompok penderita asimtomatik-mikrofilarernia; dan 19,6% serum kelompok penderita asimtomatik-amikrofdarentia.Antibodi monoklonal dapat membantu diagnosis filariasis terutama pada yang asimtomat ik-amikrofilarem ia.Key Words: Brugia malayi- filariasis-filarial circulating antigen-antifilarial monoclonal antibodies-dot-blot assay
Characterization of a monoclonal antibody against surface coat of infective larvae of Brugia malayi. Soeyoko, Soeyoko
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 03 (1995)
Publisher : Universitas Gadjah Mada

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Abstract

Malayan filariasis is an important public health problem in Indonesia. A diagnosis of filariasis is normally based on clinical, parasitological and immunological examinations but the methods have limitations. There is an urgent need to improve immunodiagnostic techniques for malayan filariasis using monoclonal antibodies to detect circulating antigen. Recently, many types of monoclonal antibodies have been produced following immunization of mice with various stages of filarial antigens. Those monoclonal antibodies have different characteristics.The objective of this study was to explore the characteristic of specific monoclonal antibodies against the surface coat of infective stages of Brugia malayi larvae.This monoclonal antibody recognized antigen of 40.000 MW protein which is present among the antigens of surface coat of Brugia malayi larvae, as detected by an immunoblotting technique. Classification using EUSA techniques indicates that those antibodies are IgGi, IgG2b and IgG3 subclasses. In addition, these antibodies also demonstrate a low level reactivity with surface antigen of Brugia pahangi, Ascaris lumbricoides and hookwormKey words : surface coat - infective larvae - monoclonal antibody - circulating antigen - Brugia malayi
Co-Authors Adi Heru Sutomo Melianus Salakory, Melianus Sugeng Juwono Mardihusodo Sutanto Sutanto Thaharuddin Thaharuddin, Thaharuddin