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EFFECT OF CHITOSAN ON PROTEIN CONTENT IN THE MEDIUM CULTURE OF OSTEOBLASTS EXPOSED TO OXIDATIVE STRESS: EFEK KITOSAN TERHADAP KANDUNGAN PROTEIN DALAM KULTUR MEDIUM OSTEOBLAS YANG DIPAPAR DENGAN STRES OKSIDATIF Shanty Chairani; Sri Utami; Dewi Fatma Suniarti
Dentika: Dental Journal Vol. 16 No. 1 (2011): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.184 KB) | DOI: 10.32734/dentika.v16i1.1901

Abstract

Chitosan is a derivative of chitin which has potential for use in bone regeneration and has been reported can stimulate bone formation. Oxidative stress as one cause of bone damage, was found increased in osteoporosis, periodontitis and arthritis. One of the species oxygen reactive (ROS), hydrogen peroxide, has been reported can inhibit osteoblast proliferation. This study was aimed to investigate the effect of various chitosan concentrations on protein content in the culture medium of human osteoblast-like cell line, MG 63, which was exposed to hydrogen peroxide. MG 63 cells were exposed to various chitosan concentrations (% w/v) 0.1, 0.2, 0.4, and 1.6%. Culture cells without chitosan were used as a control. Cells were growth with α-MEM medium (37ºC, 5% CO2) until they became confluent, then they were exposed to hydrogen peroxide for 4 hours. The protein content in the culture medium was measured by using Bradford protein assay at 655 nm wavelength. The result showed that hydrogen peroxide decreased protein concentration in the medium culture compared with group without hydrogen peroxide. Treatment group with chitosan concentration 0.4% and 1.6% exhibited a significant increasing of protein concentration in osteoblast culture medium compared with control. In conclusion, in osteoblast culture medium chitosan can inhibit the decreasing of total protein concentration which was caused by oxidative stress.
DIALEKTOLOGI BAHASA MADURA DI DESA TAPAAN DAN DESA TLAGAH KECAMATAN BANYUATES KABUPATEN SAMPANG Fatana Auliyah; Sri Utami; Nuril Huda
Jurnal Sastra Aksara Vol 8 No 1 (2020): Jurnal Sastra Aksara
Publisher : Program Studi Pendidikan Bahasa Indonesia STKIP-PGRI Bangkalan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31597/jsa.v8i1.479

Abstract

Variations in languages found in Tapaan and Tlagah villages, both of theseareas are possible for data collection because these areas include coastal and mountainous areas which make a dialect factor. The formulation of the problem is how is the difference between Maduranese dialect in Tapaan village and Tlagah village in Banyuates subdistrict Sampang district? With the aim analyze the differences between Maduran dialect and Tapaan village, Tlagah village, Banyuates subdistrict, Sampang district. This research is a descriptive qualitative research with the power in the form of utterances or conversation which are then transcribed into written form in the form of words. The data is obtained from the original source of the study area in accordance with the data were researchers who were assisted by voice recording devices in the form of mobile phones. The data collection method in this research is the listening method and subsequent method uses a competent, free record, record method, record method, data transcription method, data transcription method, and translate data method. Next the researcher analyzes data that has been collected with the data element sorting technique, which is the matching method. Researchers also use data validity methods in the form of data validity methods, that is (a) prolong the participation of observations, (b) perseverance of observation, (c) tringualation. The results of this study obtained from research are the most common data in this study, namely the same word, but different in pronunciation, and there are also different words but the meaning is the same.
PEMULIHAN MINYAK SAWIT DARI SPENT BLEACHING EARTH DENGAN METODE EKSTRAKSI REFLUKS Muslich; Sri Utami; Nastiti Siswi Indrasti
Jurnal Teknologi Industri Pertanian Vol. 30 No. 1 (2020): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24961/j.tek.ind.pert.2020.30.1.90

Abstract

Spent Bleaching Earth (SBE) merupakan limbah hasil pemucatan minyak yang mengandung 20-40% minyak sehingga perlu dilakukan pemulihan. Tujuan penelitian ini untuk menganalisis pengaruh rasio bahan/pelarut terhadap rendemen dan mutu minyak serta mendapatkan perlakuan terbaik dalam proses ekstraksi minyak dari SBE. SBE yang digunakan berasal dari proses pemucatan crude palm oil (CPO) (bahan A) dan SBE dari proses pemucatan refined bleached deodorized palm oil (RDPO) yang telah disimpan selama tiga bulan (bahan B). Proses ekstraksi menggunakan metode refluks dengan pelarut heksan teknis selama 3 jam. Penelitian ini menggunakan rancangan acak lengkap faktor tunggal dengan perlakuan rasio bahan SBE/pelarut (1:4, 1:6, 1:8) (b/v). Hasil penelitian menunjukkan karakteristik sampel A memiliki kadar minyak 17,17%, kadar abu 31,27%, kadar volatil 33,49%, pH 3,21, dan kadar air 0,72%. Sampel B memiliki kadar minyak 38,28%, kadar abu 48,33%, kadar volatil 47,33%, pH 7,75, dan kadar air 1,00%. Rasio antara bahan sampel A terhadap volume pelarut mempengaruhi rendemen dan densitas minyak, sedangkan rasio antara bahan sampel B terhadap volume pelarut hanya mempengaruhi densitas minyak. Perlakuan terbaik untuk rasio bahan terhadap volume pelarut sampel A adalah 1:8 dengan menghasilkan rendemen minyak tertinggi 88,31%. Minyak yang dihasilkan memiliki bilangan asam 84,44 mg KOH/g, densitas 0,89 g/cm3, viskositas 4,91 cSt, dan bilangan iod 48,07 g iod/100 g. Rasio bahan terhadap volume pelarut terbaik untuk sampel B sebesar 1:8 dengan rendemen minyak tertinggi yaitu 75%. Minyak yang dihasilkan memiliki bilangan asam 2,77 mg KOH/g, densitas 0,87 g/cm3, viskositas 4,36 cSt, dan bilangan iod 40,61 g iod/100 g. Keywords: extraction, palm oil, recovery, reflux, spent bleaching earth
The Function and Context of Speaking in the Oral Tradition of the Kejhung Madura : A Sociopragmatic Study Sri Utami; Widayati, Wahyu; Victor Marolitua L Tobing
Open Access Indonesia Journal of Social Sciences Vol. 5 No. 1 (2022): Open Access Indonesia Journal of Social Sciences
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/oaijss.v5i1.104

Abstract

The purpose of this study is the function of speech acts in the Madurese kejhung oral tradition and the context behind the Madurese kejhung oral tradition, which includes situation, social, culture, and ideology. This study uses a sociopragmatic approach and descriptive method. Researchers as human instruments. This research involves hermeneutic and sociopragmatic analysis methods. Hermeneutics is used to reveal the conditions of the context. This study uses data in the form of kejhung text which contains the function of speech and the context of the Madurese kejhung oral tradition in the form of rhymes. The results of the study indicate that the speech function is dominated by the directive speech function, both the advisory directive function and the inviting or wishing directive function. This shows that the function of Madurese speech is to actualize the attitude of the Madurese. The context of the kejhung madura situation is the wedding ceremony, welcoming guests, cow race, sonok cow, and rokat tase'. The social context behind daily life such as harmony and cooperation, the cultural context is the customs and way of life, while the ideological context behind the Madura kejhung is strong Islamic teaching within the Madurese community.
Estetika Bahasa Indramayu pada Novel Aib dan Nasib Karya Minanto (Kajian Sosiolinguistik) Latifah, Suci Ayu; Sri Utami; Wahyu Widayati
Jurnal Ilmiah FONEMA : Jurnal Edukasi Bahasa dan Sastra Indonesia Vol 4 No 2 (2021)
Publisher : FKIP - Universitas Dr. Soetomo Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25139/fn.v4i2.4251

Abstract

Penelitian bertujuan untuk mendedah estetika bahasa Indramayu fokus pada variasi bahasa dari segi penutur dalam novel Aib dan Nasib karya Minanto. Hal ini dilakukan karena teks novel banyak menyisipkan warna bahasa dalam stilistika penceritaan. Aib dan Nasib menceritakan kehidupan masyarakat Indramayu dari keseluruhan aktivitasnya. Mulai dari interaksi sosial, sistem kekerabatan, hingga kultur masyarakat. Metode penelitian menggunakan deskriptif kualitatif, dengan objek penelitian Aib dan Nasib karya Minanto. Adapun teknik menggunakan studi pustaka dengan teknik deskriptif analitis sebagai teknik analisis data. Hasil temuan menunjukkan variasi bahasa seperti dialek, ideolek, kronolek, dan sosiolek. Pengemasan estetika bahasa tampak dari kreativitas pengarang melalui dialog, narasi, dan deskripsi. Estetika bahasa dalam novel menunjukkan adanya hubungan antara penggunaan bahasa dengan sekelompok masyarakat.
The Function and Context of Speaking in the Oral Tradition of the Kejhung Madura : A Sociopragmatic Study Sri Utami; Widayati, Wahyu; Victor Marolitua L Tobing
Open Access Indonesia Journal of Social Sciences Vol. 5 No. 1 (2022): Open Access Indonesia Journal of Social Sciences
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.449 KB) | DOI: 10.37275/oaijss.v5i1.104

Abstract

The purpose of this study is the function of speech acts in the Madurese kejhung oral tradition and the context behind the Madurese kejhung oral tradition, which includes situation, social, culture, and ideology. This study uses a sociopragmatic approach and descriptive method. Researchers as human instruments. This research involves hermeneutic and sociopragmatic analysis methods. Hermeneutics is used to reveal the conditions of the context. This study uses data in the form of kejhung text which contains the function of speech and the context of the Madurese kejhung oral tradition in the form of rhymes. The results of the study indicate that the speech function is dominated by the directive speech function, both the advisory directive function and the inviting or wishing directive function. This shows that the function of Madurese speech is to actualize the attitude of the Madurese. The context of the kejhung madura situation is the wedding ceremony, welcoming guests, cow race, sonok cow, and rokat tase'. The social context behind daily life such as harmony and cooperation, the cultural context is the customs and way of life, while the ideological context behind the Madura kejhung is strong Islamic teaching within the Madurese community.